Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study
Gastric cancer (GC) is a common lethal malignancy worldwide. Gastroscopy is an effective screening technique for decreasing mortality. However, there are still limited useful non-invasive markers for early detection of GC. Bile acids are important molecules for the modulation of energy metabolism. W...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1039786/full |
| _version_ | 1811220284011708416 |
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| author | Chen Pan Chen Pan Chen Pan Dawei Deng Dawei Deng Dawei Deng Tianfu Wei Zeming Wu Biao Zhang Qihang Yuan Guogang Liang Yanfeng Liu Peiyuan Yin Peiyuan Yin |
| author_facet | Chen Pan Chen Pan Chen Pan Dawei Deng Dawei Deng Dawei Deng Tianfu Wei Zeming Wu Biao Zhang Qihang Yuan Guogang Liang Yanfeng Liu Peiyuan Yin Peiyuan Yin |
| author_sort | Chen Pan |
| collection | DOAJ |
| description | Gastric cancer (GC) is a common lethal malignancy worldwide. Gastroscopy is an effective screening technique for decreasing mortality. However, there are still limited useful non-invasive markers for early detection of GC. Bile acids are important molecules for the modulation of energy metabolism. With an in-depth targeted method for accurate quantitation of 80 bile acids (BAs), we aimed to find potential biomarkers for the early screening of GC. A cohort with 280 participants was enrolled, including 113 GC, 22 benign gastric lesions (BGL) and 145 healthy controls. Potential markers were identified using a random forest machine algorithm in the discovery cohort (n=180), then validated in an internal validation cohort (n=78) and a group with 22 BGL. The results represented significant alterations in the circulating BA pool between GC and the controls. BAs also exhibited significant correlations with various clinical traits. Then, we developed a diagnostic panel that comprised six BAs or ratios for GC detection. The panel showed high accuracy for the diagnosis of GC with AUC of 1 (95%CI: 1.00-1.00) and 0.98 (95%CI: 0.93-1.00) in the discovery and validation cohort, respectively. This 6-BAs panel was also able to identify early GC with AUC of 1 (95%CI: 0.999-1.00) and 0.94 (95%CI: 0.83-1.00) in the discovery and validation cohort, respectively. Meanwhile, this panel achieved a good differential diagnosis between GC and BGL and the AUC was 0.873 (95%CI: 0.812-0.934). The alternations of serum bile acids are characteristic metabolic features of GC. Bile acids could be promising biomarkers for the early diagnosis of GC. |
| first_indexed | 2024-04-12T07:38:48Z |
| format | Article |
| id | doaj.art-7979fe43909749528228388b42cab77d |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-12T07:38:48Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-7979fe43909749528228388b42cab77d2022-12-22T03:41:51ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-11-011310.3389/fendo.2022.10397861039786Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control studyChen Pan0Chen Pan1Chen Pan2Dawei Deng3Dawei Deng4Dawei Deng5Tianfu Wei6Zeming Wu7Biao Zhang8Qihang Yuan9Guogang Liang10Yanfeng Liu11Peiyuan Yin12Peiyuan Yin13Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaClinical Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of General Surgery, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaClinical Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Hepato-Biliary-Pancreas, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaiPhenome Biotechnology (Yun Pu Kang) Inc., Dalian, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaClinical Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, Dalian, ChinaInstitute of Integrative Medicine, Dalian Medical University, Dalian, ChinaGastric cancer (GC) is a common lethal malignancy worldwide. Gastroscopy is an effective screening technique for decreasing mortality. However, there are still limited useful non-invasive markers for early detection of GC. Bile acids are important molecules for the modulation of energy metabolism. With an in-depth targeted method for accurate quantitation of 80 bile acids (BAs), we aimed to find potential biomarkers for the early screening of GC. A cohort with 280 participants was enrolled, including 113 GC, 22 benign gastric lesions (BGL) and 145 healthy controls. Potential markers were identified using a random forest machine algorithm in the discovery cohort (n=180), then validated in an internal validation cohort (n=78) and a group with 22 BGL. The results represented significant alterations in the circulating BA pool between GC and the controls. BAs also exhibited significant correlations with various clinical traits. Then, we developed a diagnostic panel that comprised six BAs or ratios for GC detection. The panel showed high accuracy for the diagnosis of GC with AUC of 1 (95%CI: 1.00-1.00) and 0.98 (95%CI: 0.93-1.00) in the discovery and validation cohort, respectively. This 6-BAs panel was also able to identify early GC with AUC of 1 (95%CI: 0.999-1.00) and 0.94 (95%CI: 0.83-1.00) in the discovery and validation cohort, respectively. Meanwhile, this panel achieved a good differential diagnosis between GC and BGL and the AUC was 0.873 (95%CI: 0.812-0.934). The alternations of serum bile acids are characteristic metabolic features of GC. Bile acids could be promising biomarkers for the early diagnosis of GC.https://www.frontiersin.org/articles/10.3389/fendo.2022.1039786/fullgastric cancerbile acidsmetabolomicsbiomarkersLC MS |
| spellingShingle | Chen Pan Chen Pan Chen Pan Dawei Deng Dawei Deng Dawei Deng Tianfu Wei Zeming Wu Biao Zhang Qihang Yuan Guogang Liang Yanfeng Liu Peiyuan Yin Peiyuan Yin Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study Frontiers in Endocrinology gastric cancer bile acids metabolomics biomarkers LC MS |
| title | Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study |
| title_full | Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study |
| title_fullStr | Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study |
| title_full_unstemmed | Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study |
| title_short | Metabolomics study identified bile acids as potential biomarkers for gastric cancer: A case control study |
| title_sort | metabolomics study identified bile acids as potential biomarkers for gastric cancer a case control study |
| topic | gastric cancer bile acids metabolomics biomarkers LC MS |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1039786/full |
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