Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes
Alkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/1/151 |
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| author | Kenta Nagahori Ning Qu Miyuki Kuramasu Yuki Ogawa Daisuke Kiyoshima Kaori Suyama Shogo Hayashi Kou Sakabe Takayuki Yoshimoto Masahiro Itoh |
| author_facet | Kenta Nagahori Ning Qu Miyuki Kuramasu Yuki Ogawa Daisuke Kiyoshima Kaori Suyama Shogo Hayashi Kou Sakabe Takayuki Yoshimoto Masahiro Itoh |
| author_sort | Kenta Nagahori |
| collection | DOAJ |
| description | Alkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important advances in the field of radiation oncology. At present, little is known about the relationship between the harmful effects and accumulated dose of irradiation derived from continuous low-dose radiation exposure. In this study, we examined the levels of mRNA transcripts encoding markers of 13 markers of germ cell differentiation and 28 Sertoli cell-specific products in single- and re-irradiated mice. Our results demonstrated that re-irradiation induced significantly decreased testicular weights with a significant decrease in germ cell differentiation mRNA species (<i>Spo11</i>, <i>Tnp1</i>, <i>Gfra1, Oct4, Sycp3, Ddx4</i>, <i>Boll, Crem, Prm1,</i> and <i>Acrosin</i>). In the 13 Sertoli cell-specific mRNA species decreased upon irradiation, six mRNA species (<i>Claudin-11,</i><i>Espn, Fshr, GATA1, Inhbb</i><i>,</i> and <i>Wt1</i>) showed significant differences between single- and re-irradiation. At the same time, different decreases in Sertoli cell-specific mRNA species were found in single-irradiation (<i>Aqp8</i>, <i>Clu</i>, <i>Cst12</i>, and <i>Wnt5a</i>) and re-irradiation (<i>Tjp1, occludin,</i><i>ZO</i>-1, and <i>ZO</i>-2) mice. These results indicate that long-term aspermatogenesis may differ after single- and re-irradiated treatment. |
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| last_indexed | 2024-03-10T01:24:53Z |
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| spelling | doaj.art-79688f5aab0741338a9aa718b35e26c32023-11-23T13:53:10ZengMDPI AGGenes2073-44252022-01-0113115110.3390/genes13010151Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse TestesKenta Nagahori0Ning Qu1Miyuki Kuramasu2Yuki Ogawa3Daisuke Kiyoshima4Kaori Suyama5Shogo Hayashi6Kou Sakabe7Takayuki Yoshimoto8Masahiro Itoh9Department of Anatomy, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa 259-1193, JapanDepartment of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa 259-1193, JapanDepartment of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa 259-1193, JapanDepartment of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, Kanagawa 259-1193, JapanImmuno Regulation, Institute of Medical Science, Tokyo Medical University, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, Tokyo 160-8402, JapanAlkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important advances in the field of radiation oncology. At present, little is known about the relationship between the harmful effects and accumulated dose of irradiation derived from continuous low-dose radiation exposure. In this study, we examined the levels of mRNA transcripts encoding markers of 13 markers of germ cell differentiation and 28 Sertoli cell-specific products in single- and re-irradiated mice. Our results demonstrated that re-irradiation induced significantly decreased testicular weights with a significant decrease in germ cell differentiation mRNA species (<i>Spo11</i>, <i>Tnp1</i>, <i>Gfra1, Oct4, Sycp3, Ddx4</i>, <i>Boll, Crem, Prm1,</i> and <i>Acrosin</i>). In the 13 Sertoli cell-specific mRNA species decreased upon irradiation, six mRNA species (<i>Claudin-11,</i><i>Espn, Fshr, GATA1, Inhbb</i><i>,</i> and <i>Wt1</i>) showed significant differences between single- and re-irradiation. At the same time, different decreases in Sertoli cell-specific mRNA species were found in single-irradiation (<i>Aqp8</i>, <i>Clu</i>, <i>Cst12</i>, and <i>Wnt5a</i>) and re-irradiation (<i>Tjp1, occludin,</i><i>ZO</i>-1, and <i>ZO</i>-2) mice. These results indicate that long-term aspermatogenesis may differ after single- and re-irradiated treatment.https://www.mdpi.com/2073-4425/13/1/151irradiationgerm cell differentiationSertoli tight junctionsanti-sperm antibody |
| spellingShingle | Kenta Nagahori Ning Qu Miyuki Kuramasu Yuki Ogawa Daisuke Kiyoshima Kaori Suyama Shogo Hayashi Kou Sakabe Takayuki Yoshimoto Masahiro Itoh Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes Genes irradiation germ cell differentiation Sertoli tight junctions anti-sperm antibody |
| title | Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes |
| title_full | Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes |
| title_fullStr | Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes |
| title_full_unstemmed | Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes |
| title_short | Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes |
| title_sort | changes in expression of specific mrna transcripts after single or re irradiation in mouse testes |
| topic | irradiation germ cell differentiation Sertoli tight junctions anti-sperm antibody |
| url | https://www.mdpi.com/2073-4425/13/1/151 |
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