Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay
The Helicoverpa armigera(Noctuidae: Heliothinae) is a polyphagous and cosmopolitan pest distributed throughout South America. The objective of this research was to identify by PCR-RFLP molecular analysis, the presence of H. armigerain the Amambay region in Paraguay. Delta trapswith sexual pheromone...
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Asunción
2018-07-01
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| Series: | Investigación Agraria |
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| Online Access: | http://www.agr.una.py/revista/index.php/ria/article/view/512 |
| _version_ | 1818287353409568768 |
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| author | Marcos Arturo Ferreira Agüero Enson Gusman Sosa Osmar René Arias |
| author_facet | Marcos Arturo Ferreira Agüero Enson Gusman Sosa Osmar René Arias |
| author_sort | Marcos Arturo Ferreira Agüero |
| collection | DOAJ |
| description | The Helicoverpa armigera(Noctuidae: Heliothinae) is a polyphagous and cosmopolitan pest distributed throughout South America. The objective of this research was to identify by PCR-RFLP molecular analysis, the presence of H. armigerain the Amambay region in Paraguay. Delta trapswith sexual pheromone were deployed in the 2016/2017 season soybean crop distributed at the following locations: Pedro Juan Caballero, Zanja Pytã, Capitán Bado, Karapã’ĩ and Bella Vista Norte. Sampling cloth was used for collecting caterpillars. The collected specimens were sorted by external morphological characters, individualized in labeled jars containing pure alcohol. 24 samples were sent to the Arthropods Molecular Ecology Laboratory ESALQ/USP for identification by PCR-RFLP molecular analysis. Ten specimens of H. armigerabelonging to Fortuna and Zanja Pytã were identified. Ten other specimens were identified as H. zeabelonging to Cerro Cora'i, Captain Bado, Cerro Kuatiá and Karapã’ĩ. The remaining four samples did not amplify by PCR-RFLP. The analysis revealed the occurrence of H. armigerain soybean and maize crops in the northern region of Paraguay |
| first_indexed | 2024-12-13T01:39:08Z |
| format | Article |
| id | doaj.art-7955ac50c7154b6e8475aa4d9acbf337 |
| institution | Directory Open Access Journal |
| issn | 1684-9086 2305-0683 |
| language | English |
| last_indexed | 2024-12-13T01:39:08Z |
| publishDate | 2018-07-01 |
| publisher | Universidad Nacional de Asunción |
| record_format | Article |
| series | Investigación Agraria |
| spelling | doaj.art-7955ac50c7154b6e8475aa4d9acbf3372022-12-22T00:03:49ZengUniversidad Nacional de AsunciónInvestigación Agraria1684-90862305-06832018-07-01201849010.18004/investig.agrar.2018.junio.84-90Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, ParaguayMarcos Arturo Ferreira Agüero0Enson Gusman Sosa1Osmar René Arias2Universidad Nacional de Asunción, Facultad de Ciencias Agrarias, Área de Protección Vegetal, Filial Pedro Juan Caballero. Pedro Juan Caballero, ParaguayUniversidad Nacional de Asunción, Facultad de Ciencias Agrarias, Ingeniería Agronómica, Filial Pedro Juan Caballero. Pedro Juan Caballero, Paraguay.Universidad Nacional de Asunción, Facultad de Ciencias Agrarias. San Lorenzo, Paraguay.The Helicoverpa armigera(Noctuidae: Heliothinae) is a polyphagous and cosmopolitan pest distributed throughout South America. The objective of this research was to identify by PCR-RFLP molecular analysis, the presence of H. armigerain the Amambay region in Paraguay. Delta trapswith sexual pheromone were deployed in the 2016/2017 season soybean crop distributed at the following locations: Pedro Juan Caballero, Zanja Pytã, Capitán Bado, Karapã’ĩ and Bella Vista Norte. Sampling cloth was used for collecting caterpillars. The collected specimens were sorted by external morphological characters, individualized in labeled jars containing pure alcohol. 24 samples were sent to the Arthropods Molecular Ecology Laboratory ESALQ/USP for identification by PCR-RFLP molecular analysis. Ten specimens of H. armigerabelonging to Fortuna and Zanja Pytã were identified. Ten other specimens were identified as H. zeabelonging to Cerro Cora'i, Captain Bado, Cerro Kuatiá and Karapã’ĩ. The remaining four samples did not amplify by PCR-RFLP. The analysis revealed the occurrence of H. armigerain soybean and maize crops in the northern region of Paraguayhttp://www.agr.una.py/revista/index.php/ria/article/view/512h. armigerapheromonedelta trapmolecular identification |
| spellingShingle | Marcos Arturo Ferreira Agüero Enson Gusman Sosa Osmar René Arias Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay Investigación Agraria h. armigera pheromone delta trap molecular identification |
| title | Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay |
| title_full | Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay |
| title_fullStr | Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay |
| title_full_unstemmed | Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay |
| title_short | Identificación molecular de Helicoverpa armígera (Noctuidae: Heliothinae) en el departamento de Amambay, Paraguay |
| title_sort | identificacion molecular de helicoverpa armigera noctuidae heliothinae en el departamento de amambay paraguay |
| topic | h. armigera pheromone delta trap molecular identification |
| url | http://www.agr.una.py/revista/index.php/ria/article/view/512 |
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