Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach
Summary: Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is a heteromeric complex composed of a ligand-specific receptor and the co-receptor (ORco). In this study, we identified the ORco gene of the cockroach, Periplane...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222005429 |
| _version_ | 1811255360249397248 |
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| author | Kosuke Tateishi Takayuki Watanabe Hiroshi Nishino Makoto Mizunami Hidehiro Watanabe |
| author_facet | Kosuke Tateishi Takayuki Watanabe Hiroshi Nishino Makoto Mizunami Hidehiro Watanabe |
| author_sort | Kosuke Tateishi |
| collection | DOAJ |
| description | Summary: Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is a heteromeric complex composed of a ligand-specific receptor and the co-receptor (ORco). In this study, we identified the ORco gene of the cockroach, Periplaneta americana (PameORco), and performed RNAi-based functional analysis of PameORco. All OSNs in the basiconic sensilla expressed PameORco and received a large variety of odors including sex pheromones. In trichoid sensilla, a PameORco-positive OSN was consistently paired with a PameORco-negative OSN tuned to acids. In adult cockroaches injected with PameORco dsRNA at the nymphal stage, the expression of PameORco, odor receptions via ORs, and its central processing were strongly suppressed. These results provide new insights into the molecular basis of olfactory reception in the cockroach. The long-lasting and irreversible effects of PameORco RNAi would be an effective method for controlling the household pest. |
| first_indexed | 2024-04-12T17:22:36Z |
| format | Article |
| id | doaj.art-7993077bde3c4546885e2816042c0185 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T17:22:36Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-7993077bde3c4546885e2816042c01852022-12-22T03:23:25ZengElsevieriScience2589-00422022-05-01255104272Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroachKosuke Tateishi0Takayuki Watanabe1Hiroshi Nishino2Makoto Mizunami3Hidehiro Watanabe4Department of Earth System Science, Fukuoka University, Fukuoka 814-0180, JapanResearch Center for Integrative Evolutionary Science, School of Advanced Sciences, Sokendai-Hayama, Shonan Village, Hayama 240-0193, Japan; Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanResearch Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, JapanFaculty of Science, Hokkaido University, Sapporo 060-0810, JapanDepartment of Earth System Science, Fukuoka University, Fukuoka 814-0180, Japan; Corresponding authorSummary: Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is a heteromeric complex composed of a ligand-specific receptor and the co-receptor (ORco). In this study, we identified the ORco gene of the cockroach, Periplaneta americana (PameORco), and performed RNAi-based functional analysis of PameORco. All OSNs in the basiconic sensilla expressed PameORco and received a large variety of odors including sex pheromones. In trichoid sensilla, a PameORco-positive OSN was consistently paired with a PameORco-negative OSN tuned to acids. In adult cockroaches injected with PameORco dsRNA at the nymphal stage, the expression of PameORco, odor receptions via ORs, and its central processing were strongly suppressed. These results provide new insights into the molecular basis of olfactory reception in the cockroach. The long-lasting and irreversible effects of PameORco RNAi would be an effective method for controlling the household pest.http://www.sciencedirect.com/science/article/pii/S2589004222005429EntomologyMolecular neuroscienceSensory neuroscience |
| spellingShingle | Kosuke Tateishi Takayuki Watanabe Hiroshi Nishino Makoto Mizunami Hidehiro Watanabe Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach iScience Entomology Molecular neuroscience Sensory neuroscience |
| title | Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach |
| title_full | Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach |
| title_fullStr | Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach |
| title_full_unstemmed | Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach |
| title_short | Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach |
| title_sort | silencing the odorant receptor co receptor impairs olfactory reception in a sensillum specific manner in the cockroach |
| topic | Entomology Molecular neuroscience Sensory neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222005429 |
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