Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle.
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV chall...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5614567?pdf=render |
| _version_ | 1819108735020171264 |
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| author | Ivana Soria Valeria Quattrocchi Cecilia Langellotti Mariela Gammella Sebastian Digiacomo Beatriz Garcia de la Torre David Andreu Maria Montoya Francisco Sobrino Esther Blanco Patricia Zamorano |
| author_facet | Ivana Soria Valeria Quattrocchi Cecilia Langellotti Mariela Gammella Sebastian Digiacomo Beatriz Garcia de la Torre David Andreu Maria Montoya Francisco Sobrino Esther Blanco Patricia Zamorano |
| author_sort | Ivana Soria |
| collection | DOAJ |
| description | Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential. |
| first_indexed | 2024-12-22T03:14:39Z |
| format | Article |
| id | doaj.art-799498b29351497bb64c99928e4f1158 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:14:39Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-799498b29351497bb64c99928e4f11582022-12-21T18:40:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018518410.1371/journal.pone.0185184Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle.Ivana SoriaValeria QuattrocchiCecilia LangellottiMariela GammellaSebastian DigiacomoBeatriz Garcia de la TorreDavid AndreuMaria MontoyaFrancisco SobrinoEsther BlancoPatricia ZamoranoFoot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136-154)] linked to a T-cell epitope [3A(21-35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21-40] and two or four copies of a B-cell epitope [VP1(135-160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.http://europepmc.org/articles/PMC5614567?pdf=render |
| spellingShingle | Ivana Soria Valeria Quattrocchi Cecilia Langellotti Mariela Gammella Sebastian Digiacomo Beatriz Garcia de la Torre David Andreu Maria Montoya Francisco Sobrino Esther Blanco Patricia Zamorano Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. PLoS ONE |
| title | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. |
| title_full | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. |
| title_fullStr | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. |
| title_full_unstemmed | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. |
| title_short | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle. |
| title_sort | dendrimeric peptides can confer protection against foot and mouth disease virus in cattle |
| url | http://europepmc.org/articles/PMC5614567?pdf=render |
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