Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel
A fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect ind...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/12/29824 |
| _version_ | 1798004855241965568 |
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| author | Ping-Hsien Chiu Chen-Hsun Weng Ruey-Jen Yang |
| author_facet | Ping-Hsien Chiu Chen-Hsun Weng Ruey-Jen Yang |
| author_sort | Ping-Hsien Chiu |
| collection | DOAJ |
| description | A fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect induced at the micro/nano interface under the application of an external electric field, while species separation is achieved by exploiting the different electrophoretic mobilities of the sample components. The experimental results show that the device is capable of detecting C-reactive protein (CRP) with an initial concentration as low as 9.50 × 10−6 mg/L given a sufficient preconcentration time and driving voltage. In addition, it is shown that a mixed-species sample consisting of three negatively-charged components (bovine serum albumin (BSA), tetramethylrhodamine(TAMRA) isothiocyanate-Dextran and fluorescent polymer beads) can be separated and preconcentrated within 20 min given a driving voltage of 100 V across 1 cm microchannel in length. In general, the present results confirm the feasibility of the device for the immunoassay or detection of various multi-species samples under low concentration in the biochemical and biomedical fields. The novel device can therefore improve the detection limit of traditional medical facilities. |
| first_indexed | 2024-04-11T12:30:12Z |
| format | Article |
| id | doaj.art-797a26efe45042c8b15db29389637a3c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:30:12Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-797a26efe45042c8b15db29389637a3c2022-12-22T04:23:47ZengMDPI AGSensors1424-82202015-12-011512307043071510.3390/s151229824s151229824Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent MicrochannelPing-Hsien Chiu0Chen-Hsun Weng1Ruey-Jen Yang2Department of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanMedical Device Innovation Center, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanA fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect induced at the micro/nano interface under the application of an external electric field, while species separation is achieved by exploiting the different electrophoretic mobilities of the sample components. The experimental results show that the device is capable of detecting C-reactive protein (CRP) with an initial concentration as low as 9.50 × 10−6 mg/L given a sufficient preconcentration time and driving voltage. In addition, it is shown that a mixed-species sample consisting of three negatively-charged components (bovine serum albumin (BSA), tetramethylrhodamine(TAMRA) isothiocyanate-Dextran and fluorescent polymer beads) can be separated and preconcentrated within 20 min given a driving voltage of 100 V across 1 cm microchannel in length. In general, the present results confirm the feasibility of the device for the immunoassay or detection of various multi-species samples under low concentration in the biochemical and biomedical fields. The novel device can therefore improve the detection limit of traditional medical facilities.http://www.mdpi.com/1424-8220/15/12/29824microfluidicspreconcentrationion concentration polarizationelectrophoresis |
| spellingShingle | Ping-Hsien Chiu Chen-Hsun Weng Ruey-Jen Yang Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel Sensors microfluidics preconcentration ion concentration polarization electrophoresis |
| title | Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel |
| title_full | Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel |
| title_fullStr | Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel |
| title_full_unstemmed | Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel |
| title_short | Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel |
| title_sort | preconcentration and separation of mixed species samples near a nano junction in a convergent microchannel |
| topic | microfluidics preconcentration ion concentration polarization electrophoresis |
| url | http://www.mdpi.com/1424-8220/15/12/29824 |
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