Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS
Objective To explore the effect and mechanism of pinocembrin on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods AGS cells were divided into different test groups according to concentrations of pinocembrin(50, 100, 200, 400 μmol/L), NC group, 200 μmol/L pinocembr...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-11-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-11-1744.pdf |
| _version_ | 1827390593759707136 |
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| author | LI Xun, FU Xiao-xia, FAN Li-wei, WANG Jing-kun, ZHANG Dong-jiao, ZHAO Yan-huan, ZHANG Lei, ZHAO Yu-hong |
| author_facet | LI Xun, FU Xiao-xia, FAN Li-wei, WANG Jing-kun, ZHANG Dong-jiao, ZHAO Yan-huan, ZHANG Lei, ZHAO Yu-hong |
| author_sort | LI Xun, FU Xiao-xia, FAN Li-wei, WANG Jing-kun, ZHANG Dong-jiao, ZHAO Yan-huan, ZHANG Lei, ZHAO Yu-hong |
| collection | DOAJ |
| description | Objective To explore the effect and mechanism of pinocembrin on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods AGS cells were divided into different test groups according to concentrations of pinocembrin(50, 100, 200, 400 μmol/L), NC group, 200 μmol/L pinocembrin + anti-miR-NC group, 200 μmol/L pinocembrin + anti-miR-34a-5p group. The proliferation, apoptosis, migration, invasion ability and protein expression of AGS cells were detected by CCK-8 assay, flow cytometry, Transwell chamber assay and Western blot. The expression of miR-34a-5p was detected by real-time fluorescent quantitative PCR (RT-qPCR). Results Different concentrations of pinocembrin reduced the proliferation activity of AGS cells (P＜0.05) and the proportion of in G0/G1 phase cells was increased while the proportion of S phase cells decreased. The expressions of MMP2, MMP9, p-PI3K and p-AKT protein decreased. The cell migration and invasion were inhibited. The expression of miR-34a-5p was increased (P＜0.05), which reduced the inhibitory effect of pinocembrin on the prolifera- tion, migration and invasion of AGS cells. Conclusions Pinocembrin can inhibit the proliferation, migration and invasion of AGS cells by improving the expression of miR-34a-5p. |
| first_indexed | 2024-03-08T16:54:59Z |
| format | Article |
| id | doaj.art-7980b9bf3232450088ec3fa5a03beff3 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:59Z |
| publishDate | 2022-11-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-7980b9bf3232450088ec3fa5a03beff32024-01-05T02:36:38ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-11-0142111744175010.16352/j.issn.1001-6325.2022.11.1744Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGSLI Xun, FU Xiao-xia, FAN Li-wei, WANG Jing-kun, ZHANG Dong-jiao, ZHAO Yan-huan, ZHANG Lei, ZHAO Yu-hong0Department of Gastroenterology, the 82nd Army Hospital of the Chinese People's Liberation Army, Baoding 071000, ChinaObjective To explore the effect and mechanism of pinocembrin on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods AGS cells were divided into different test groups according to concentrations of pinocembrin(50, 100, 200, 400 μmol/L), NC group, 200 μmol/L pinocembrin + anti-miR-NC group, 200 μmol/L pinocembrin + anti-miR-34a-5p group. The proliferation, apoptosis, migration, invasion ability and protein expression of AGS cells were detected by CCK-8 assay, flow cytometry, Transwell chamber assay and Western blot. The expression of miR-34a-5p was detected by real-time fluorescent quantitative PCR (RT-qPCR). Results Different concentrations of pinocembrin reduced the proliferation activity of AGS cells (P＜0.05) and the proportion of in G0/G1 phase cells was increased while the proportion of S phase cells decreased. The expressions of MMP2, MMP9, p-PI3K and p-AKT protein decreased. The cell migration and invasion were inhibited. The expression of miR-34a-5p was increased (P＜0.05), which reduced the inhibitory effect of pinocembrin on the prolifera- tion, migration and invasion of AGS cells. Conclusions Pinocembrin can inhibit the proliferation, migration and invasion of AGS cells by improving the expression of miR-34a-5p.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-11-1744.pdfpinocembrin|mir-34a-5p|proliferation|migration|invasion |
| spellingShingle | LI Xun, FU Xiao-xia, FAN Li-wei, WANG Jing-kun, ZHANG Dong-jiao, ZHAO Yan-huan, ZHANG Lei, ZHAO Yu-hong Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS Jichu yixue yu linchuang pinocembrin|mir-34a-5p|proliferation|migration|invasion |
| title | Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS |
| title_full | Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS |
| title_fullStr | Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS |
| title_full_unstemmed | Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS |
| title_short | Pinocembrin inhibits the proliferation, migration and invasion of gastric cancer cell line AGS |
| title_sort | pinocembrin inhibits the proliferation migration and invasion of gastric cancer cell line ags |
| topic | pinocembrin|mir-34a-5p|proliferation|migration|invasion |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-11-1744.pdf |
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