circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer
Circular RNAs (circRNAs) are a novel type of endogenous non-coding RNAs (ncRNA). Many studies showed that circRNAs played different biological functions in triple-negative breast cancer (TNBC). However, the potential molecular mechanism of circRNAs in TNBC still remains to be investigated. In this s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022039421 |
| _version_ | 1811173417987080192 |
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| author | Hao Xu Mengdie Fang Bowen Zuo Wanxin Yao Juan Ren Yanmei Zhang |
| author_facet | Hao Xu Mengdie Fang Bowen Zuo Wanxin Yao Juan Ren Yanmei Zhang |
| author_sort | Hao Xu |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) are a novel type of endogenous non-coding RNAs (ncRNA). Many studies showed that circRNAs played different biological functions in triple-negative breast cancer (TNBC). However, the potential molecular mechanism of circRNAs in TNBC still remains to be investigated. In this study, circAR-E2E4 was defined as a novel circRNA involved in TNBC progression, derived from and regulated by androgen receptor (AR). CCK-8 assay showed circAR-E2E4 regulated TNBC cell proliferation. Potential binding miRNAs of circAR-E2E4 were predicted and miR-665 was identified to have a great prognosis value. Three databases were employed to predict target genes of miR-665, and STAT3 was regarded as the most potential downstream genes analyzed by protein-protein interaction (PPI), hub gene screening, correlation analysis, and survival analysis. Finally, knockdown of circAR-E2E4 led to the decrease of STAT3 expression. Collectively, the regulatory network circAR-E2E4-miR-665-STAT3 axis we constructed was associated with TNBC progression, providing a promising diagnostic, prognostic, and therapeutic target in future treatment for TNBC. |
| first_indexed | 2024-04-10T17:46:31Z |
| format | Article |
| id | doaj.art-79926111690947949219f0a20eb6a749 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:46:31Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-79926111690947949219f0a20eb6a7492023-02-03T04:58:27ZengElsevierHeliyon2405-84402023-01-0191e12654circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancerHao Xu0Mengdie Fang1Bowen Zuo2Wanxin Yao3Juan Ren4Yanmei Zhang5School of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, China; Department of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaSchool of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, ChinaSchool of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, ChinaSchool of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, ChinaSchool of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, ChinaSchool of Laboratory Medicine and Bioengineering, Hangzhou Medical College, Hangzhou 310013, China; Corresponding author.Circular RNAs (circRNAs) are a novel type of endogenous non-coding RNAs (ncRNA). Many studies showed that circRNAs played different biological functions in triple-negative breast cancer (TNBC). However, the potential molecular mechanism of circRNAs in TNBC still remains to be investigated. In this study, circAR-E2E4 was defined as a novel circRNA involved in TNBC progression, derived from and regulated by androgen receptor (AR). CCK-8 assay showed circAR-E2E4 regulated TNBC cell proliferation. Potential binding miRNAs of circAR-E2E4 were predicted and miR-665 was identified to have a great prognosis value. Three databases were employed to predict target genes of miR-665, and STAT3 was regarded as the most potential downstream genes analyzed by protein-protein interaction (PPI), hub gene screening, correlation analysis, and survival analysis. Finally, knockdown of circAR-E2E4 led to the decrease of STAT3 expression. Collectively, the regulatory network circAR-E2E4-miR-665-STAT3 axis we constructed was associated with TNBC progression, providing a promising diagnostic, prognostic, and therapeutic target in future treatment for TNBC.http://www.sciencedirect.com/science/article/pii/S2405844022039421Circular RNAmicroRNAAndrogen receptorSTAT3Triple-negative breast cancer |
| spellingShingle | Hao Xu Mengdie Fang Bowen Zuo Wanxin Yao Juan Ren Yanmei Zhang circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer Heliyon Circular RNA microRNA Androgen receptor STAT3 Triple-negative breast cancer |
| title | circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer |
| title_full | circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer |
| title_fullStr | circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer |
| title_full_unstemmed | circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer |
| title_short | circAR-E2E4-miR-665-STAT3 axis is a potential regulatory network in triple-negative breast cancer |
| title_sort | circar e2e4 mir 665 stat3 axis is a potential regulatory network in triple negative breast cancer |
| topic | Circular RNA microRNA Androgen receptor STAT3 Triple-negative breast cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022039421 |
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