Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i>
The appropriate level of dietary lipids is essential for the nutrient requirements, rapid growth, and health maintenance of aquatic animals, while excessive dietary lipid intake will lead to lipid deposition and affect fish health. However, the symptoms of excessive lipid deposition in the liver of...
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MDPI AG
2023-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1615 |
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| author | Miaomiao Xue Pao Xu Haibo Wen Jianxiang Chen Qingyong Wang Jiyan He Changchang He Changxin Kong Changyou Song Hongxia Li |
| author_facet | Miaomiao Xue Pao Xu Haibo Wen Jianxiang Chen Qingyong Wang Jiyan He Changchang He Changxin Kong Changyou Song Hongxia Li |
| author_sort | Miaomiao Xue |
| collection | DOAJ |
| description | The appropriate level of dietary lipids is essential for the nutrient requirements, rapid growth, and health maintenance of aquatic animals, while excessive dietary lipid intake will lead to lipid deposition and affect fish health. However, the symptoms of excessive lipid deposition in the liver of freshwater drums (<i>Aplodinotus grunniens</i>) remain unclear. In this study, a 4-month rearing experiment feeding with high-fat diets and a 6-week starvation stress experiment were conducted to evaluate the physiological alteration and underlying mechanism associated with lipid deposition in the liver of <i>A. grunniens</i>. From the results, high-fat-diet-induced lipid deposition was associated with increased condition factor (CF), viscerosomatic index (VSI), and hepatosomatic index (HSI). Meanwhile, lipid deposition led to physiological and metabolic disorders, inhibited antioxidant capacity, and exacerbated the burden of lipid metabolism. Lipid deposition promoted fatty acid synthesis but suppressed catabolism. Specifically, the transcriptome and metabolome showed significant enrichment of lipid metabolism and antioxidant pathways. In addition, the interaction analysis suggested that peroxisome proliferator-activated receptor (<i>PPAR</i>)-mediated 13-S-hydroxyoctadecenoic acid (13 (s)-HODE) could serve as the key target in regulating lipid metabolism and oxidative stress during lipid deposition in <i>A. grunniens</i>. Inversely, with a lipid intake restriction experiment, <i>PPARs</i> were confirmed to regulate lipid expenditure and physiological homeostasis in <i>A. grunniens</i>. These results uncover the molecular basis of and provide specific molecular targets for fatty liver control and prevention, which are of great importance for the sustainable development of <i>A. grunniens</i>. |
| first_indexed | 2024-03-11T00:10:37Z |
| format | Article |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:37Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7977260f5b754a8dbf0da45605ec43372023-11-19T00:02:46ZengMDPI AGAntioxidants2076-39212023-08-01128161510.3390/antiox12081615Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i>Miaomiao Xue0Pao Xu1Haibo Wen2Jianxiang Chen3Qingyong Wang4Jiyan He5Changchang He6Changxin Kong7Changyou Song8Hongxia Li9Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaThe appropriate level of dietary lipids is essential for the nutrient requirements, rapid growth, and health maintenance of aquatic animals, while excessive dietary lipid intake will lead to lipid deposition and affect fish health. However, the symptoms of excessive lipid deposition in the liver of freshwater drums (<i>Aplodinotus grunniens</i>) remain unclear. In this study, a 4-month rearing experiment feeding with high-fat diets and a 6-week starvation stress experiment were conducted to evaluate the physiological alteration and underlying mechanism associated with lipid deposition in the liver of <i>A. grunniens</i>. From the results, high-fat-diet-induced lipid deposition was associated with increased condition factor (CF), viscerosomatic index (VSI), and hepatosomatic index (HSI). Meanwhile, lipid deposition led to physiological and metabolic disorders, inhibited antioxidant capacity, and exacerbated the burden of lipid metabolism. Lipid deposition promoted fatty acid synthesis but suppressed catabolism. Specifically, the transcriptome and metabolome showed significant enrichment of lipid metabolism and antioxidant pathways. In addition, the interaction analysis suggested that peroxisome proliferator-activated receptor (<i>PPAR</i>)-mediated 13-S-hydroxyoctadecenoic acid (13 (s)-HODE) could serve as the key target in regulating lipid metabolism and oxidative stress during lipid deposition in <i>A. grunniens</i>. Inversely, with a lipid intake restriction experiment, <i>PPARs</i> were confirmed to regulate lipid expenditure and physiological homeostasis in <i>A. grunniens</i>. These results uncover the molecular basis of and provide specific molecular targets for fatty liver control and prevention, which are of great importance for the sustainable development of <i>A. grunniens</i>.https://www.mdpi.com/2076-3921/12/8/1615<i>Aplodinotus grunniens</i>high-fat dietstarvation stresslipid depositionoxidative stress<i>PPAR</i> signaling |
| spellingShingle | Miaomiao Xue Pao Xu Haibo Wen Jianxiang Chen Qingyong Wang Jiyan He Changchang He Changxin Kong Changyou Song Hongxia Li Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> Antioxidants <i>Aplodinotus grunniens</i> high-fat diet starvation stress lipid deposition oxidative stress <i>PPAR</i> signaling |
| title | Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> |
| title_full | Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> |
| title_fullStr | Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> |
| title_full_unstemmed | Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> |
| title_short | Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, <i>Aplodinotus grunniens</i> |
| title_sort | peroxisome proliferator activated receptor signaling mediated 13 s hydroxyoctadecenoic acid is involved in lipid metabolic disorder and oxidative stress in the liver of freshwater drum i aplodinotus grunniens i |
| topic | <i>Aplodinotus grunniens</i> high-fat diet starvation stress lipid deposition oxidative stress <i>PPAR</i> signaling |
| url | https://www.mdpi.com/2076-3921/12/8/1615 |
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