Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening
Flavivirus infections, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), present significant global public health challenges. For successful vaccine design, the assessment of neutralizing antibody activity requires reliable and robust methodologies for determin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/1/8 |
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| author | Kazumi Haga Zhenying (Nancy) Chen Misao Himeno Ryuichi Majima Meng Ling Moi |
| author_facet | Kazumi Haga Zhenying (Nancy) Chen Misao Himeno Ryuichi Majima Meng Ling Moi |
| author_sort | Kazumi Haga |
| collection | DOAJ |
| description | Flavivirus infections, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), present significant global public health challenges. For successful vaccine design, the assessment of neutralizing antibody activity requires reliable and robust methodologies for determining antibody titers. Although the plaque reduction neutralization test (PRNT) is commonly acknowledged as the gold standard, it has limitations in terms of time and cost, and its usage may be limited in resource-limited settings. To address these challenges, we introduced the micro-neutralization test (MNT) as a simplified alternative to the PRNT. The MNT employs a 96-well plate format, conducts microscale neutralization assays, and assesses cell viability by dissolving cells to create a uniform color solution, which is measured with a spectrometer. In this study, we evaluated the utility of the MNT by contrasting the end-point titers of the MNT and PRNT using 4 monoclonal antibodies, 15 non-human primate serum samples, and 2 therapeutic drug candidates across flaviviruses. The results demonstrated a strong correlation between the MNT and PRNT titers, affirming the robustness and reproducibility of the MNT for evaluating control measures against flaviviruses. This research contributes valuable insights toward the development of a cost-effective antibody titer testing approach that is particularly suitable for resource-limited settings. |
| first_indexed | 2024-03-08T10:38:27Z |
| format | Article |
| id | doaj.art-7957ee83c0d146189af4b6ccc17d3169 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-08T10:38:27Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7957ee83c0d146189af4b6ccc17d31692024-01-26T18:02:46ZengMDPI AGPathogens2076-08172023-12-01131810.3390/pathogens13010008Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug ScreeningKazumi Haga0Zhenying (Nancy) Chen1Misao Himeno2Ryuichi Majima3Meng Ling Moi4Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, JapanDepartment of Biology, Emory College of Art and Science, Emory University, Atlanta, GA 30322, USADepartment of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, JapanDepartment of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, JapanDepartment of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, JapanFlavivirus infections, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), present significant global public health challenges. For successful vaccine design, the assessment of neutralizing antibody activity requires reliable and robust methodologies for determining antibody titers. Although the plaque reduction neutralization test (PRNT) is commonly acknowledged as the gold standard, it has limitations in terms of time and cost, and its usage may be limited in resource-limited settings. To address these challenges, we introduced the micro-neutralization test (MNT) as a simplified alternative to the PRNT. The MNT employs a 96-well plate format, conducts microscale neutralization assays, and assesses cell viability by dissolving cells to create a uniform color solution, which is measured with a spectrometer. In this study, we evaluated the utility of the MNT by contrasting the end-point titers of the MNT and PRNT using 4 monoclonal antibodies, 15 non-human primate serum samples, and 2 therapeutic drug candidates across flaviviruses. The results demonstrated a strong correlation between the MNT and PRNT titers, affirming the robustness and reproducibility of the MNT for evaluating control measures against flaviviruses. This research contributes valuable insights toward the development of a cost-effective antibody titer testing approach that is particularly suitable for resource-limited settings.https://www.mdpi.com/2076-0817/13/1/8flavivirusmicroneutralization testneutralizing antibodiesdengueJapanese encephalitis virusZika |
| spellingShingle | Kazumi Haga Zhenying (Nancy) Chen Misao Himeno Ryuichi Majima Meng Ling Moi Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening Pathogens flavivirus microneutralization test neutralizing antibodies dengue Japanese encephalitis virus Zika |
| title | Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening |
| title_full | Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening |
| title_fullStr | Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening |
| title_full_unstemmed | Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening |
| title_short | Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening |
| title_sort | utility of an in vitro micro neutralizing test in comparison to a plaque reduction neutralization test for dengue virus japanese encephalitis virus and zika virus serology and drug screening |
| topic | flavivirus microneutralization test neutralizing antibodies dengue Japanese encephalitis virus Zika |
| url | https://www.mdpi.com/2076-0817/13/1/8 |
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