Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India
An alkaliphilic actinomycete, BCI-1, was isolated from soil samples collected from Saurashtra University campus, Gujarat. Isolated strain was identified as Streptomyces werraensis based on morphological, biochemical and phylogenetic analysis. Maximum antibiotic production was obtained in media conta...
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Elsevier
2014-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X1400006X |
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| author | Gaurav V. Sanghvi Dipak Ghevariya Subhash Gosai Riddhi Langa Niketa Dhaduk Prashant D. Kunjadia Devendra J. Vaishnav Gaurav S. Dave |
| author_facet | Gaurav V. Sanghvi Dipak Ghevariya Subhash Gosai Riddhi Langa Niketa Dhaduk Prashant D. Kunjadia Devendra J. Vaishnav Gaurav S. Dave |
| author_sort | Gaurav V. Sanghvi |
| collection | DOAJ |
| description | An alkaliphilic actinomycete, BCI-1, was isolated from soil samples collected from Saurashtra University campus, Gujarat. Isolated strain was identified as Streptomyces werraensis based on morphological, biochemical and phylogenetic analysis. Maximum antibiotic production was obtained in media containing sucrose 2%, Yeast extract 1.5%, and NaCl 2.5% at pH 9.0 for 7 days at 30 °C. Maximum inhibitory compound was produced at pH 9 and at 30 °C. FTIR revealed imine, amine, alkane (CC) of aromatic ring and p-di substituted benzene, whereas HPLC analysis of partially purified compound and library search confirmed 95% peaks matches with erythromycin. Chloroform extracted isolated compound showed MIC values 1 μg/ml against Bacillus subtilis, ≤0.5 μg/ml against Staphylococcus aureus, ≤0.5 μg/ml against Escherichia coli and 2.0 μg/ml against Serretia GSD2 sp., which is more effective in comparison to ehtylacetate and methanol extracted compounds. The study holds significance as only few alkaliphilic actinomycetes have been explored for their antimicrobial potential. |
| first_indexed | 2024-04-12T19:38:47Z |
| format | Article |
| id | doaj.art-7954f604baf944479a82f64b3ac8236b |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T19:38:47Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-7954f604baf944479a82f64b3ac8236b2022-12-22T03:19:09ZengElsevierBiotechnology Reports2215-017X2014-06-011C2710.1016/j.btre.2014.05.003Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, IndiaGaurav V. Sanghvi0Dipak Ghevariya1Subhash Gosai2Riddhi Langa3Niketa Dhaduk4Prashant D. Kunjadia5Devendra J. Vaishnav6Gaurav S. Dave7Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, IndiaDepartment of Biochemistry, Saurashtra University, Rajkot, IndiaDepartment of Biochemistry, Saurashtra University, Rajkot, IndiaDepartment of Biochemistry, Saurashtra University, Rajkot, IndiaB. N. Patel Institute of Paramedical Sciences, Bhalej Road, Anand, IndiaB. N. Patel Institute of Paramedical Sciences, Bhalej Road, Anand, IndiaDepartment of Pharmaceutical Sciences, Saurashtra University, Rajkot, IndiaDepartment of Biochemistry, Saurashtra University, Rajkot, IndiaAn alkaliphilic actinomycete, BCI-1, was isolated from soil samples collected from Saurashtra University campus, Gujarat. Isolated strain was identified as Streptomyces werraensis based on morphological, biochemical and phylogenetic analysis. Maximum antibiotic production was obtained in media containing sucrose 2%, Yeast extract 1.5%, and NaCl 2.5% at pH 9.0 for 7 days at 30 °C. Maximum inhibitory compound was produced at pH 9 and at 30 °C. FTIR revealed imine, amine, alkane (CC) of aromatic ring and p-di substituted benzene, whereas HPLC analysis of partially purified compound and library search confirmed 95% peaks matches with erythromycin. Chloroform extracted isolated compound showed MIC values 1 μg/ml against Bacillus subtilis, ≤0.5 μg/ml against Staphylococcus aureus, ≤0.5 μg/ml against Escherichia coli and 2.0 μg/ml against Serretia GSD2 sp., which is more effective in comparison to ehtylacetate and methanol extracted compounds. The study holds significance as only few alkaliphilic actinomycetes have been explored for their antimicrobial potential.http://www.sciencedirect.com/science/article/pii/S2215017X1400006XActinomycetesStreptomycesErythromycinFermentation |
| spellingShingle | Gaurav V. Sanghvi Dipak Ghevariya Subhash Gosai Riddhi Langa Niketa Dhaduk Prashant D. Kunjadia Devendra J. Vaishnav Gaurav S. Dave Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India Biotechnology Reports Actinomycetes Streptomyces Erythromycin Fermentation |
| title | Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India |
| title_full | Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India |
| title_fullStr | Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India |
| title_full_unstemmed | Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India |
| title_short | Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India |
| title_sort | isolation and partial purification of erythromycin from alkaliphilic streptomyces werraensis isolated from rajkot india |
| topic | Actinomycetes Streptomyces Erythromycin Fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X1400006X |
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