Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition.
It has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0286809 |
| _version_ | 1797394415607611392 |
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| author | Mozhgan Hashemi Ahmad Moieni Mohammad Sadegh Sabet |
| author_facet | Mozhgan Hashemi Ahmad Moieni Mohammad Sadegh Sabet |
| author_sort | Mozhgan Hashemi |
| collection | DOAJ |
| description | It has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant microspore culture when we mixed casein hydrolysate (0 and 100 mg L-1) with four amino acids: proline (0, 100, 500, and 900 mg L-1), glutamine (0 and 800 mg L-1), serine (0 and 100 mg L-1), and alanine (0 and 100 mg L-1). The results showed that a combination of 800 mg L-1 of glutamine, 100 mg L-1 of serine, 100 mg L-1 of casein hydrolysate, and 500 mg L-1 of proline produced the maximum number of calli per Petri dish (938). Calli had a globular shape and a compact appearance when formed in media containing 500 mg L-1 of proline (alone or combined with serine, alanine, and/or casein hydrolysate). Most of these structures were observed in a medium with 500 mg L-1 of proline, 100 mg L-1 of casein hydrolysate, and 100 mg L-1 of serine. We also investigated what happened when gum arabic (2400, 2600, 3600, 4600, and 5600 mg L-1) was combined with proline (0 and 500 mg L-1), casein hydrolysate (0 and 100 mg L-1), and glutamine (0, 400, and 800 mg L-1). The findings demonstrated the involvement of proline in the increase of calli. Overall, the results give us new information about how amino acids work in eggplant microspore culture and suggest that proline can move this plant's microspore androgenesis pathway forward. |
| first_indexed | 2024-03-09T00:19:22Z |
| format | Article |
| id | doaj.art-7979279a03d243f1819efd245e5417b0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-09T00:19:22Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7979279a03d243f1819efd245e5417b02023-12-12T05:36:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01186e028680910.1371/journal.pone.0286809Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition.Mozhgan HashemiAhmad MoieniMohammad Sadegh SabetIt has been proposed that the composition of the culture medium, especially its amino acids, is an important part of getting microspore androgenesis to occur in some plants. However, there have been far fewer studies done on the Solanaceae family. In this study, we studied what happened to eggplant microspore culture when we mixed casein hydrolysate (0 and 100 mg L-1) with four amino acids: proline (0, 100, 500, and 900 mg L-1), glutamine (0 and 800 mg L-1), serine (0 and 100 mg L-1), and alanine (0 and 100 mg L-1). The results showed that a combination of 800 mg L-1 of glutamine, 100 mg L-1 of serine, 100 mg L-1 of casein hydrolysate, and 500 mg L-1 of proline produced the maximum number of calli per Petri dish (938). Calli had a globular shape and a compact appearance when formed in media containing 500 mg L-1 of proline (alone or combined with serine, alanine, and/or casein hydrolysate). Most of these structures were observed in a medium with 500 mg L-1 of proline, 100 mg L-1 of casein hydrolysate, and 100 mg L-1 of serine. We also investigated what happened when gum arabic (2400, 2600, 3600, 4600, and 5600 mg L-1) was combined with proline (0 and 500 mg L-1), casein hydrolysate (0 and 100 mg L-1), and glutamine (0, 400, and 800 mg L-1). The findings demonstrated the involvement of proline in the increase of calli. Overall, the results give us new information about how amino acids work in eggplant microspore culture and suggest that proline can move this plant's microspore androgenesis pathway forward.https://doi.org/10.1371/journal.pone.0286809 |
| spellingShingle | Mozhgan Hashemi Ahmad Moieni Mohammad Sadegh Sabet Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. PLoS ONE |
| title | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. |
| title_full | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. |
| title_fullStr | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. |
| title_full_unstemmed | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. |
| title_short | Improving the isolated microspore culture in eggplant (Solanum melongena L.) with amino acid nutrition. |
| title_sort | improving the isolated microspore culture in eggplant solanum melongena l with amino acid nutrition |
| url | https://doi.org/10.1371/journal.pone.0286809 |
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