Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis
Response surface methodology was employed to optimize the degradation conditions of AFB1 by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volu...
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| Format: | Article |
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MDPI AG
2012-11-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/4/11/1181 |
| _version_ | 1811186225635131392 |
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| author | Jiujiang Yu Shihua Shan Bin Guan Chunjuan Li Cuiping Zhai Qing Kong |
| author_facet | Jiujiang Yu Shihua Shan Bin Guan Chunjuan Li Cuiping Zhai Qing Kong |
| author_sort | Jiujiang Yu |
| collection | DOAJ |
| description | Response surface methodology was employed to optimize the degradation conditions of AFB1 by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volume, inoculum size, agitation speed and incubation time. Central composite design (CCD) and response surface analysis were used to further investigate the interactions between these variables and to optimize the degradation efficiency of R. erythropolis based on a second-order model. The results demonstrated that the optimal parameters were: temperature, 23.2 °C; pH, 7.17; liquid volume, 24.6 mL in 100-mL flask; inoculum size, 10%; agitation speed, 180 rpm; and incubation time, 81.9 h. Under these conditions, the degradation efficiency of R. erythropolis could reach 95.8% in liquid culture, which was increased by about three times as compared to non-optimized conditions. The result by mathematic modeling has great potential for aflatoxin removal in industrial fermentation such as in food processing and ethanol production. |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:42:04Z |
| publishDate | 2012-11-01 |
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| series | Toxins |
| spelling | doaj.art-7996cdb8b5854fe28e75684abbcde2e22022-12-22T04:21:13ZengMDPI AGToxins2072-66512012-11-014111181119510.3390/toxins4111181Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolisJiujiang YuShihua ShanBin GuanChunjuan LiCuiping ZhaiQing KongResponse surface methodology was employed to optimize the degradation conditions of AFB1 by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volume, inoculum size, agitation speed and incubation time. Central composite design (CCD) and response surface analysis were used to further investigate the interactions between these variables and to optimize the degradation efficiency of R. erythropolis based on a second-order model. The results demonstrated that the optimal parameters were: temperature, 23.2 °C; pH, 7.17; liquid volume, 24.6 mL in 100-mL flask; inoculum size, 10%; agitation speed, 180 rpm; and incubation time, 81.9 h. Under these conditions, the degradation efficiency of R. erythropolis could reach 95.8% in liquid culture, which was increased by about three times as compared to non-optimized conditions. The result by mathematic modeling has great potential for aflatoxin removal in industrial fermentation such as in food processing and ethanol production.http://www.mdpi.com/2072-6651/4/11/1181Rhodococcus erythropolisdegradation efficiencyoptimizationPlackett&#8211Burman designcentral composite designresponse surface methodologyaflatoxins |
| spellingShingle | Jiujiang Yu Shihua Shan Bin Guan Chunjuan Li Cuiping Zhai Qing Kong Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis Toxins Rhodococcus erythropolis degradation efficiency optimization Plackett&#8211 Burman design central composite design response surface methodology aflatoxins |
| title | Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis |
| title_full | Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis |
| title_fullStr | Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis |
| title_full_unstemmed | Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis |
| title_short | Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis |
| title_sort | mathematic modeling for optimum conditions on aflatoxin b1 degradation by the aerobic bacterium rhodococcus erythropolis |
| topic | Rhodococcus erythropolis degradation efficiency optimization Plackett&#8211 Burman design central composite design response surface methodology aflatoxins |
| url | http://www.mdpi.com/2072-6651/4/11/1181 |
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