Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses
The goal of this study was to investigate differences in tear peptidome/proteome profiles of aqueous-deficient dry eye, Meibomian gland dysfunction and control individuals. Tears from 93 individuals were collected by capillary and subjected to solid-phase extraction followed by MALDI-TOF for profili...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | EuPA Open Proteomics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221296851400021X |
| _version_ | 1818064160438616064 |
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| author | Nerea González Ibon Iloro Javier Soria Juan A. Duran Alaitz Santamaría Felix Elortza Tatiana Suárez |
| author_facet | Nerea González Ibon Iloro Javier Soria Juan A. Duran Alaitz Santamaría Felix Elortza Tatiana Suárez |
| author_sort | Nerea González |
| collection | DOAJ |
| description | The goal of this study was to investigate differences in tear peptidome/proteome profiles of aqueous-deficient dry eye, Meibomian gland dysfunction and control individuals. Tears from 93 individuals were collected by capillary and subjected to solid-phase extraction followed by MALDI-TOF for profiling analysis.
Obtained spectra were aligned by variable penalty dynamic time warping (VPdtw) and the resulting data analyzed using multivariate statistics. Comparative analyses revealed good performance of VPdtw and a high discrimination of groups with a correct assignment of 89.3% using twelve informative peaks. SDS–PAGE followed by MALDI-TOF/TOF analysis allowed identification of lipocalin-1 as a biomarker candidate. |
| first_indexed | 2024-12-10T14:31:35Z |
| format | Article |
| id | doaj.art-798fd35d8739417ba5079edd107c0f31 |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-10T14:31:35Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-798fd35d8739417ba5079edd107c0f312022-12-22T01:44:55ZengElsevierEuPA Open Proteomics2212-96852014-06-013C20621510.1016/j.euprot.2014.02.016Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analysesNerea González0Ibon Iloro1Javier Soria2Juan A. Duran3Alaitz Santamaría4Felix Elortza5Tatiana Suárez6Bioftalmik Applied Research, Science and Technology Park of Bizkaia, Ed. 800, 2nd Floor, E-48160 Derio, Bizkaia, SpainProteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII, Science and Technology Park of Bizkaia, 48160 Derio, Bizkaia, SpainBioftalmik Applied Research, Science and Technology Park of Bizkaia, Ed. 800, 2nd Floor, E-48160 Derio, Bizkaia, SpainInstituto Clínico Quirúrgico de Oftalmología (ICQO), Virgen de Begoña N° 34, E-48006 Bilbao, Bizkaia, SpainDepartment of Ophthalmology, Cruces Hospital, Plaza Cruces 12, E-48903 Baracaldo, Bizkaia, SpainProteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII, Science and Technology Park of Bizkaia, 48160 Derio, Bizkaia, SpainBioftalmik Applied Research, Science and Technology Park of Bizkaia, Ed. 800, 2nd Floor, E-48160 Derio, Bizkaia, SpainThe goal of this study was to investigate differences in tear peptidome/proteome profiles of aqueous-deficient dry eye, Meibomian gland dysfunction and control individuals. Tears from 93 individuals were collected by capillary and subjected to solid-phase extraction followed by MALDI-TOF for profiling analysis. Obtained spectra were aligned by variable penalty dynamic time warping (VPdtw) and the resulting data analyzed using multivariate statistics. Comparative analyses revealed good performance of VPdtw and a high discrimination of groups with a correct assignment of 89.3% using twelve informative peaks. SDS–PAGE followed by MALDI-TOF/TOF analysis allowed identification of lipocalin-1 as a biomarker candidate.http://www.sciencedirect.com/science/article/pii/S221296851400021XDry eyeMeibomian gland dysfunctionTearsMALDI-TOFProfilingWarpingPeptidomics |
| spellingShingle | Nerea González Ibon Iloro Javier Soria Juan A. Duran Alaitz Santamaría Felix Elortza Tatiana Suárez Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses EuPA Open Proteomics Dry eye Meibomian gland dysfunction Tears MALDI-TOF Profiling Warping Peptidomics |
| title | Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses |
| title_full | Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses |
| title_fullStr | Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses |
| title_full_unstemmed | Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses |
| title_short | Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses |
| title_sort | human tear peptide protein profiling study of ocular surface diseases by spe maldi tof mass spectrometry analyses |
| topic | Dry eye Meibomian gland dysfunction Tears MALDI-TOF Profiling Warping Peptidomics |
| url | http://www.sciencedirect.com/science/article/pii/S221296851400021X |
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