Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma
Global efforts have been undertaken to define the genome‐wide distribution of epigenetic markers in cancerous tissues, which provide an invaluable opportunity to understand cancer biology and identify predictive signatures. Several studies have focused on the gene expression patterns of squamous car...
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| Format: | Article |
| Language: | English |
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Wiley
2020-08-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12912 |
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| author | Jingrun Yang Kuixia Xie Zihui Wang Chengxin Li |
| author_facet | Jingrun Yang Kuixia Xie Zihui Wang Chengxin Li |
| author_sort | Jingrun Yang |
| collection | DOAJ |
| description | Global efforts have been undertaken to define the genome‐wide distribution of epigenetic markers in cancerous tissues, which provide an invaluable opportunity to understand cancer biology and identify predictive signatures. Several studies have focused on the gene expression patterns of squamous carcinoma to identify tumor subtypes and find prognostic and therapeutic targets because squamous carcinoma genomes showed high instability. However, the number of reliable reports referring prognostic significance of genes and their role in squamous carcinoma is still quite limited. Krüppel‐like factor 7 (KLF7) is a transcription factor that is widely expressed in numerous human tissues at low levels. Members of the KLF family have established roles in tumor cell fate, stress response, cell survival and the tumor‐initiating properties of cancer stem‐like cells. Hence to investigate whether KFL7 expression from cancer tissue holds promise as a prognostic and/or therapeutic target, we analyzed gene expression profiles from squamous carcinoma and surgical margin tissues in The Cancer Genome Atlas. We identified significant up‐regulation of KLF7 in squamous carcinoma, which was confirmed by immunohistochemical staining. Elevated KLF7 expression was associated with poor squamous carcinoma prognosis before and after correcting for confounding factors by multivariate Cox regression analysis. Several pathways, such as Neurotrophin and GnRH pathways, were activated in KLF7‐up‐regulated squamous carcinoma samples through Gene Set Enrichment Analysis. In conclusion, we consolidate the potential role(s) of KLF7 in squamous carcinoma carcinogenesis from The Cancer Genome Atlas surgical margin tissue, offering insights into expression signatures that are potentially useful for prognosis modalities. |
| first_indexed | 2024-12-12T13:18:19Z |
| format | Article |
| id | doaj.art-7978285deb9548eca8c7f22f6c06b7fa |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-12T13:18:19Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-7978285deb9548eca8c7f22f6c06b7fa2022-12-22T00:23:22ZengWileyFEBS Open Bio2211-54632020-08-011081577158610.1002/2211-5463.12912Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinomaJingrun Yang0Kuixia Xie1Zihui Wang2Chengxin Li3Department of Dermatology PLA General Hospital Beijing ChinaDermatological Department Tianjin Fifth Centre Hospital Tianjin ChinaDepartment of Pharmacy Beijing Chao‐Yang Hospital Capital Medical University Beijing ChinaDepartment of Dermatology PLA General Hospital Beijing ChinaGlobal efforts have been undertaken to define the genome‐wide distribution of epigenetic markers in cancerous tissues, which provide an invaluable opportunity to understand cancer biology and identify predictive signatures. Several studies have focused on the gene expression patterns of squamous carcinoma to identify tumor subtypes and find prognostic and therapeutic targets because squamous carcinoma genomes showed high instability. However, the number of reliable reports referring prognostic significance of genes and their role in squamous carcinoma is still quite limited. Krüppel‐like factor 7 (KLF7) is a transcription factor that is widely expressed in numerous human tissues at low levels. Members of the KLF family have established roles in tumor cell fate, stress response, cell survival and the tumor‐initiating properties of cancer stem‐like cells. Hence to investigate whether KFL7 expression from cancer tissue holds promise as a prognostic and/or therapeutic target, we analyzed gene expression profiles from squamous carcinoma and surgical margin tissues in The Cancer Genome Atlas. We identified significant up‐regulation of KLF7 in squamous carcinoma, which was confirmed by immunohistochemical staining. Elevated KLF7 expression was associated with poor squamous carcinoma prognosis before and after correcting for confounding factors by multivariate Cox regression analysis. Several pathways, such as Neurotrophin and GnRH pathways, were activated in KLF7‐up‐regulated squamous carcinoma samples through Gene Set Enrichment Analysis. In conclusion, we consolidate the potential role(s) of KLF7 in squamous carcinoma carcinogenesis from The Cancer Genome Atlas surgical margin tissue, offering insights into expression signatures that are potentially useful for prognosis modalities.https://doi.org/10.1002/2211-5463.12912carcinogenesisimmunohistochemical stainingKLF7prognosissquamous carcinomaTCGA |
| spellingShingle | Jingrun Yang Kuixia Xie Zihui Wang Chengxin Li Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma FEBS Open Bio carcinogenesis immunohistochemical staining KLF7 prognosis squamous carcinoma TCGA |
| title | Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| title_full | Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| title_fullStr | Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| title_full_unstemmed | Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| title_short | Elevated KLF7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| title_sort | elevated klf7 levels may serve as a prognostic signature and might contribute to progression of squamous carcinoma |
| topic | carcinogenesis immunohistochemical staining KLF7 prognosis squamous carcinoma TCGA |
| url | https://doi.org/10.1002/2211-5463.12912 |
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