Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico

This paper aimed to evaluate the contamination with mycotoxigenic fungi and total aflatoxins in stored corn from different sites in Puebla and Tlaxcala, Mexico. Methodology. The study was conducted at two sites in Puebla (San Salvador El Seco and Junta Auxiliar La Resurrección) and two sites in Tlax...

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Main Author: K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-Pérez
Format: Article
Language:English
Published: Technoscience Publications 2024-03-01
Series:Nature Environment and Pollution Technology
Subjects:
Online Access:https://neptjournal.com/upload-images/(56)D-1512.pdf
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author K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-Pérez
author_facet K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-Pérez
author_sort K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-Pérez
collection DOAJ
description This paper aimed to evaluate the contamination with mycotoxigenic fungi and total aflatoxins in stored corn from different sites in Puebla and Tlaxcala, Mexico. Methodology. The study was conducted at two sites in Puebla (San Salvador El Seco and Junta Auxiliar La Resurrección) and two sites in Tlaxcala (Tlaltepango and Nativitas). A total of 80 samples of stored corn were collected. Identification of Aspergillus flavus was performed by microculture techniques and specific taxonomic keys (macromorphological and micromorphological). Then, samples of contaminated corn were selected, and aflatoxin production was confirmed using a direct solid-phase ELISA kit. A total of 25 A. flavus strains were identified. Other possible mycotoxin-producing fungi were Penicillium (n=52) and Fusarium (n=19). Regarding total aflatoxin contamination, all samples were contaminated within a range of 1.589 to 11.854 μg/kg, and the average concentration was 6.3 μg/kg corn. Implications. The detection of mycotoxigenic fungi in the samples tested and of aflatoxins in corn highlights the importance of monitoring these fungi. Since food safety is at risk, it shows the need for methods to control these fungi and their metabolites.
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spelling doaj.art-798ee0421a964b7a8900c166736785692024-03-12T16:37:29ZengTechnoscience PublicationsNature Environment and Pollution Technology0972-62682395-34542024-03-0123158358910.46488/NEPT.2024.v23i01.056Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, MexicoK. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-PérezThis paper aimed to evaluate the contamination with mycotoxigenic fungi and total aflatoxins in stored corn from different sites in Puebla and Tlaxcala, Mexico. Methodology. The study was conducted at two sites in Puebla (San Salvador El Seco and Junta Auxiliar La Resurrección) and two sites in Tlaxcala (Tlaltepango and Nativitas). A total of 80 samples of stored corn were collected. Identification of Aspergillus flavus was performed by microculture techniques and specific taxonomic keys (macromorphological and micromorphological). Then, samples of contaminated corn were selected, and aflatoxin production was confirmed using a direct solid-phase ELISA kit. A total of 25 A. flavus strains were identified. Other possible mycotoxin-producing fungi were Penicillium (n=52) and Fusarium (n=19). Regarding total aflatoxin contamination, all samples were contaminated within a range of 1.589 to 11.854 μg/kg, and the average concentration was 6.3 μg/kg corn. Implications. The detection of mycotoxigenic fungi in the samples tested and of aflatoxins in corn highlights the importance of monitoring these fungi. Since food safety is at risk, it shows the need for methods to control these fungi and their metabolites.https://neptjournal.com/upload-images/(56)D-1512.pdfmycotoxigenic fungi, aspergillus flavus, aflatoxins, corn kernel
spellingShingle K. Saez-Gomez, R. Avila-Sosa, M. Huerta-Lara, F. Avelino-Flores and R. Munguia-Pérez
Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
Nature Environment and Pollution Technology
mycotoxigenic fungi, aspergillus flavus, aflatoxins, corn kernel
title Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
title_full Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
title_fullStr Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
title_full_unstemmed Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
title_short Determination of Mycotoxigenic Fungi and Total Aflatoxins in Stored Corn from Sites of Puebla and Tlaxcala, Mexico
title_sort determination of mycotoxigenic fungi and total aflatoxins in stored corn from sites of puebla and tlaxcala mexico
topic mycotoxigenic fungi, aspergillus flavus, aflatoxins, corn kernel
url https://neptjournal.com/upload-images/(56)D-1512.pdf
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