First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019.
Uganda established a domestic Viral Hemorrhagic Fever (VHF) testing capacity in 2010 in response to the increasing occurrence of filovirus outbreaks. In July 2018, the neighboring Democratic Republic of Congo (DRC) experienced its 10th Ebola Virus Disease (EVD) outbreak and for the duration of the o...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-02-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010205 |
| _version_ | 1818272447468666880 |
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| author | Luke Nyakarahuka Sophia Mulei Shannon Whitmer Kyondo Jackson Alex Tumusiime Amy Schuh Jimmy Baluku Allison Joyce Felix Ocom Jayne B Tusiime Joel M Montgomery Stephen Balinandi Julius J Lutwama John D Klena Trevor R Shoemaker ‘Kasese EVD Outbreak Response Team’ |
| author_facet | Luke Nyakarahuka Sophia Mulei Shannon Whitmer Kyondo Jackson Alex Tumusiime Amy Schuh Jimmy Baluku Allison Joyce Felix Ocom Jayne B Tusiime Joel M Montgomery Stephen Balinandi Julius J Lutwama John D Klena Trevor R Shoemaker ‘Kasese EVD Outbreak Response Team’ |
| author_sort | Luke Nyakarahuka |
| collection | DOAJ |
| description | Uganda established a domestic Viral Hemorrhagic Fever (VHF) testing capacity in 2010 in response to the increasing occurrence of filovirus outbreaks. In July 2018, the neighboring Democratic Republic of Congo (DRC) experienced its 10th Ebola Virus Disease (EVD) outbreak and for the duration of the outbreak, the Ugandan Ministry of Health (MOH) initiated a national EVD preparedness stance. Almost one year later, on 10th June 2019, three family members who had contracted EVD in the DRC crossed into Uganda to seek medical treatment. Samples were collected from all the suspected cases using internationally established biosafety protocols and submitted for VHF diagnostic testing at Uganda Virus Research Institute. All samples were initially tested by RT-PCR for ebolaviruses, marburgviruses, Rift Valley fever (RVF) virus and Crimean-Congo hemorrhagic fever (CCHF) virus. Four people were identified as being positive for Zaire ebolavirus, marking the first report of Zaire ebolavirus in Uganda. In-country Next Generation Sequencing (NGS) and phylogenetic analysis was performed for the first time in Uganda, confirming the outbreak as imported from DRC at two different time point from different clades. This rapid response by the MoH, UVRI and partners led to the control of the outbreak and prevention of secondary virus transmission. |
| first_indexed | 2024-12-12T21:42:13Z |
| format | Article |
| id | doaj.art-79820f3b50844ae1871e602f31f3627e |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-12T21:42:13Z |
| publishDate | 2022-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-79820f3b50844ae1871e602f31f3627e2022-12-22T00:11:00ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-02-01162e001020510.1371/journal.pntd.0010205First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019.Luke NyakarahukaSophia MuleiShannon WhitmerKyondo JacksonAlex TumusiimeAmy SchuhJimmy BalukuAllison JoyceFelix OcomJayne B TusiimeJoel M MontgomeryStephen BalinandiJulius J LutwamaJohn D KlenaTrevor R Shoemaker‘Kasese EVD Outbreak Response Team’Uganda established a domestic Viral Hemorrhagic Fever (VHF) testing capacity in 2010 in response to the increasing occurrence of filovirus outbreaks. In July 2018, the neighboring Democratic Republic of Congo (DRC) experienced its 10th Ebola Virus Disease (EVD) outbreak and for the duration of the outbreak, the Ugandan Ministry of Health (MOH) initiated a national EVD preparedness stance. Almost one year later, on 10th June 2019, three family members who had contracted EVD in the DRC crossed into Uganda to seek medical treatment. Samples were collected from all the suspected cases using internationally established biosafety protocols and submitted for VHF diagnostic testing at Uganda Virus Research Institute. All samples were initially tested by RT-PCR for ebolaviruses, marburgviruses, Rift Valley fever (RVF) virus and Crimean-Congo hemorrhagic fever (CCHF) virus. Four people were identified as being positive for Zaire ebolavirus, marking the first report of Zaire ebolavirus in Uganda. In-country Next Generation Sequencing (NGS) and phylogenetic analysis was performed for the first time in Uganda, confirming the outbreak as imported from DRC at two different time point from different clades. This rapid response by the MoH, UVRI and partners led to the control of the outbreak and prevention of secondary virus transmission.https://doi.org/10.1371/journal.pntd.0010205 |
| spellingShingle | Luke Nyakarahuka Sophia Mulei Shannon Whitmer Kyondo Jackson Alex Tumusiime Amy Schuh Jimmy Baluku Allison Joyce Felix Ocom Jayne B Tusiime Joel M Montgomery Stephen Balinandi Julius J Lutwama John D Klena Trevor R Shoemaker ‘Kasese EVD Outbreak Response Team’ First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. PLoS Neglected Tropical Diseases |
| title | First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. |
| title_full | First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. |
| title_fullStr | First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. |
| title_full_unstemmed | First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. |
| title_short | First laboratory confirmation and sequencing of Zaire ebolavirus in Uganda following two independent introductions of cases from the 10th Ebola Outbreak in the Democratic Republic of the Congo, June 2019. |
| title_sort | first laboratory confirmation and sequencing of zaire ebolavirus in uganda following two independent introductions of cases from the 10th ebola outbreak in the democratic republic of the congo june 2019 |
| url | https://doi.org/10.1371/journal.pntd.0010205 |
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