Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses
Apis mellifera royal jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. As a glandular product, RJ has been shown to contain a substantial number of extracellul...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253123000288 |
| _version_ | 1797893779614597120 |
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| author | Simón Álvarez Pamina Contreras-Kallens Sebastian Aguayo Orlando Ramírez Catalina Vallejos Jorge Ruiz Eva Carrasco-Gallardo Stefanie Troncoso-Vera Bernardo Morales Christina M.A.P. Schuh |
| author_facet | Simón Álvarez Pamina Contreras-Kallens Sebastian Aguayo Orlando Ramírez Catalina Vallejos Jorge Ruiz Eva Carrasco-Gallardo Stefanie Troncoso-Vera Bernardo Morales Christina M.A.P. Schuh |
| author_sort | Simón Álvarez |
| collection | DOAJ |
| description | Apis mellifera royal jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs), and, in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing-associated effects. Molecular analysis of RJEVs verified the presence of exosomal markers such as CD63 and syntenin, and cargo molecules MRJP1, defensin-1, and jellein-3. Furthermore, RJEVs were demonstrated to modulate mesenchymal stem cell (MSC) differentiation and secretome, as well as decrease LPS-induced inflammation in macrophages by blocking the mitogen-activated protein kinase (MAPK) pathway. In vivo studies confirmed antibacterial effects of RJEVs and demonstrated an acceleration of wound healing in a splinted mouse model. This study suggests that RJEVs play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing. Transfer of RJ into the clinics has been impeded by the high complexity of the raw material. Isolating EVs from the raw RJ decreases the complexity while allowing standardization and quality control, bringing a natural nano-therapy one step closer to the clinics. |
| first_indexed | 2024-04-10T06:59:32Z |
| format | Article |
| id | doaj.art-798dd39777be48edb0b648efdb2796d1 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-04-10T06:59:32Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-798dd39777be48edb0b648efdb2796d12023-02-28T04:08:38ZengElsevierMolecular Therapy: Nucleic Acids2162-25312023-03-0131541552Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responsesSimón Álvarez0Pamina Contreras-Kallens1Sebastian Aguayo2Orlando Ramírez3Catalina Vallejos4Jorge Ruiz5Eva Carrasco-Gallardo6Stefanie Troncoso-Vera7Bernardo Morales8Christina M.A.P. Schuh9Centro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, ChileCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, ChileDentistry School, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile; Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, ChileCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, ChileLaboratorio de Medicina Experimental, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, ChileLaboratorio de Medicina Experimental, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, ChileLaboratorio de Histopatología, Cemesi, Santiago, ChileLaboratorio de Histopatología, Cemesi, Santiago, ChileFacultad de Salud, Universidad del Alba, Santiago, ChileCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile; Corresponding author. Christina Schuh, Clínica Alemana-Universidad del Desarrollo, Avenida Plaza 680, Las Condes, Santiago de Chile.Apis mellifera royal jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs), and, in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing-associated effects. Molecular analysis of RJEVs verified the presence of exosomal markers such as CD63 and syntenin, and cargo molecules MRJP1, defensin-1, and jellein-3. Furthermore, RJEVs were demonstrated to modulate mesenchymal stem cell (MSC) differentiation and secretome, as well as decrease LPS-induced inflammation in macrophages by blocking the mitogen-activated protein kinase (MAPK) pathway. In vivo studies confirmed antibacterial effects of RJEVs and demonstrated an acceleration of wound healing in a splinted mouse model. This study suggests that RJEVs play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing. Transfer of RJ into the clinics has been impeded by the high complexity of the raw material. Isolating EVs from the raw RJ decreases the complexity while allowing standardization and quality control, bringing a natural nano-therapy one step closer to the clinics.http://www.sciencedirect.com/science/article/pii/S2162253123000288MT: Special Issue - Exploiting Extracellular Vesicles as Therapeutic Agentsinterkingdom communicationanti-inflammatory extracellular vesiclesnano-therapydefensin-1extracellular vesicle delivery |
| spellingShingle | Simón Álvarez Pamina Contreras-Kallens Sebastian Aguayo Orlando Ramírez Catalina Vallejos Jorge Ruiz Eva Carrasco-Gallardo Stefanie Troncoso-Vera Bernardo Morales Christina M.A.P. Schuh Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses Molecular Therapy: Nucleic Acids MT: Special Issue - Exploiting Extracellular Vesicles as Therapeutic Agents interkingdom communication anti-inflammatory extracellular vesicles nano-therapy defensin-1 extracellular vesicle delivery |
| title | Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| title_full | Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| title_fullStr | Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| title_full_unstemmed | Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| title_short | Royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| title_sort | royal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses |
| topic | MT: Special Issue - Exploiting Extracellular Vesicles as Therapeutic Agents interkingdom communication anti-inflammatory extracellular vesicles nano-therapy defensin-1 extracellular vesicle delivery |
| url | http://www.sciencedirect.com/science/article/pii/S2162253123000288 |
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