Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem
An oxidative burst is an early response of plants to various biotic/abiotic stresses. In plant-microbe interactions, the plant body can induce oxidative burst to activate various defense mechanisms to combat phytopathogens. A localized oxidative burst is also one of the typical behaviors during hype...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/9/4812 |
| _version_ | 1797535485771382784 |
|---|---|
| author | Cunchun Yang W. G. Dilantha Fernando |
| author_facet | Cunchun Yang W. G. Dilantha Fernando |
| author_sort | Cunchun Yang |
| collection | DOAJ |
| description | An oxidative burst is an early response of plants to various biotic/abiotic stresses. In plant-microbe interactions, the plant body can induce oxidative burst to activate various defense mechanisms to combat phytopathogens. A localized oxidative burst is also one of the typical behaviors during hypersensitive response (HR) caused by gene-for-gene interaction. In this study, the occurrence of oxidative burst and its signaling pathways was studied from different levels of disease severity (i.e., susceptible, intermediate, and resistant) in the <i>B. napus</i>–<i>L. maculans</i> pathosystem. Canola cotyledons with distinct levels of resistance exhibited differential regulation of the genes involved in reactive oxygen species (ROS) accumulation and responses. Histochemical assays were carried out to understand the patterns of H<sub>2</sub>O<sub>2</sub> accumulation and cell death. Intermediate and resistant genotypes exhibited earlier accumulation of H<sub>2</sub>O<sub>2</sub> and emergence of cell death around the inoculation origins. The observations also suggested that the cotyledons with stronger resistance were able to form a protective region of intensive oxidative bursts between the areas with and without hyphal intrusions to block further fungal advancement to the uninfected regions. The qPCR analysis suggested that different onset patterns of some marker genes in ROS accumulation/programmed cell death (PCD) such as <i>RBOHD</i>, <i>MPK3</i> were associated with distinct levels of resistance from <i>B. napus</i> cultivars against <i>L. maculans</i>. The observations and datasets from this article indicated the distinct differences in ROS-related cellular behaviors and signaling between compatible and incompatible interactions. |
| first_indexed | 2024-03-10T11:45:19Z |
| format | Article |
| id | doaj.art-79673b7952ab4636b451be1bc83ea66c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:45:19Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-79673b7952ab4636b451be1bc83ea66c2023-11-21T18:06:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01229481210.3390/ijms22094812Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> PathosystemCunchun Yang0W. G. Dilantha Fernando1Department of Plant Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Plant Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaAn oxidative burst is an early response of plants to various biotic/abiotic stresses. In plant-microbe interactions, the plant body can induce oxidative burst to activate various defense mechanisms to combat phytopathogens. A localized oxidative burst is also one of the typical behaviors during hypersensitive response (HR) caused by gene-for-gene interaction. In this study, the occurrence of oxidative burst and its signaling pathways was studied from different levels of disease severity (i.e., susceptible, intermediate, and resistant) in the <i>B. napus</i>–<i>L. maculans</i> pathosystem. Canola cotyledons with distinct levels of resistance exhibited differential regulation of the genes involved in reactive oxygen species (ROS) accumulation and responses. Histochemical assays were carried out to understand the patterns of H<sub>2</sub>O<sub>2</sub> accumulation and cell death. Intermediate and resistant genotypes exhibited earlier accumulation of H<sub>2</sub>O<sub>2</sub> and emergence of cell death around the inoculation origins. The observations also suggested that the cotyledons with stronger resistance were able to form a protective region of intensive oxidative bursts between the areas with and without hyphal intrusions to block further fungal advancement to the uninfected regions. The qPCR analysis suggested that different onset patterns of some marker genes in ROS accumulation/programmed cell death (PCD) such as <i>RBOHD</i>, <i>MPK3</i> were associated with distinct levels of resistance from <i>B. napus</i> cultivars against <i>L. maculans</i>. The observations and datasets from this article indicated the distinct differences in ROS-related cellular behaviors and signaling between compatible and incompatible interactions.https://www.mdpi.com/1422-0067/22/9/4812<i>Leptosphaeria maculans</i><i>Brassica napus</i>reactive oxygen species (ROS)hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)programmed cell death (PCD)respiratory burst oxidase (RBOH) |
| spellingShingle | Cunchun Yang W. G. Dilantha Fernando Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem International Journal of Molecular Sciences <i>Leptosphaeria maculans</i> <i>Brassica napus</i> reactive oxygen species (ROS) hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) programmed cell death (PCD) respiratory burst oxidase (RBOH) |
| title | Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem |
| title_full | Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem |
| title_fullStr | Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem |
| title_full_unstemmed | Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem |
| title_short | Analysis of the Oxidative Burst and Its Relevant Signaling Pathways in <i>Leptosphaeria maculans—Brassica napus</i> Pathosystem |
| title_sort | analysis of the oxidative burst and its relevant signaling pathways in i leptosphaeria maculans brassica napus i pathosystem |
| topic | <i>Leptosphaeria maculans</i> <i>Brassica napus</i> reactive oxygen species (ROS) hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) programmed cell death (PCD) respiratory burst oxidase (RBOH) |
| url | https://www.mdpi.com/1422-0067/22/9/4812 |
| work_keys_str_mv | AT cunchunyang analysisoftheoxidativeburstanditsrelevantsignalingpathwaysinileptosphaeriamaculansbrassicanapusipathosystem AT wgdilanthafernando analysisoftheoxidativeburstanditsrelevantsignalingpathwaysinileptosphaeriamaculansbrassicanapusipathosystem |