CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections.
Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repea...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3356390?pdf=render |
| _version_ | 1811269141403795456 |
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| author | Laëtitia Fabre Jian Zhang Ghislaine Guigon Simon Le Hello Véronique Guibert Marie Accou-Demartin Saïana de Romans Catherine Lim Chrystelle Roux Virginie Passet Laure Diancourt Martine Guibourdenche Sylvie Issenhuth-Jeanjean Mark Achtman Sylvain Brisse Christophe Sola François-Xavier Weill |
| author_facet | Laëtitia Fabre Jian Zhang Ghislaine Guigon Simon Le Hello Véronique Guibert Marie Accou-Demartin Saïana de Romans Catherine Lim Chrystelle Roux Virginie Passet Laure Diancourt Martine Guibourdenche Sylvie Issenhuth-Jeanjean Mark Achtman Sylvain Brisse Christophe Sola François-Xavier Weill |
| author_sort | Laëtitia Fabre |
| collection | DOAJ |
| description | Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer microevolution discriminated between subtypes within prevalent serotypes, making it possible to carry out typing and subtyping in a single step. We developed a high-throughput subtyping assay for the most prevalent serotype, Typhimurium. An open web-accessible database was set up, providing a serotype/spacer dictionary and an international tool for strain tracking based on this innovative, powerful typing and subtyping tool. |
| first_indexed | 2024-04-12T21:36:44Z |
| format | Article |
| id | doaj.art-7998ecc33d2f4c6cac41979503574110 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:36:44Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7998ecc33d2f4c6cac419795035741102022-12-22T03:15:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3699510.1371/journal.pone.0036995CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections.Laëtitia FabreJian ZhangGhislaine GuigonSimon Le HelloVéronique GuibertMarie Accou-DemartinSaïana de RomansCatherine LimChrystelle RouxVirginie PassetLaure DiancourtMartine GuibourdencheSylvie Issenhuth-JeanjeanMark AchtmanSylvain BrisseChristophe SolaFrançois-Xavier WeillLaboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer microevolution discriminated between subtypes within prevalent serotypes, making it possible to carry out typing and subtyping in a single step. We developed a high-throughput subtyping assay for the most prevalent serotype, Typhimurium. An open web-accessible database was set up, providing a serotype/spacer dictionary and an international tool for strain tracking based on this innovative, powerful typing and subtyping tool.http://europepmc.org/articles/PMC3356390?pdf=render |
| spellingShingle | Laëtitia Fabre Jian Zhang Ghislaine Guigon Simon Le Hello Véronique Guibert Marie Accou-Demartin Saïana de Romans Catherine Lim Chrystelle Roux Virginie Passet Laure Diancourt Martine Guibourdenche Sylvie Issenhuth-Jeanjean Mark Achtman Sylvain Brisse Christophe Sola François-Xavier Weill CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. PLoS ONE |
| title | CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. |
| title_full | CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. |
| title_fullStr | CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. |
| title_full_unstemmed | CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. |
| title_short | CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. |
| title_sort | crispr typing and subtyping for improved laboratory surveillance of salmonella infections |
| url | http://europepmc.org/articles/PMC3356390?pdf=render |
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