Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise
Abstract This study aimed to determine the changes of lipidome in obese women undergoing combined physical exercise training. Fourteen adult women with obesity (mean BMI and age, 33 kg/m2 and 34 ± 5 years), were submitted to combined physical training (aerobic and strength exercises, alternately, 55...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15236-0 |
| _version_ | 1811323695837216768 |
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| author | Rocio San Martin Camila Fernanda Cunha Brandao Márcia Varella Morandi Junqueira-Franco Gizela Pedroso Junqueira Ellen Cristini de Freitas Flavia Giolo de Carvalho Caio Henrique Pinke Rodrigues Audrey Aguesse Stéphanie Billon-Crossouard Michel Krempf Mikaël Croyal Julio Sergio Marchini |
| author_facet | Rocio San Martin Camila Fernanda Cunha Brandao Márcia Varella Morandi Junqueira-Franco Gizela Pedroso Junqueira Ellen Cristini de Freitas Flavia Giolo de Carvalho Caio Henrique Pinke Rodrigues Audrey Aguesse Stéphanie Billon-Crossouard Michel Krempf Mikaël Croyal Julio Sergio Marchini |
| author_sort | Rocio San Martin |
| collection | DOAJ |
| description | Abstract This study aimed to determine the changes of lipidome in obese women undergoing combined physical exercise training. Fourteen adult women with obesity (mean BMI and age, 33 kg/m2 and 34 ± 5 years), were submitted to combined physical training (aerobic and strength exercises, alternately, 55 min at 75–90% of the maximum heart rate, 3 times a week) for 8 weeks. All participants were evaluated before and after the training intervention for lipidome, anthropometric measurements, muscle strength, and maximum oxygen consumption (VO2max). Untargeted liquid chromatography-mass spectrometry analyses allowed the identification of 1252 variables, of which 160 were significant (p < 0.05), and 61 were identified as molecular species of lipids. Volcano plot analysis revealed LPC(16:0p), LPC(18:0p), LPC(20:2), and arachidonic acid upregulated and PC(38:1p), PC(40:4), PC(40:4p) downregulated after combined physical exercise. From the results of the overall Principal component analysis (PCA), the major finding was SM(d18:1/20:0), arachidonic acid, and PC(40:6) species. Other changes included a reduction in waist circumference (Δ = − 2 cm) (p < 0.05), with no weight loss. In conclusion, 8-week of combined exercise training in obese women brought changes in different classes of lipids. This study provides further information to understand the effect of combined physical exercise on lipids related to obesity. |
| first_indexed | 2024-04-13T13:59:53Z |
| format | Article |
| id | doaj.art-79994afc33404f1a9a74c622ae59fb7d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T13:59:53Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-79994afc33404f1a9a74c622ae59fb7d2022-12-22T02:44:04ZengNature PortfolioScientific Reports2045-23222022-07-011211910.1038/s41598-022-15236-0Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exerciseRocio San Martin0Camila Fernanda Cunha Brandao1Márcia Varella Morandi Junqueira-Franco2Gizela Pedroso Junqueira3Ellen Cristini de Freitas4Flavia Giolo de Carvalho5Caio Henrique Pinke Rodrigues6Audrey Aguesse7Stéphanie Billon-Crossouard8Michel Krempf9Mikaël Croyal10Julio Sergio Marchini11Internal Medicine Department, Ribeirão Preto Medical School, University of São Paulo-FMRP USPInternal Medicine Department, Ribeirão Preto Medical School, University of São Paulo-FMRP USPInternal Medicine Department, Ribeirão Preto Medical School, University of São Paulo-FMRP USPDepartment of Health Sciences, Ribeirão Preto Medical School, University of São PauloSchool of Physical Education and Sport of Ribeirão Preto (EEFERP-USP), University of São PauloSchool of Physical Education and Sport of Ribeirão Preto (EEFERP-USP), University of São PauloDepartment of Chemistry, School of Philosophy, Sciences and Letters of Ribeirão Preto, University of São PauloCRNH-Ouest Mass Spectrometry Core FacilityCRNH-Ouest Mass Spectrometry Core FacilityClinique Bretéché, Groupe ElsanCRNH-Ouest Mass Spectrometry Core FacilityInternal Medicine Department, Ribeirão Preto Medical School, University of São Paulo-FMRP USPAbstract This study aimed to determine the changes of lipidome in obese women undergoing combined physical exercise training. Fourteen adult women with obesity (mean BMI and age, 33 kg/m2 and 34 ± 5 years), were submitted to combined physical training (aerobic and strength exercises, alternately, 55 min at 75–90% of the maximum heart rate, 3 times a week) for 8 weeks. All participants were evaluated before and after the training intervention for lipidome, anthropometric measurements, muscle strength, and maximum oxygen consumption (VO2max). Untargeted liquid chromatography-mass spectrometry analyses allowed the identification of 1252 variables, of which 160 were significant (p < 0.05), and 61 were identified as molecular species of lipids. Volcano plot analysis revealed LPC(16:0p), LPC(18:0p), LPC(20:2), and arachidonic acid upregulated and PC(38:1p), PC(40:4), PC(40:4p) downregulated after combined physical exercise. From the results of the overall Principal component analysis (PCA), the major finding was SM(d18:1/20:0), arachidonic acid, and PC(40:6) species. Other changes included a reduction in waist circumference (Δ = − 2 cm) (p < 0.05), with no weight loss. In conclusion, 8-week of combined exercise training in obese women brought changes in different classes of lipids. This study provides further information to understand the effect of combined physical exercise on lipids related to obesity.https://doi.org/10.1038/s41598-022-15236-0 |
| spellingShingle | Rocio San Martin Camila Fernanda Cunha Brandao Márcia Varella Morandi Junqueira-Franco Gizela Pedroso Junqueira Ellen Cristini de Freitas Flavia Giolo de Carvalho Caio Henrique Pinke Rodrigues Audrey Aguesse Stéphanie Billon-Crossouard Michel Krempf Mikaël Croyal Julio Sergio Marchini Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise Scientific Reports |
| title | Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| title_full | Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| title_fullStr | Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| title_full_unstemmed | Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| title_short | Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| title_sort | untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise |
| url | https://doi.org/10.1038/s41598-022-15236-0 |
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