Adipose transcriptome in the scalp of androgenetic alopecia
Previous studies have shown how adipocytes can modulate the activity of hair follicle stem cells. However, the role of adipocytes in the pathogenesis of androgenetic alopecia (AGA) remains unknown. We aimed to determine signaling pathways related to the adipose tissue changes in the human scalp with...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2023.1195656/full |
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| author | Criselda Jean G. Cruz Criselda Jean G. Cruz Yi-Kai Hong Yi-Kai Hong Wilson Jr. F. Aala Ren-Yeu Tsai Pei-Lun Chung Yau-Sheng Tsai Chao-Kai Hsu Chao-Kai Hsu Chao-Chun Yang Chao-Chun Yang |
| author_facet | Criselda Jean G. Cruz Criselda Jean G. Cruz Yi-Kai Hong Yi-Kai Hong Wilson Jr. F. Aala Ren-Yeu Tsai Pei-Lun Chung Yau-Sheng Tsai Chao-Kai Hsu Chao-Kai Hsu Chao-Chun Yang Chao-Chun Yang |
| author_sort | Criselda Jean G. Cruz |
| collection | DOAJ |
| description | Previous studies have shown how adipocytes can modulate the activity of hair follicle stem cells. However, the role of adipocytes in the pathogenesis of androgenetic alopecia (AGA) remains unknown. We aimed to determine signaling pathways related to the adipose tissue changes in the human scalp with AGA through RNA-seq analysis. RNA was isolated from the adipose tissues derived from the bald (frontal) and normal (occipital) scalps of male patients with AGA (n = 4). The pooled RNA extracts from these samples were subjected to RNA sequencing, followed by differential gene expression and pathway analysis. Our gene expression analysis identified 1,060 differentially expressed genes, including 522 upregulated and 538 downregulated genes in the bald AGA scalp. Biological pathways pertaining to either adipose tissue metabolism or the hair cycle were generated in our pathway analysis. Downregulation of the peroxisome proliferator-activated receptor (PPAR) signaling pathway was noted to be significant in the bald scalp. Expression of adipogenic markers (e.g., PPARG, FABP4, PLN1, and ADIPOQ) was also decreased in the bald site. These findings imply that adipogenesis becomes downregulated in AGA, specifically within the bald scalp adipose. Our results lead to the hypothesis that PPARγ-mediated adipogenesis in the scalp adipose, via crosstalk with signaling pathways involved in hair cycling, might play a role in AGA. |
| first_indexed | 2024-03-12T01:59:21Z |
| format | Article |
| id | doaj.art-795dfe56438c41dfb8939b79366163b8 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-12T01:59:21Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-795dfe56438c41dfb8939b79366163b82023-09-07T20:57:25ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-09-011010.3389/fmed.2023.11956561195656Adipose transcriptome in the scalp of androgenetic alopeciaCriselda Jean G. Cruz0Criselda Jean G. Cruz1Yi-Kai Hong2Yi-Kai Hong3Wilson Jr. F. Aala4Ren-Yeu Tsai5Pei-Lun Chung6Yau-Sheng Tsai7Chao-Kai Hsu8Chao-Kai Hsu9Chao-Chun Yang10Chao-Chun Yang11Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanInternational Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TaiwanDepartment of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanInternational Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TaiwanInstitute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Dermatology and Skin Laser Center, Taipei Municipal Wan-Fang Hospital, Taipei Medical University, Taipei, TaiwanDepartment of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanInstitute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanInternational Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TaiwanDepartment of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanInternational Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TaiwanPrevious studies have shown how adipocytes can modulate the activity of hair follicle stem cells. However, the role of adipocytes in the pathogenesis of androgenetic alopecia (AGA) remains unknown. We aimed to determine signaling pathways related to the adipose tissue changes in the human scalp with AGA through RNA-seq analysis. RNA was isolated from the adipose tissues derived from the bald (frontal) and normal (occipital) scalps of male patients with AGA (n = 4). The pooled RNA extracts from these samples were subjected to RNA sequencing, followed by differential gene expression and pathway analysis. Our gene expression analysis identified 1,060 differentially expressed genes, including 522 upregulated and 538 downregulated genes in the bald AGA scalp. Biological pathways pertaining to either adipose tissue metabolism or the hair cycle were generated in our pathway analysis. Downregulation of the peroxisome proliferator-activated receptor (PPAR) signaling pathway was noted to be significant in the bald scalp. Expression of adipogenic markers (e.g., PPARG, FABP4, PLN1, and ADIPOQ) was also decreased in the bald site. These findings imply that adipogenesis becomes downregulated in AGA, specifically within the bald scalp adipose. Our results lead to the hypothesis that PPARγ-mediated adipogenesis in the scalp adipose, via crosstalk with signaling pathways involved in hair cycling, might play a role in AGA.https://www.frontiersin.org/articles/10.3389/fmed.2023.1195656/fullandrogenetic alopeciahair follicleadipogenesisPPAR signaling pathwaytranscriptome |
| spellingShingle | Criselda Jean G. Cruz Criselda Jean G. Cruz Yi-Kai Hong Yi-Kai Hong Wilson Jr. F. Aala Ren-Yeu Tsai Pei-Lun Chung Yau-Sheng Tsai Chao-Kai Hsu Chao-Kai Hsu Chao-Chun Yang Chao-Chun Yang Adipose transcriptome in the scalp of androgenetic alopecia Frontiers in Medicine androgenetic alopecia hair follicle adipogenesis PPAR signaling pathway transcriptome |
| title | Adipose transcriptome in the scalp of androgenetic alopecia |
| title_full | Adipose transcriptome in the scalp of androgenetic alopecia |
| title_fullStr | Adipose transcriptome in the scalp of androgenetic alopecia |
| title_full_unstemmed | Adipose transcriptome in the scalp of androgenetic alopecia |
| title_short | Adipose transcriptome in the scalp of androgenetic alopecia |
| title_sort | adipose transcriptome in the scalp of androgenetic alopecia |
| topic | androgenetic alopecia hair follicle adipogenesis PPAR signaling pathway transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2023.1195656/full |
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