Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis
We previously reported on a 3-hydroxy fatty acid that is secreted via cryptococcal capsular protuberances - possibly to promote pathogenesis and survival. Thus, we investigated the role of this molecule in mediating the fate of Cryptococcus (C.) neoformans and the related species C. gattii when pred...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01351/full |
| _version_ | 1817975581730406400 |
|---|---|
| author | Uju Lynda Madu Adepemi O Ogundeji Bonang M Mochochoko Carolina H Pohl Jacobus eAlbertyn Chantel W Swart J William Allwood Andrew D Southam Warwick B Dunn Robin C May Robin C May Olihile M Sebolai Olihile M Sebolai |
| author_facet | Uju Lynda Madu Adepemi O Ogundeji Bonang M Mochochoko Carolina H Pohl Jacobus eAlbertyn Chantel W Swart J William Allwood Andrew D Southam Warwick B Dunn Robin C May Robin C May Olihile M Sebolai Olihile M Sebolai |
| author_sort | Uju Lynda Madu |
| collection | DOAJ |
| description | We previously reported on a 3-hydroxy fatty acid that is secreted via cryptococcal capsular protuberances - possibly to promote pathogenesis and survival. Thus, we investigated the role of this molecule in mediating the fate of Cryptococcus (C.) neoformans and the related species C. gattii when predated upon by amoebae. We show that this molecule protects cells against the phagocytic effects of amoebae. C. neoformans UOFS Y-1378 (which produces 3-hydroxy fatty acids) was less sensitive towards amoebae compared to C. neoformans LMPE 046 and C. gattii R265 (both do not produce 3-hydroxy fatty acids) and addition of 3-hydroxy fatty acids to C. neoformans LMPE 046 and C. gattii R265 culture media, causes these strains to become more resistant to amoebal predation. Conversely, addition of aspirin (a 3-hydroxy fatty acid inhibitor) to C. neoformans UOFS Y-1378 culture media made cells more susceptible to amoebae. Our data suggest that this molecule is secreted at a high enough concentration to effect intracellular signalling within amoeba, which in turn, promotes fungal survival. |
| first_indexed | 2024-04-13T21:51:50Z |
| format | Article |
| id | doaj.art-799126e257f846c698a96512a910a070 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T21:51:50Z |
| publishDate | 2015-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-799126e257f846c698a96512a910a0702022-12-22T02:28:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-12-01610.3389/fmicb.2015.01351164619Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosisUju Lynda Madu0Adepemi O Ogundeji1Bonang M Mochochoko2Carolina H Pohl3Jacobus eAlbertyn4Chantel W Swart5J William Allwood6Andrew D Southam7Warwick B Dunn8Robin C May9Robin C May10Olihile M Sebolai11Olihile M Sebolai12University of the Free StateUniversity of the Free StateUniversity of the Free StateUniversity of the Free StateUniversity of the Free StateUniversity of the Free StateUniversity of BirminghamUniversity of BirminghamUniversity of BirminghamUniversity of BirminghamUniversity of BirminghamUniversity of the Free StateUniversity of BirminghamWe previously reported on a 3-hydroxy fatty acid that is secreted via cryptococcal capsular protuberances - possibly to promote pathogenesis and survival. Thus, we investigated the role of this molecule in mediating the fate of Cryptococcus (C.) neoformans and the related species C. gattii when predated upon by amoebae. We show that this molecule protects cells against the phagocytic effects of amoebae. C. neoformans UOFS Y-1378 (which produces 3-hydroxy fatty acids) was less sensitive towards amoebae compared to C. neoformans LMPE 046 and C. gattii R265 (both do not produce 3-hydroxy fatty acids) and addition of 3-hydroxy fatty acids to C. neoformans LMPE 046 and C. gattii R265 culture media, causes these strains to become more resistant to amoebal predation. Conversely, addition of aspirin (a 3-hydroxy fatty acid inhibitor) to C. neoformans UOFS Y-1378 culture media made cells more susceptible to amoebae. Our data suggest that this molecule is secreted at a high enough concentration to effect intracellular signalling within amoeba, which in turn, promotes fungal survival.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01351/fullAmoebaCryptococcusPhagocytosisprotection3-Hydroxy fatty acids |
| spellingShingle | Uju Lynda Madu Adepemi O Ogundeji Bonang M Mochochoko Carolina H Pohl Jacobus eAlbertyn Chantel W Swart J William Allwood Andrew D Southam Warwick B Dunn Robin C May Robin C May Olihile M Sebolai Olihile M Sebolai Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis Frontiers in Microbiology Amoeba Cryptococcus Phagocytosis protection 3-Hydroxy fatty acids |
| title | Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis |
| title_full | Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis |
| title_fullStr | Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis |
| title_full_unstemmed | Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis |
| title_short | Cryptococcal 3-hydroxy fatty acids protect cells against amoebal phagocytosis |
| title_sort | cryptococcal 3 hydroxy fatty acids protect cells against amoebal phagocytosis |
| topic | Amoeba Cryptococcus Phagocytosis protection 3-Hydroxy fatty acids |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01351/full |
| work_keys_str_mv | AT ujulyndamadu cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT adepemioogundeji cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT bonangmmochochoko cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT carolinahpohl cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT jacobusealbertyn cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT chantelwswart cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT jwilliamallwood cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT andrewdsoutham cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT warwickbdunn cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT robincmay cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT robincmay cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT olihilemsebolai cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis AT olihilemsebolai cryptococcal3hydroxyfattyacidsprotectcellsagainstamoebalphagocytosis |