Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency
Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in par...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01045/full |
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| author | Han Yu Jian Yang Yafei Shi Jimmonique Donelson Sean M. Thompson Stuart Sprague Tony Roshan Da-Li Wang Jianzhong Liu Sunghun Park Paul A. Nakata Erin L. Connolly Kendal D. Hirschi Kendal D. Hirschi Michael A. Grusak Michael A. Grusak Ninghui Cheng |
| author_facet | Han Yu Jian Yang Yafei Shi Jimmonique Donelson Sean M. Thompson Stuart Sprague Tony Roshan Da-Li Wang Jianzhong Liu Sunghun Park Paul A. Nakata Erin L. Connolly Kendal D. Hirschi Kendal D. Hirschi Michael A. Grusak Michael A. Grusak Ninghui Cheng |
| author_sort | Han Yu |
| collection | DOAJ |
| description | Iron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex regulatory network to respond to conditions of iron limitations. However, the mechanisms that couple iron deficiency and oxidative stress responses are not fully understood. Here, we report the discovery that an Arabidopsis thaliana monothiol glutaredoxin S17 (AtGRXS17) plays a critical role in the plants ability to respond to iron deficiency stress and maintain redox homeostasis. In a yeast expression assay, AtGRXS17 was able to suppress the iron accumulation in yeast ScGrx3/ScGrx4 mutant cells. Genetic analysis indicated that plants with reduced AtGRXS17 expression were hypersensitive to iron deficiency and showed increased iron concentrations in mature seeds. Disruption of AtGRXS17 caused plant sensitivity to exogenous oxidants and increased ROS production under iron deficiency. Addition of reduced glutathione rescued the growth and alleviates the sensitivity of atgrxs17 mutants to iron deficiency. These findings suggest AtGRXS17 helps integrate redox homeostasis and iron deficiency responses. |
| first_indexed | 2024-12-13T09:48:34Z |
| format | Article |
| id | doaj.art-795664211d074398bdd22ccd84d28446 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T09:48:34Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-795664211d074398bdd22ccd84d284462022-12-21T23:51:59ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.01045274897Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron DeficiencyHan Yu0Jian Yang1Yafei Shi2Jimmonique Donelson3Sean M. Thompson4Stuart Sprague5Tony Roshan6Da-Li Wang7Jianzhong Liu8Sunghun Park9Paul A. Nakata10Erin L. Connolly11Kendal D. Hirschi12Kendal D. Hirschi13Michael A. Grusak14Michael A. Grusak15Ninghui Cheng16USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesCollege of Chemistry and Life Science, Zhejiang Normal UniversityJinhua, ChinaUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesDepartment of Horticultural Sciences, Texas A&M University, College StationTX, United StatesDepartment of Horticulture, Forestry and Recreation Resources, Kansas State University, ManhattanKS, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesCollege of Chemistry and Life Science, Zhejiang Normal UniversityJinhua, ChinaCollege of Chemistry and Life Science, Zhejiang Normal UniversityJinhua, ChinaDepartment of Horticulture, Forestry and Recreation Resources, Kansas State University, ManhattanKS, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesDepartment of Plant Science, Penn State University, University ParkPA, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesVegetable and Fruit Improvement Center, Texas A&M University, College StationTX, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesUSDA/ARS Red River Valley Agricultural Research Center, FargoND, United StatesUSDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, HoustonTX, United StatesIron (Fe) is an essential mineral nutrient and a metal cofactor required for many proteins and enzymes involved in the processes of DNA synthesis, respiration, and photosynthesis. Iron limitation can have detrimental effects on plant growth and development. Such effects are mediated, at least in part, through the generation of reactive oxygen species (ROS). Thus, plants have evolved a complex regulatory network to respond to conditions of iron limitations. However, the mechanisms that couple iron deficiency and oxidative stress responses are not fully understood. Here, we report the discovery that an Arabidopsis thaliana monothiol glutaredoxin S17 (AtGRXS17) plays a critical role in the plants ability to respond to iron deficiency stress and maintain redox homeostasis. In a yeast expression assay, AtGRXS17 was able to suppress the iron accumulation in yeast ScGrx3/ScGrx4 mutant cells. Genetic analysis indicated that plants with reduced AtGRXS17 expression were hypersensitive to iron deficiency and showed increased iron concentrations in mature seeds. Disruption of AtGRXS17 caused plant sensitivity to exogenous oxidants and increased ROS production under iron deficiency. Addition of reduced glutathione rescued the growth and alleviates the sensitivity of atgrxs17 mutants to iron deficiency. These findings suggest AtGRXS17 helps integrate redox homeostasis and iron deficiency responses.http://journal.frontiersin.org/article/10.3389/fpls.2017.01045/fulliron deficiencyoxidative stressredox homeostasisglutaredoxinArabidopsis |
| spellingShingle | Han Yu Jian Yang Yafei Shi Jimmonique Donelson Sean M. Thompson Stuart Sprague Tony Roshan Da-Li Wang Jianzhong Liu Sunghun Park Paul A. Nakata Erin L. Connolly Kendal D. Hirschi Kendal D. Hirschi Michael A. Grusak Michael A. Grusak Ninghui Cheng Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency Frontiers in Plant Science iron deficiency oxidative stress redox homeostasis glutaredoxin Arabidopsis |
| title | Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency |
| title_full | Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency |
| title_fullStr | Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency |
| title_full_unstemmed | Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency |
| title_short | Arabidopsis Glutaredoxin S17 Contributes to Vegetative Growth, Mineral Accumulation, and Redox Balance during Iron Deficiency |
| title_sort | arabidopsis glutaredoxin s17 contributes to vegetative growth mineral accumulation and redox balance during iron deficiency |
| topic | iron deficiency oxidative stress redox homeostasis glutaredoxin Arabidopsis |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01045/full |
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