Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice
Background: Leishmania major is the etiologic agent of zoonotic cutaneous leishmaniasis in Iran, and glucantime injection is currently used for its treatment. This study aimed to evaluate the anti-leishmanial effect of topical Plantago psyllium and white vinegar in L. major infected BALB/c mice....
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2022-11-01
|
| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/1091 |
| _version_ | 1811179852897714176 |
|---|---|
| author | Abdolali Moshfe Keianoush Karami Maryam Bahmani Mohsen Naghmachi Shahrbanoo Askarian Abbas Rezaei Roohallah Zare Ali Jamshidi |
| author_facet | Abdolali Moshfe Keianoush Karami Maryam Bahmani Mohsen Naghmachi Shahrbanoo Askarian Abbas Rezaei Roohallah Zare Ali Jamshidi |
| author_sort | Abdolali Moshfe |
| collection | DOAJ |
| description |
Background: Leishmania major is the etiologic agent of zoonotic cutaneous leishmaniasis in Iran, and glucantime injection is currently used for its treatment. This study aimed to evaluate the anti-leishmanial effect of topical Plantago psyllium and white vinegar in L. major infected BALB/c mice.
Methods: Thirty infected mice were divided into five groups as follows: Group 1: treated with the combination of ovata powder and white vinegar, Group 2: treated with glucantime, Group 3: treated with white vinegar, Group 4: treated with the combination of ovata powder and water, and Group 5: without any treatment. All the groups were treated for 18 days. Lesion size was measured, and final smears were prepared for microscopic examination.
Results: The findings indicated that the difference in the mean areas of the ulcers in all the groups before and after treatment was not significant, except for the second (glucantime) and third (vinegar) groups. Also, the results showed that in the first, second, third, and fourth group, 6 (60%), 4 (80%), 3 (60%), and 2 (40%) mice were healed, respectively. However, ulcers remained in all the five mice of the control group.
Conclusion: The combination of ovata powder and white vinegar has been traditionally used to treat leishmanial lesions in Iran. It seems the most anti-leishmanial effect is related to vinegar and supported by Plantago. The route of treatment with this combination is very simple and painless in comparison with injection. Thus, further studies on this issue could help to design more effective and easy-to-use drugs.
|
| first_indexed | 2024-04-11T06:41:32Z |
| format | Article |
| id | doaj.art-79660ad0611247eb857bf137f8f6005d |
| institution | Directory Open Access Journal |
| issn | 2322-1984 2322-2271 |
| language | English |
| last_indexed | 2024-04-11T06:41:32Z |
| publishDate | 2022-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-79660ad0611247eb857bf137f8f6005d2022-12-22T04:39:31ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases2322-19842322-22712022-11-0116110.18502/jad.v16i1.11191Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c MiceAbdolali Moshfe0Keianoush Karami1Maryam Bahmani2Mohsen Naghmachi3Shahrbanoo Askarian4Abbas Rezaei5Roohallah Zare6Ali Jamshidi7Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran, Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranBehbahan Faculty of Medical Sciences, Behbahan, Iran Background: Leishmania major is the etiologic agent of zoonotic cutaneous leishmaniasis in Iran, and glucantime injection is currently used for its treatment. This study aimed to evaluate the anti-leishmanial effect of topical Plantago psyllium and white vinegar in L. major infected BALB/c mice. Methods: Thirty infected mice were divided into five groups as follows: Group 1: treated with the combination of ovata powder and white vinegar, Group 2: treated with glucantime, Group 3: treated with white vinegar, Group 4: treated with the combination of ovata powder and water, and Group 5: without any treatment. All the groups were treated for 18 days. Lesion size was measured, and final smears were prepared for microscopic examination. Results: The findings indicated that the difference in the mean areas of the ulcers in all the groups before and after treatment was not significant, except for the second (glucantime) and third (vinegar) groups. Also, the results showed that in the first, second, third, and fourth group, 6 (60%), 4 (80%), 3 (60%), and 2 (40%) mice were healed, respectively. However, ulcers remained in all the five mice of the control group. Conclusion: The combination of ovata powder and white vinegar has been traditionally used to treat leishmanial lesions in Iran. It seems the most anti-leishmanial effect is related to vinegar and supported by Plantago. The route of treatment with this combination is very simple and painless in comparison with injection. Thus, further studies on this issue could help to design more effective and easy-to-use drugs. https://jad.tums.ac.ir/index.php/jad/article/view/1091Leishmaniasis; Plantago psyllium; Ulcer; Mice |
| spellingShingle | Abdolali Moshfe Keianoush Karami Maryam Bahmani Mohsen Naghmachi Shahrbanoo Askarian Abbas Rezaei Roohallah Zare Ali Jamshidi Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice Journal of Arthropod-Borne Diseases Leishmaniasis; Plantago psyllium; Ulcer; Mice |
| title | Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice |
| title_full | Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice |
| title_fullStr | Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice |
| title_full_unstemmed | Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice |
| title_short | Anti Leishmanial Effect of Plantago psyllium (Ovate) and White Vinegar on Leishmania major Lesion in BALB/c Mice |
| title_sort | anti leishmanial effect of plantago psyllium ovate and white vinegar on leishmania major lesion in balb c mice |
| topic | Leishmaniasis; Plantago psyllium; Ulcer; Mice |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/1091 |
| work_keys_str_mv | AT abdolalimoshfe antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT keianoushkarami antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT maryambahmani antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT mohsennaghmachi antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT shahrbanooaskarian antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT abbasrezaei antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT roohallahzare antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice AT alijamshidi antileishmanialeffectofplantagopsylliumovateandwhitevinegaronleishmaniamajorlesioninbalbcmice |