Intra-uterine exposure of horses to Sarcocystis spp. antigens

The aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum sa...

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Main Authors: A.M. Antonello, G.C. Cadore, F.L. Pivoto, G. Camillo, P. Braunig, L.A. Sangioni, E. Pompermayer, L.F.P. Gondim, F.S.F. Vogel
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2016-04-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200271&lng=en&tlng=en
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author A.M. Antonello
G.C. Cadore
F.L. Pivoto
G. Camillo
P. Braunig
L.A. Sangioni
E. Pompermayer
L.F.P. Gondim
F.S.F. Vogel
author_facet A.M. Antonello
G.C. Cadore
F.L. Pivoto
G. Camillo
P. Braunig
L.A. Sangioni
E. Pompermayer
L.F.P. Gondim
F.S.F. Vogel
author_sort A.M. Antonello
collection DOAJ
description The aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum samples were collected from 195 thoroughbred mares and their newborns in two farms from southern Brazil. Parasite specific antibody responses to Sarcocystis antigens were detected using the indirect immunofluorescent antibody test (IFAT) and immunoblot analysis. In 84.1% (159/189) of the pregnant mares and in 7.4% (14/189) of foals we detected antibodies anti-Sarcocystis spp. by IFAT. All samples seropositive from foals were also positive in their respective mares. Serum samples of seropositive foals by IFAT, showed no reactivity on the immunoblot, having as antigens S. neurona merozoites. In conclusion, the intra-uterine exposure to Sarcocystis spp. antigens in horses was demonstrated, with occurrence not only in mares, but also in their foals, before colostrum ingestion these occurrences were reduced.
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spelling doaj.art-799473fc0536485180220eb9d649ef042022-12-21T18:58:56ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622016-04-0168227127510.1590/1678-4162-8227S0102-09352016000200271Intra-uterine exposure of horses to Sarcocystis spp. antigensA.M. AntonelloG.C. CadoreF.L. PivotoG. CamilloP. BraunigL.A. SangioniE. PompermayerL.F.P. GondimF.S.F. VogelThe aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum samples were collected from 195 thoroughbred mares and their newborns in two farms from southern Brazil. Parasite specific antibody responses to Sarcocystis antigens were detected using the indirect immunofluorescent antibody test (IFAT) and immunoblot analysis. In 84.1% (159/189) of the pregnant mares and in 7.4% (14/189) of foals we detected antibodies anti-Sarcocystis spp. by IFAT. All samples seropositive from foals were also positive in their respective mares. Serum samples of seropositive foals by IFAT, showed no reactivity on the immunoblot, having as antigens S. neurona merozoites. In conclusion, the intra-uterine exposure to Sarcocystis spp. antigens in horses was demonstrated, with occurrence not only in mares, but also in their foals, before colostrum ingestion these occurrences were reduced.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200271&lng=en&tlng=enmieloencefalite protozoária equinainfecção transplacentáriasoroprevalênciareação de imunofluorescência indiretaimmunoblot
spellingShingle A.M. Antonello
G.C. Cadore
F.L. Pivoto
G. Camillo
P. Braunig
L.A. Sangioni
E. Pompermayer
L.F.P. Gondim
F.S.F. Vogel
Intra-uterine exposure of horses to Sarcocystis spp. antigens
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
mieloencefalite protozoária equina
infecção transplacentária
soroprevalência
reação de imunofluorescência indireta
immunoblot
title Intra-uterine exposure of horses to Sarcocystis spp. antigens
title_full Intra-uterine exposure of horses to Sarcocystis spp. antigens
title_fullStr Intra-uterine exposure of horses to Sarcocystis spp. antigens
title_full_unstemmed Intra-uterine exposure of horses to Sarcocystis spp. antigens
title_short Intra-uterine exposure of horses to Sarcocystis spp. antigens
title_sort intra uterine exposure of horses to sarcocystis spp antigens
topic mieloencefalite protozoária equina
infecção transplacentária
soroprevalência
reação de imunofluorescência indireta
immunoblot
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200271&lng=en&tlng=en
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