Lipidomics Reveals a Tissue-Specific Fingerprint
In biological systems lipids generate membranes and have a key role in cell signaling and energy storage. Therefore, there is a wide diversity of molecular lipid expressed at the compositional level in cell membranes and organelles, as well as in tissues, whose lipid distribution remains unclear. He...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.01165/full |
| _version_ | 1818499660120064000 |
|---|---|
| author | Irene Pradas Kevin Huynh Rosanna Cabré Victòria Ayala Peter J. Meikle Mariona Jové Reinald Pamplona |
| author_facet | Irene Pradas Kevin Huynh Rosanna Cabré Victòria Ayala Peter J. Meikle Mariona Jové Reinald Pamplona |
| author_sort | Irene Pradas |
| collection | DOAJ |
| description | In biological systems lipids generate membranes and have a key role in cell signaling and energy storage. Therefore, there is a wide diversity of molecular lipid expressed at the compositional level in cell membranes and organelles, as well as in tissues, whose lipid distribution remains unclear. Here, we report a mass spectrometry study of lipid abundance across 7 rat tissues, detecting and quantifying 652 lipid molecular species from the glycerolipid, glycerophospholipid, fatty acyl, sphingolipid, sterol lipid and prenol lipid categories. Our results demonstrate that every tissue analyzed presents a specific lipid distribution and concentration. Thus, glycerophospholipids are the most abundant tissue lipid, they share a similar tissue distribution but differ in particular lipid species between tissues. Sphingolipids are more concentrated in the renal cortex and sterol lipids can be found mainly in both liver and kidney. Both types of white adipose tissue, visceral and subcutaneous, are rich in glycerolipids but differing the amount. Acylcarnitines are mainly in the skeletal muscle, gluteus and soleus, while heart presents higher levels of ubiquinone than other tissues. The present study demonstrates the existence of a rat tissue-specific fingerprint. |
| first_indexed | 2024-12-10T20:32:28Z |
| format | Article |
| id | doaj.art-795716c77a874554bf6bf18a58a61450 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T20:32:28Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-795716c77a874554bf6bf18a58a614502022-12-22T01:34:37ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-08-01910.3389/fphys.2018.01165387781Lipidomics Reveals a Tissue-Specific FingerprintIrene Pradas0Kevin Huynh1Rosanna Cabré2Victòria Ayala3Peter J. Meikle4Mariona Jové5Reinald Pamplona6Department of Experimental Medicine, Institute for Research in Biomedicine of Lleida, University of Lleida, Lleida, SpainBaker Heart and Diabetes Institute, Melbourne, VIC, AustraliaDepartment of Experimental Medicine, Institute for Research in Biomedicine of Lleida, University of Lleida, Lleida, SpainDepartment of Experimental Medicine, Institute for Research in Biomedicine of Lleida, University of Lleida, Lleida, SpainBaker Heart and Diabetes Institute, Melbourne, VIC, AustraliaDepartment of Experimental Medicine, Institute for Research in Biomedicine of Lleida, University of Lleida, Lleida, SpainDepartment of Experimental Medicine, Institute for Research in Biomedicine of Lleida, University of Lleida, Lleida, SpainIn biological systems lipids generate membranes and have a key role in cell signaling and energy storage. Therefore, there is a wide diversity of molecular lipid expressed at the compositional level in cell membranes and organelles, as well as in tissues, whose lipid distribution remains unclear. Here, we report a mass spectrometry study of lipid abundance across 7 rat tissues, detecting and quantifying 652 lipid molecular species from the glycerolipid, glycerophospholipid, fatty acyl, sphingolipid, sterol lipid and prenol lipid categories. Our results demonstrate that every tissue analyzed presents a specific lipid distribution and concentration. Thus, glycerophospholipids are the most abundant tissue lipid, they share a similar tissue distribution but differ in particular lipid species between tissues. Sphingolipids are more concentrated in the renal cortex and sterol lipids can be found mainly in both liver and kidney. Both types of white adipose tissue, visceral and subcutaneous, are rich in glycerolipids but differing the amount. Acylcarnitines are mainly in the skeletal muscle, gluteus and soleus, while heart presents higher levels of ubiquinone than other tissues. The present study demonstrates the existence of a rat tissue-specific fingerprint.https://www.frontiersin.org/article/10.3389/fphys.2018.01165/fulladult rat tissueslipidomicsglycerophospholipidsplasmalogensglycerolipidssphingolipids |
| spellingShingle | Irene Pradas Kevin Huynh Rosanna Cabré Victòria Ayala Peter J. Meikle Mariona Jové Reinald Pamplona Lipidomics Reveals a Tissue-Specific Fingerprint Frontiers in Physiology adult rat tissues lipidomics glycerophospholipids plasmalogens glycerolipids sphingolipids |
| title | Lipidomics Reveals a Tissue-Specific Fingerprint |
| title_full | Lipidomics Reveals a Tissue-Specific Fingerprint |
| title_fullStr | Lipidomics Reveals a Tissue-Specific Fingerprint |
| title_full_unstemmed | Lipidomics Reveals a Tissue-Specific Fingerprint |
| title_short | Lipidomics Reveals a Tissue-Specific Fingerprint |
| title_sort | lipidomics reveals a tissue specific fingerprint |
| topic | adult rat tissues lipidomics glycerophospholipids plasmalogens glycerolipids sphingolipids |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.01165/full |
| work_keys_str_mv | AT irenepradas lipidomicsrevealsatissuespecificfingerprint AT kevinhuynh lipidomicsrevealsatissuespecificfingerprint AT rosannacabre lipidomicsrevealsatissuespecificfingerprint AT victoriaayala lipidomicsrevealsatissuespecificfingerprint AT peterjmeikle lipidomicsrevealsatissuespecificfingerprint AT marionajove lipidomicsrevealsatissuespecificfingerprint AT reinaldpamplona lipidomicsrevealsatissuespecificfingerprint |