The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial
Idiopathic nephrotic syndrome (INS) is the most common cause of NS in children. It is characterized by the existence of edema, proteinuria, and hypoalbuminemia, as well as repeated relapses. The etiology remains unknown, but new evidence for its pathogenesis relates to the dysfunction of T-regulator...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
| Series: | Saudi Journal of Kidney Diseases and Transplantation |
| Online Access: | http://www.sjkdt.org/article.asp?issn=1319-2442;year=2022;volume=33;issue=8;spage=169;epage=178;aulast=Mohammed |
| _version_ | 1827779031143022592 |
|---|---|
| author | Rehab Ahmed Mohammed Sherin Khamis Hussein Sylvana Nady Gaber Ahmed Fatma Abonaga Walaa Abdelfattah Eman Sayed Said Amy Fakhry Boushra |
| author_facet | Rehab Ahmed Mohammed Sherin Khamis Hussein Sylvana Nady Gaber Ahmed Fatma Abonaga Walaa Abdelfattah Eman Sayed Said Amy Fakhry Boushra |
| author_sort | Rehab Ahmed Mohammed |
| collection | DOAJ |
| description | Idiopathic nephrotic syndrome (INS) is the most common cause of NS in children. It is characterized by the existence of edema, proteinuria, and hypoalbuminemia, as well as repeated relapses. The etiology remains unknown, but new evidence for its pathogenesis relates to the dysfunction of T-regulatory (T-reg) cells, which could be caused by dysbiosis of the gut microbiota. Our study aimed to investigate the effect of prebiotics and probiotics as adjuvant therapies for children with relapsing INS. The study was designed as a prospective open-label randomized clinical trial involving 30 children diagnosed with relapsing INS. The children were randomly divided into two groups. Group 1 was treated with prednisone only, and Group 2 was treated with prebiotics and probiotics in addition to prednisone. Fresh stool samples were collected from the children. Lactobacillus species were isolated and identified by conventional microbiological methods. The total number of Lactobacillus species was counted for each stool sample. The population of T-reg cells in the peripheral blood mononuclear cells was analyzed using flow cytometry. Children treated with prebiotics and probiotics in addition to steroids showed a significant increase in T-reg cells (CD4+/CD25+/FOXp3+) in the peripheral blood and a higher count of Lactobacillus species in their stool alongside a significant decrease in the rate of relapses in this group compared with Group 1. Treatment with prebiotics and probiotics signi-ficantly increased T-reg cells and decreased the rate of relapse in INS. |
| first_indexed | 2024-03-11T14:45:25Z |
| format | Article |
| id | doaj.art-796e1602b9cb4cc88967effd36d70b1c |
| institution | Directory Open Access Journal |
| issn | 1319-2442 |
| language | English |
| last_indexed | 2024-03-11T14:45:25Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Saudi Journal of Kidney Diseases and Transplantation |
| spelling | doaj.art-796e1602b9cb4cc88967effd36d70b1c2023-10-30T11:57:49ZengWolters Kluwer Medknow PublicationsSaudi Journal of Kidney Diseases and Transplantation1319-24422022-01-0133816917810.4103/1319-2442.384189The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical TrialRehab Ahmed MohammedSherin Khamis HusseinSylvana Nady GaberAhmedFatma AbonagaWalaa AbdelfattahEman Sayed SaidAmy Fakhry BoushraIdiopathic nephrotic syndrome (INS) is the most common cause of NS in children. It is characterized by the existence of edema, proteinuria, and hypoalbuminemia, as well as repeated relapses. The etiology remains unknown, but new evidence for its pathogenesis relates to the dysfunction of T-regulatory (T-reg) cells, which could be caused by dysbiosis of the gut microbiota. Our study aimed to investigate the effect of prebiotics and probiotics as adjuvant therapies for children with relapsing INS. The study was designed as a prospective open-label randomized clinical trial involving 30 children diagnosed with relapsing INS. The children were randomly divided into two groups. Group 1 was treated with prednisone only, and Group 2 was treated with prebiotics and probiotics in addition to prednisone. Fresh stool samples were collected from the children. Lactobacillus species were isolated and identified by conventional microbiological methods. The total number of Lactobacillus species was counted for each stool sample. The population of T-reg cells in the peripheral blood mononuclear cells was analyzed using flow cytometry. Children treated with prebiotics and probiotics in addition to steroids showed a significant increase in T-reg cells (CD4+/CD25+/FOXp3+) in the peripheral blood and a higher count of Lactobacillus species in their stool alongside a significant decrease in the rate of relapses in this group compared with Group 1. Treatment with prebiotics and probiotics signi-ficantly increased T-reg cells and decreased the rate of relapse in INS.http://www.sjkdt.org/article.asp?issn=1319-2442;year=2022;volume=33;issue=8;spage=169;epage=178;aulast=Mohammed |
| spellingShingle | Rehab Ahmed Mohammed Sherin Khamis Hussein Sylvana Nady Gaber Ahmed Fatma Abonaga Walaa Abdelfattah Eman Sayed Said Amy Fakhry Boushra The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial Saudi Journal of Kidney Diseases and Transplantation |
| title | The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial |
| title_full | The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial |
| title_fullStr | The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial |
| title_full_unstemmed | The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial |
| title_short | The Role of Prebiotics and Probiotics as an Adjuvant Therapy in Children with Idiopathic Relapsing Nephrotic Syndrome: A Prospective Open-label Clinical Trial |
| title_sort | role of prebiotics and probiotics as an adjuvant therapy in children with idiopathic relapsing nephrotic syndrome a prospective open label clinical trial |
| url | http://www.sjkdt.org/article.asp?issn=1319-2442;year=2022;volume=33;issue=8;spage=169;epage=178;aulast=Mohammed |
| work_keys_str_mv | AT rehabahmedmohammed theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT sherinkhamishussein theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT sylvananadygaber theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT ahmed theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT fatmaabonaga theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT walaaabdelfattah theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT emansayedsaid theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT amyfakhryboushra theroleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT rehabahmedmohammed roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT sherinkhamishussein roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT sylvananadygaber roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT ahmed roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT fatmaabonaga roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT walaaabdelfattah roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT emansayedsaid roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial AT amyfakhryboushra roleofprebioticsandprobioticsasanadjuvanttherapyinchildrenwithidiopathicrelapsingnephroticsyndromeaprospectiveopenlabelclinicaltrial |