Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes
Biomimetic functionalization to confer stealth and targeting properties to nanoparticles is a field of intense study. Extracellular vesicles (EV), sub-micron delivery vehicles for intercellular communication, have unique characteristics for drug delivery. We investigated the top-down functionalizati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/8/1797 |
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| author | Jan Van Deun Quentin Roux Sarah Deville Thibaut Van Acker Pekka Rappu Ilkka Miinalainen Jyrki Heino Frank Vanhaecke Bruno G. De Geest Olivier De Wever An Hendrix |
| author_facet | Jan Van Deun Quentin Roux Sarah Deville Thibaut Van Acker Pekka Rappu Ilkka Miinalainen Jyrki Heino Frank Vanhaecke Bruno G. De Geest Olivier De Wever An Hendrix |
| author_sort | Jan Van Deun |
| collection | DOAJ |
| description | Biomimetic functionalization to confer stealth and targeting properties to nanoparticles is a field of intense study. Extracellular vesicles (EV), sub-micron delivery vehicles for intercellular communication, have unique characteristics for drug delivery. We investigated the top-down functionalization of gold nanoparticles with extracellular vesicle membranes, including both lipids and associated membrane proteins, through mechanical extrusion. EV surface-exposed membrane proteins were confirmed to help avoid unwanted elimination by macrophages, while improving autologous uptake. EV membrane morphology, protein composition and orientation were found to be unaffected by mechanical extrusion. We implemented complementary EV characterization methods, including transmission- and immune-electron microscopy, and nanoparticle tracking analysis, to verify membrane coating, size and zeta potential of the EV membrane-cloaked nanoparticles. While successful EV membrane coating of the gold nanoparticles resulted in lower macrophage uptake, low yield was found to be a significant downside of the extrusion approach. Our data incentivize more research to leverage EV membrane biomimicking as a unique drug delivery approach in the near future. |
| first_indexed | 2024-03-10T18:08:11Z |
| format | Article |
| id | doaj.art-7954e922a9234bacbf83d6b8935b1207 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T18:08:11Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-7954e922a9234bacbf83d6b8935b12072023-11-20T08:18:25ZengMDPI AGCells2073-44092020-07-0198179710.3390/cells9081797Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle MembranesJan Van Deun0Quentin Roux1Sarah Deville2Thibaut Van Acker3Pekka Rappu4Ilkka Miinalainen5Jyrki Heino6Frank Vanhaecke7Bruno G. De Geest8Olivier De Wever9An Hendrix10Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, BelgiumLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, BelgiumLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, BelgiumDepartment of Analytical Chemistry, Ghent University, 9000 Ghent, BelgiumDepartment of Biochemistry, University of Turku, 20500 Turku, FinlandBiocenter Oulu, Department of Pathology, Oulu University Hospital, University of Oulu, 90220 Oulu, FinlandDepartment of Biochemistry, University of Turku, 20500 Turku, FinlandDepartment of Analytical Chemistry, Ghent University, 9000 Ghent, BelgiumDepartment of Pharmaceutics, Ghent University, 9000 Ghent, BelgiumLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, BelgiumLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, 9000 Ghent, BelgiumBiomimetic functionalization to confer stealth and targeting properties to nanoparticles is a field of intense study. Extracellular vesicles (EV), sub-micron delivery vehicles for intercellular communication, have unique characteristics for drug delivery. We investigated the top-down functionalization of gold nanoparticles with extracellular vesicle membranes, including both lipids and associated membrane proteins, through mechanical extrusion. EV surface-exposed membrane proteins were confirmed to help avoid unwanted elimination by macrophages, while improving autologous uptake. EV membrane morphology, protein composition and orientation were found to be unaffected by mechanical extrusion. We implemented complementary EV characterization methods, including transmission- and immune-electron microscopy, and nanoparticle tracking analysis, to verify membrane coating, size and zeta potential of the EV membrane-cloaked nanoparticles. While successful EV membrane coating of the gold nanoparticles resulted in lower macrophage uptake, low yield was found to be a significant downside of the extrusion approach. Our data incentivize more research to leverage EV membrane biomimicking as a unique drug delivery approach in the near future.https://www.mdpi.com/2073-4409/9/8/1797extracellular vesicleexosomemicrovesiclebiomimeticsgold nanoparticlefunctionalization |
| spellingShingle | Jan Van Deun Quentin Roux Sarah Deville Thibaut Van Acker Pekka Rappu Ilkka Miinalainen Jyrki Heino Frank Vanhaecke Bruno G. De Geest Olivier De Wever An Hendrix Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes Cells extracellular vesicle exosome microvesicle biomimetics gold nanoparticle functionalization |
| title | Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes |
| title_full | Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes |
| title_fullStr | Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes |
| title_full_unstemmed | Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes |
| title_short | Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes |
| title_sort | feasibility of mechanical extrusion to coat nanoparticles with extracellular vesicle membranes |
| topic | extracellular vesicle exosome microvesicle biomimetics gold nanoparticle functionalization |
| url | https://www.mdpi.com/2073-4409/9/8/1797 |
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