Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials
Sugarcane is an important sugar and energy crop in the world. Germplasm innovation is a significant way to breed breakthrough sugarcane varieties. Modern sugarcane varieties all contain the blood relationship of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i>. Hi...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/4/819 |
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| author | Xueting Li Yirong Guo Fei Huang Qiusong Wang Jin Chai Fan Yu Jiayun Wu Muqing Zhang Zuhu Deng |
| author_facet | Xueting Li Yirong Guo Fei Huang Qiusong Wang Jin Chai Fan Yu Jiayun Wu Muqing Zhang Zuhu Deng |
| author_sort | Xueting Li |
| collection | DOAJ |
| description | Sugarcane is an important sugar and energy crop in the world. Germplasm innovation is a significant way to breed breakthrough sugarcane varieties. Modern sugarcane varieties all contain the blood relationship of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i>. High sugar results from <i>S. officinarum</i> and the resistance genes from <i>S. spontaneum</i>. In order to improve the sugarcane quality, breeders use <i>S. officinarum</i> and <i>S. spontaneum</i> to cross and obtain hybrid offspring with high sugar and high resistance. Therefore, the authenticity of <i>S. officinarum</i> and <i>S. spontaneum</i> progeny materials directly affects the efficiency of sugarcane breeding. In this study, the tetra-primer amplification hindered mutation system (ARMS PCR) was used to identify ten suspected <i>S. officinarum</i> and eleven suspected <i>S. spontaneum</i> germplasm materials, then further validated by chromosome counting and genome in situ hybridization (GISH). Among the ten suspected <i>S. officinarum</i> materials to be identified, three were real <i>S. officinarum</i> materials, they were 14NG124, 51NG103, and Guan A. Nine of the eleven suspected <i>S. spontaneum</i> to be identified were fake <i>S. spontaneum</i> materials, these were Yunge 2007-12-165, Guangxi 87-20, Yunnan 82-16, Yunge 2007-11, YNLC 16, Laos No. 2, Yunnan 82-29, 2015-83, and 2013-20. The ARMS PCR results were the same as the GISH results. The three real <i>S. officinarum</i> materials had 80 chromosomes. Using ARMS PCR and GISH, three <i>S. officinarum</i> and nine <i>S. spontaneum</i> materials were proven to be authentic. Through chromosome number statistics, it was found that the three real <i>S. officinarum</i> had 80 chromosomes. Authentic materials were identified and selected to enrich the genetic background of sugarcane through hybridization and reduce the influence on the breeding process of the misuse of fake <i>S. officinarum</i> and <i>S. spontaneum</i>. |
| first_indexed | 2024-03-09T11:18:17Z |
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| last_indexed | 2024-03-09T11:18:17Z |
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| spelling | doaj.art-79872fe34e5044d984ed5143685835f72023-12-01T00:26:27ZengMDPI AGAgronomy2073-43952022-03-0112481910.3390/agronomy12040819Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm MaterialsXueting Li0Yirong Guo1Fei Huang2Qiusong Wang3Jin Chai4Fan Yu5Jiayun Wu6Muqing Zhang7Zuhu Deng8National Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaGuangdong Sugarcane Genetic Improvement Engineering Center, Institute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaState Key Laboratory for Protection and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, ChinaNational Engineering Research Center for Sugarcane & Guangxi Key Laboratory of Sugarcane Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSugarcane is an important sugar and energy crop in the world. Germplasm innovation is a significant way to breed breakthrough sugarcane varieties. Modern sugarcane varieties all contain the blood relationship of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i>. High sugar results from <i>S. officinarum</i> and the resistance genes from <i>S. spontaneum</i>. In order to improve the sugarcane quality, breeders use <i>S. officinarum</i> and <i>S. spontaneum</i> to cross and obtain hybrid offspring with high sugar and high resistance. Therefore, the authenticity of <i>S. officinarum</i> and <i>S. spontaneum</i> progeny materials directly affects the efficiency of sugarcane breeding. In this study, the tetra-primer amplification hindered mutation system (ARMS PCR) was used to identify ten suspected <i>S. officinarum</i> and eleven suspected <i>S. spontaneum</i> germplasm materials, then further validated by chromosome counting and genome in situ hybridization (GISH). Among the ten suspected <i>S. officinarum</i> materials to be identified, three were real <i>S. officinarum</i> materials, they were 14NG124, 51NG103, and Guan A. Nine of the eleven suspected <i>S. spontaneum</i> to be identified were fake <i>S. spontaneum</i> materials, these were Yunge 2007-12-165, Guangxi 87-20, Yunnan 82-16, Yunge 2007-11, YNLC 16, Laos No. 2, Yunnan 82-29, 2015-83, and 2013-20. The ARMS PCR results were the same as the GISH results. The three real <i>S. officinarum</i> materials had 80 chromosomes. Using ARMS PCR and GISH, three <i>S. officinarum</i> and nine <i>S. spontaneum</i> materials were proven to be authentic. Through chromosome number statistics, it was found that the three real <i>S. officinarum</i> had 80 chromosomes. Authentic materials were identified and selected to enrich the genetic background of sugarcane through hybridization and reduce the influence on the breeding process of the misuse of fake <i>S. officinarum</i> and <i>S. spontaneum</i>.https://www.mdpi.com/2073-4395/12/4/819<i>S. officinarum</i><i>S. spontaneum</i>tetra-primer ARMS PCRin situ hybridizationgermplasm resources |
| spellingShingle | Xueting Li Yirong Guo Fei Huang Qiusong Wang Jin Chai Fan Yu Jiayun Wu Muqing Zhang Zuhu Deng Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials Agronomy <i>S. officinarum</i> <i>S. spontaneum</i> tetra-primer ARMS PCR in situ hybridization germplasm resources |
| title | Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials |
| title_full | Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials |
| title_fullStr | Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials |
| title_full_unstemmed | Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials |
| title_short | Authenticity Identification of <i>Saccharum officinarum</i> and <i>Saccharum spontaneum</i> Germplasm Materials |
| title_sort | authenticity identification of i saccharum officinarum i and i saccharum spontaneum i germplasm materials |
| topic | <i>S. officinarum</i> <i>S. spontaneum</i> tetra-primer ARMS PCR in situ hybridization germplasm resources |
| url | https://www.mdpi.com/2073-4395/12/4/819 |
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