3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds
Gene therapy is one of the most promising approaches in regenerative medicine to restore damaged tissues of various types. However, the ability to control the dose of bioactive molecules in the injection site can be challenging. The combination of genetic constructs, bioresorbable material, and the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/7/421 |
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| author | Maria A. Khvorostina Anton V. Mironov Irina A. Nedorubova Tatiana B. Bukharova Andrey V. Vasilyev Dmitry V. Goldshtein Vladimir S. Komlev Vladimir K. Popov |
| author_facet | Maria A. Khvorostina Anton V. Mironov Irina A. Nedorubova Tatiana B. Bukharova Andrey V. Vasilyev Dmitry V. Goldshtein Vladimir S. Komlev Vladimir K. Popov |
| author_sort | Maria A. Khvorostina |
| collection | DOAJ |
| description | Gene therapy is one of the most promising approaches in regenerative medicine to restore damaged tissues of various types. However, the ability to control the dose of bioactive molecules in the injection site can be challenging. The combination of genetic constructs, bioresorbable material, and the 3D printing technique can help to overcome these difficulties and not only serve as a microenvironment for cell infiltration but also provide localized gene release in a more sustainable way to induce effective cell differentiation. Herein, the cell transfection with plasmid DNA directly incorporated into sodium alginate prior to 3D printing was investigated both in vitro and in vivo. The 3D cryoprinting ensures pDNA structure integrity and safety. 3D printed gene-activated scaffolds (GAS) mediated HEK293 transfection in vitro and effective synthesis of model EGFP protein in vivo, thereby allowing the implementation of the developed GAS in future tissue engineering applications. |
| first_indexed | 2024-03-09T10:18:22Z |
| format | Article |
| id | doaj.art-799b5f1e424540729db2f20791fe8828 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T10:18:22Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-799b5f1e424540729db2f20791fe88282023-12-01T22:10:51ZengMDPI AGGels2310-28612022-07-018742110.3390/gels80704213D Printed Gene-Activated Sodium Alginate Hydrogel ScaffoldsMaria A. Khvorostina0Anton V. Mironov1Irina A. Nedorubova2Tatiana B. Bukharova3Andrey V. Vasilyev4Dmitry V. Goldshtein5Vladimir S. Komlev6Vladimir K. Popov7Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, RussiaInstitute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, RussiaResearch Centre for Medical Genetics, Moscow 115478, RussiaResearch Centre for Medical Genetics, Moscow 115478, RussiaResearch Centre for Medical Genetics, Moscow 115478, RussiaResearch Centre for Medical Genetics, Moscow 115478, RussiaA.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences, Moscow 119334, RussiaInstitute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 108840, RussiaGene therapy is one of the most promising approaches in regenerative medicine to restore damaged tissues of various types. However, the ability to control the dose of bioactive molecules in the injection site can be challenging. The combination of genetic constructs, bioresorbable material, and the 3D printing technique can help to overcome these difficulties and not only serve as a microenvironment for cell infiltration but also provide localized gene release in a more sustainable way to induce effective cell differentiation. Herein, the cell transfection with plasmid DNA directly incorporated into sodium alginate prior to 3D printing was investigated both in vitro and in vivo. The 3D cryoprinting ensures pDNA structure integrity and safety. 3D printed gene-activated scaffolds (GAS) mediated HEK293 transfection in vitro and effective synthesis of model EGFP protein in vivo, thereby allowing the implementation of the developed GAS in future tissue engineering applications.https://www.mdpi.com/2310-2861/8/7/421gene-activated scaffolds3D printingsodium alginate hydrogelplasmid DNA |
| spellingShingle | Maria A. Khvorostina Anton V. Mironov Irina A. Nedorubova Tatiana B. Bukharova Andrey V. Vasilyev Dmitry V. Goldshtein Vladimir S. Komlev Vladimir K. Popov 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds Gels gene-activated scaffolds 3D printing sodium alginate hydrogel plasmid DNA |
| title | 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds |
| title_full | 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds |
| title_fullStr | 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds |
| title_full_unstemmed | 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds |
| title_short | 3D Printed Gene-Activated Sodium Alginate Hydrogel Scaffolds |
| title_sort | 3d printed gene activated sodium alginate hydrogel scaffolds |
| topic | gene-activated scaffolds 3D printing sodium alginate hydrogel plasmid DNA |
| url | https://www.mdpi.com/2310-2861/8/7/421 |
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