Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes
The short-tailed phenotype has long been considered one of the best traits for population genetic improvement in sheep breeding. In short-tailed sheep, not only is tail fat eliminated but also the pubic area is exposed due to the lack of a tail covering, giving them an advantage in reproduction. Rec...
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MDPI AG
2022-03-01
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| author | Guang Yang Caiyun Wang Hong Su Daqing Wang Aolie Dou Lu Chen Teng Ma Moning Liu Jie Su Xiaojing Xu Yanyan Yang Tingyi He Xihe Li Yongli Song Guifang Cao |
| author_facet | Guang Yang Caiyun Wang Hong Su Daqing Wang Aolie Dou Lu Chen Teng Ma Moning Liu Jie Su Xiaojing Xu Yanyan Yang Tingyi He Xihe Li Yongli Song Guifang Cao |
| author_sort | Guang Yang |
| collection | DOAJ |
| description | The short-tailed phenotype has long been considered one of the best traits for population genetic improvement in sheep breeding. In short-tailed sheep, not only is tail fat eliminated but also the pubic area is exposed due to the lack of a tail covering, giving them an advantage in reproduction. Recent studies have shown that two linked mutations in sheep <i>TBXT</i> at nucleotides 333 and 334 are associated with the short-tailed phenotype. In the population of short-tailed sheep, several heterozygous mutants of this gene are found. In our research, we used high-resolution melting (HRM) to identify homozygous and heterozygous genotypes in a flock of short-tailed sheep and compared the results with those of Sanger sequencing, which were identical. This demonstrates that our established HRM method, a rapid and inexpensive genotyping method, can be used to identify homozygous and heterozygous individuals in short-tailed sheep flocks. |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:12:40Z |
| publishDate | 2022-03-01 |
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| spelling | doaj.art-7962904a6eba4b398042af5fe5f18b292023-11-24T00:10:10ZengMDPI AGAnimals2076-26152022-03-0112679210.3390/ani12060792Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> HeterozygotesGuang Yang0Caiyun Wang1Hong Su2Daqing Wang3Aolie Dou4Lu Chen5Teng Ma6Moning Liu7Jie Su8Xiaojing Xu9Yanyan Yang10Tingyi He11Xihe Li12Yongli Song13Guifang Cao14School of Life Sciences, Inner Mongolia University, Hohhot 010021, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaInstitute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, ChinaInstitute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, ChinaSchool of Life Sciences, Inner Mongolia University, Hohhot 010021, ChinaSchool of Life Sciences, Inner Mongolia University, Hohhot 010021, ChinaSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, ChinaThe short-tailed phenotype has long been considered one of the best traits for population genetic improvement in sheep breeding. In short-tailed sheep, not only is tail fat eliminated but also the pubic area is exposed due to the lack of a tail covering, giving them an advantage in reproduction. Recent studies have shown that two linked mutations in sheep <i>TBXT</i> at nucleotides 333 and 334 are associated with the short-tailed phenotype. In the population of short-tailed sheep, several heterozygous mutants of this gene are found. In our research, we used high-resolution melting (HRM) to identify homozygous and heterozygous genotypes in a flock of short-tailed sheep and compared the results with those of Sanger sequencing, which were identical. This demonstrates that our established HRM method, a rapid and inexpensive genotyping method, can be used to identify homozygous and heterozygous individuals in short-tailed sheep flocks.https://www.mdpi.com/2076-2615/12/6/792short-tailed traitsheep<i>TBXT</i>HRM |
| spellingShingle | Guang Yang Caiyun Wang Hong Su Daqing Wang Aolie Dou Lu Chen Teng Ma Moning Liu Jie Su Xiaojing Xu Yanyan Yang Tingyi He Xihe Li Yongli Song Guifang Cao Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes Animals short-tailed trait sheep <i>TBXT</i> HRM |
| title | Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes |
| title_full | Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes |
| title_fullStr | Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes |
| title_full_unstemmed | Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes |
| title_short | Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep <i>TBXT</i> Heterozygotes |
| title_sort | development and application of a high resolution melting analysis with unlabeled probes for the screening of short tailed sheep i tbxt i heterozygotes |
| topic | short-tailed trait sheep <i>TBXT</i> HRM |
| url | https://www.mdpi.com/2076-2615/12/6/792 |
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