Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro
Cerebral edema is a major, life threatening complication of ischemic brain damage. Previous studies using brain slices have revealed that cellular swelling and a concomitant increase in tissue transparency starts within minutes of the onset of metabolic insult in association with collective anoxic s...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-12-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2018.00502/full |
| _version_ | 1819178190362378240 |
|---|---|
| author | Elvira Juzekaeva Azat Gainutdinov Marat Mukhtarov Roustem Khazipov Roustem Khazipov |
| author_facet | Elvira Juzekaeva Azat Gainutdinov Marat Mukhtarov Roustem Khazipov Roustem Khazipov |
| author_sort | Elvira Juzekaeva |
| collection | DOAJ |
| description | Cerebral edema is a major, life threatening complication of ischemic brain damage. Previous studies using brain slices have revealed that cellular swelling and a concomitant increase in tissue transparency starts within minutes of the onset of metabolic insult in association with collective anoxic spreading depolarization (aSD). However, the dynamics of tissue swelling in brain slices under ischemia-like conditions remain elusive. Here, we explored the dynamics of brain tissue swelling induced by oxygen-glucose deprivation (OGD) in submerged rat barrel cortex slices. Video monitoring of the vertical and horizontal position of fluorescent dye-filled neurons and contrast slice surface imaging revealed elevation of the slice surface and a horizontal displacement of the cortical tissue during OGD. The OGD-induced tissue movement was also associated with an expansion of the slice borders. Tissue swelling started several minutes after aSD and continued during reperfusion with normal solution. Thirty minutes after aSD, slice borders had expanded by ~130 μm and the slice surface had moved up to attain a height of ~70 μm above control levels, which corresponded to a volume increase of ~30%. Hyperosmotic sucrose solution partially reduced the OGD-induced slice swelling. Thus, OGD-induced cortical slice tissue swelling in brain slices in vitro recapitulates many features of ischemic cerebral edema in vivo, its onset is tightly linked to aSD and it develops at a relatively slow pace after aSD. We propose that this model of cerebral edema in vitro could be useful for the exploration of the pathophysiological mechanisms underlying ischemic cerebral edema and in the search for an efficient treatment to this devastating condition. |
| first_indexed | 2024-12-22T21:38:37Z |
| format | Article |
| id | doaj.art-796723144a4c4633b2f0c6de628f0ca5 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-22T21:38:37Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-796723144a4c4633b2f0c6de628f0ca52022-12-21T18:11:41ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022018-12-011210.3389/fncel.2018.00502429693Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitroElvira Juzekaeva0Azat Gainutdinov1Marat Mukhtarov2Roustem Khazipov3Roustem Khazipov4Laboratory of Neurobiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, RussiaLaboratory of Neurobiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, RussiaLaboratory of Neurobiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, RussiaLaboratory of Neurobiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, RussiaAix Marseille Univ, INSERM, INMED, Marseille, FranceCerebral edema is a major, life threatening complication of ischemic brain damage. Previous studies using brain slices have revealed that cellular swelling and a concomitant increase in tissue transparency starts within minutes of the onset of metabolic insult in association with collective anoxic spreading depolarization (aSD). However, the dynamics of tissue swelling in brain slices under ischemia-like conditions remain elusive. Here, we explored the dynamics of brain tissue swelling induced by oxygen-glucose deprivation (OGD) in submerged rat barrel cortex slices. Video monitoring of the vertical and horizontal position of fluorescent dye-filled neurons and contrast slice surface imaging revealed elevation of the slice surface and a horizontal displacement of the cortical tissue during OGD. The OGD-induced tissue movement was also associated with an expansion of the slice borders. Tissue swelling started several minutes after aSD and continued during reperfusion with normal solution. Thirty minutes after aSD, slice borders had expanded by ~130 μm and the slice surface had moved up to attain a height of ~70 μm above control levels, which corresponded to a volume increase of ~30%. Hyperosmotic sucrose solution partially reduced the OGD-induced slice swelling. Thus, OGD-induced cortical slice tissue swelling in brain slices in vitro recapitulates many features of ischemic cerebral edema in vivo, its onset is tightly linked to aSD and it develops at a relatively slow pace after aSD. We propose that this model of cerebral edema in vitro could be useful for the exploration of the pathophysiological mechanisms underlying ischemic cerebral edema and in the search for an efficient treatment to this devastating condition.https://www.frontiersin.org/article/10.3389/fncel.2018.00502/fullbrain ischemiahypoxiaedemaswellingcortex |
| spellingShingle | Elvira Juzekaeva Azat Gainutdinov Marat Mukhtarov Roustem Khazipov Roustem Khazipov Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro Frontiers in Cellular Neuroscience brain ischemia hypoxia edema swelling cortex |
| title | Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro |
| title_full | Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro |
| title_fullStr | Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro |
| title_full_unstemmed | Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro |
| title_short | Dynamics of the Hypoxia—Induced Tissue Edema in the Rat Barrel Cortex in vitro |
| title_sort | dynamics of the hypoxia induced tissue edema in the rat barrel cortex in vitro |
| topic | brain ischemia hypoxia edema swelling cortex |
| url | https://www.frontiersin.org/article/10.3389/fncel.2018.00502/full |
| work_keys_str_mv | AT elvirajuzekaeva dynamicsofthehypoxiainducedtissueedemaintheratbarrelcortexinvitro AT azatgainutdinov dynamicsofthehypoxiainducedtissueedemaintheratbarrelcortexinvitro AT maratmukhtarov dynamicsofthehypoxiainducedtissueedemaintheratbarrelcortexinvitro AT roustemkhazipov dynamicsofthehypoxiainducedtissueedemaintheratbarrelcortexinvitro AT roustemkhazipov dynamicsofthehypoxiainducedtissueedemaintheratbarrelcortexinvitro |