Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture
Objectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures.Methods: Foreskin specimens from normal infants were collected immediately after circum...
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| Format: | Article |
| Language: | English |
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Sultan Qaboos University
2013-08-01
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| Series: | Sultan Qaboos University Medical Journal |
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| Online Access: | https://journals.squ.edu.om/index.php/squmj/article/view/1840 |
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| author | Fatma Al-Jasmi Thachillath Pramathan Adnan Swid Bahjat Sahari Harvey S. Penefsky Abdul-Kader Souid |
| author_facet | Fatma Al-Jasmi Thachillath Pramathan Adnan Swid Bahjat Sahari Harvey S. Penefsky Abdul-Kader Souid |
| author_sort | Fatma Al-Jasmi |
| collection | DOAJ |
| description | Objectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures.Methods: Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Results: In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confirming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confirming that the oxidation occurred mainly in the mitochondrial respiratory chain. The rate of foreskin respiration (mean ± SD) was 0.074 ± 0.02 μM O2 min-1 mg-1 (n = 23). The corresponding rate for fibroblast-rich cultures was 9.84 ± 2.43 μM O2 min-1 per 107 cells (n = 15). Fibroblast respiration was significantly lower in a male infant with dihydrolipoamide dehydrogenase gene mutations, but normalised with the addition of thiamine or carnitine. Conclusion: The foreskin and its fibroblast-rich culture are suitable for assessment of cellular respiration. However, the clinical utility of foreskin specimens to detect disorders of impaired cellular bioenergetics requires further investigation. |
| first_indexed | 2024-12-10T18:14:24Z |
| format | Article |
| id | doaj.art-796c28694c82469d986b2ccd7abdd0e3 |
| institution | Directory Open Access Journal |
| issn | 2075-051X 2075-0528 |
| language | English |
| last_indexed | 2024-12-10T18:14:24Z |
| publishDate | 2013-08-01 |
| publisher | Sultan Qaboos University |
| record_format | Article |
| series | Sultan Qaboos University Medical Journal |
| spelling | doaj.art-796c28694c82469d986b2ccd7abdd0e32022-12-22T01:38:23ZengSultan Qaboos UniversitySultan Qaboos University Medical Journal2075-051X2075-05282013-08-011334114161764Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich CultureFatma Al-Jasmi0Thachillath Pramathan1Adnan Swid2Bahjat Sahari3Harvey S. Penefsky4Abdul-Kader Souid5Department of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesTawam Hospital, Al Ain, United Arab EmiratesTawam Hospital, Al Ain, United Arab EmiratesFaculty of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Paediatrics, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesObjectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures.Methods: Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Results: In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confirming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confirming that the oxidation occurred mainly in the mitochondrial respiratory chain. The rate of foreskin respiration (mean ± SD) was 0.074 ± 0.02 μM O2 min-1 mg-1 (n = 23). The corresponding rate for fibroblast-rich cultures was 9.84 ± 2.43 μM O2 min-1 per 107 cells (n = 15). Fibroblast respiration was significantly lower in a male infant with dihydrolipoamide dehydrogenase gene mutations, but normalised with the addition of thiamine or carnitine. Conclusion: The foreskin and its fibroblast-rich culture are suitable for assessment of cellular respiration. However, the clinical utility of foreskin specimens to detect disorders of impaired cellular bioenergetics requires further investigation.https://journals.squ.edu.om/index.php/squmj/article/view/1840oxygenmitochondriaforeskinrespirationfibroblastsdihydrolipoamide dehydrogenasethiaminecarnitine |
| spellingShingle | Fatma Al-Jasmi Thachillath Pramathan Adnan Swid Bahjat Sahari Harvey S. Penefsky Abdul-Kader Souid Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture Sultan Qaboos University Medical Journal oxygen mitochondria foreskin respiration fibroblasts dihydrolipoamide dehydrogenase thiamine carnitine |
| title | Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture |
| title_full | Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture |
| title_fullStr | Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture |
| title_full_unstemmed | Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture |
| title_short | Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture |
| title_sort | mitochondrial oxygen consumption by the foreskin and its fibroblast rich culture |
| topic | oxygen mitochondria foreskin respiration fibroblasts dihydrolipoamide dehydrogenase thiamine carnitine |
| url | https://journals.squ.edu.om/index.php/squmj/article/view/1840 |
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