Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS
A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified β-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatmen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520340773 |
| _version_ | 1831543557158076416 |
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| author | Jan Lembcke Uta Ceglarek Georg Martin Fiedler Sven Baumann Alexander Leichtle Joachim Thiery |
| author_facet | Jan Lembcke Uta Ceglarek Georg Martin Fiedler Sven Baumann Alexander Leichtle Joachim Thiery |
| author_sort | Jan Lembcke |
| collection | DOAJ |
| description | A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified β-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from ∼3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols ranged between 0.25 and 0.68 μg/l. Linear ranges were between 1 and 1,000 μg/l. The within-run and between-run variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS.Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum. |
| first_indexed | 2024-12-17T00:53:13Z |
| format | Article |
| id | doaj.art-798577d8a50b46848194af70e23441db |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T00:53:13Z |
| publishDate | 2005-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-798577d8a50b46848194af70e23441db2022-12-21T22:09:42ZengElsevierJournal of Lipid Research0022-22752005-01-014612126Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MSJan Lembcke0Uta Ceglarek1Georg Martin Fiedler2Sven Baumann3Alexander Leichtle4Joachim Thiery5Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyInstitute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, GermanyA novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified β-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from ∼3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols ranged between 0.25 and 0.68 μg/l. Linear ranges were between 1 and 1,000 μg/l. The within-run and between-run variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS.Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum.http://www.sciencedirect.com/science/article/pii/S0022227520340773free phytosterolsphytosterol estersliquid chromatographyplant sterols |
| spellingShingle | Jan Lembcke Uta Ceglarek Georg Martin Fiedler Sven Baumann Alexander Leichtle Joachim Thiery Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS Journal of Lipid Research free phytosterols phytosterol esters liquid chromatography plant sterols |
| title | Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
| title_full | Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
| title_fullStr | Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
| title_full_unstemmed | Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
| title_short | Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS |
| title_sort | rapid quantification of free and esterified phytosterols in human serum using appi lc ms ms |
| topic | free phytosterols phytosterol esters liquid chromatography plant sterols |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520340773 |
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