The development of proximity labeling technology and its applications in mammals, plants, and microorganisms
Abstract Protein‒protein, protein‒RNA, and protein‒DNA interaction networks form the basis of cellular regulation and signal transduction, making it crucial to explore these interaction networks to understand complex biological processes. Traditional methods such as affinity purification and yeast t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Cell Communication and Signaling |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12964-023-01310-1 |
| _version_ | 1797557631008636928 |
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| author | Jieyu Guo Shuang Guo Siao Lu Jun Gong Long Wang Liqiong Ding Qingjie Chen Wu Liu |
| author_facet | Jieyu Guo Shuang Guo Siao Lu Jun Gong Long Wang Liqiong Ding Qingjie Chen Wu Liu |
| author_sort | Jieyu Guo |
| collection | DOAJ |
| description | Abstract Protein‒protein, protein‒RNA, and protein‒DNA interaction networks form the basis of cellular regulation and signal transduction, making it crucial to explore these interaction networks to understand complex biological processes. Traditional methods such as affinity purification and yeast two-hybrid assays have been shown to have limitations, as they can only isolate high-affinity molecular interactions under nonphysiological conditions or in vitro. Moreover, these methods have shortcomings for organelle isolation and protein subcellular localization. To address these issues, proximity labeling techniques have been developed. This technology not only overcomes the limitations of traditional methods but also offers unique advantages in studying protein spatial characteristics and molecular interactions within living cells. Currently, this technique not only is indispensable in research on mammalian nucleoprotein interactions but also provides a reliable approach for studying nonmammalian cells, such as plants, parasites and viruses. Given these advantages, this article provides a detailed introduction to the principles of proximity labeling techniques and the development of labeling enzymes. The focus is on summarizing the recent applications of TurboID and miniTurbo in mammals, plants, and microorganisms. Video Abstract |
| first_indexed | 2024-03-10T17:20:21Z |
| format | Article |
| id | doaj.art-799bf1f5074c4df399069d6cf42c7240 |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-03-10T17:20:21Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-799bf1f5074c4df399069d6cf42c72402023-11-20T10:22:16ZengBMCCell Communication and Signaling1478-811X2023-09-0121112210.1186/s12964-023-01310-1The development of proximity labeling technology and its applications in mammals, plants, and microorganismsJieyu Guo0Shuang Guo1Siao Lu2Jun Gong3Long Wang4Liqiong Ding5Qingjie Chen6Wu Liu7School of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and TechnologyMedicine Research Institute, Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and TechnologySchool of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and TechnologySchool of Pharmacy, Xianning Medical College, Hubei University of Science and TechnologySchool of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and TechnologySchool of Pharmacy, Xianning Medical College, Hubei University of Science and TechnologySchool of Pharmacy, Xianning Medical College, Hubei University of Science and TechnologySchool of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and TechnologyAbstract Protein‒protein, protein‒RNA, and protein‒DNA interaction networks form the basis of cellular regulation and signal transduction, making it crucial to explore these interaction networks to understand complex biological processes. Traditional methods such as affinity purification and yeast two-hybrid assays have been shown to have limitations, as they can only isolate high-affinity molecular interactions under nonphysiological conditions or in vitro. Moreover, these methods have shortcomings for organelle isolation and protein subcellular localization. To address these issues, proximity labeling techniques have been developed. This technology not only overcomes the limitations of traditional methods but also offers unique advantages in studying protein spatial characteristics and molecular interactions within living cells. Currently, this technique not only is indispensable in research on mammalian nucleoprotein interactions but also provides a reliable approach for studying nonmammalian cells, such as plants, parasites and viruses. Given these advantages, this article provides a detailed introduction to the principles of proximity labeling techniques and the development of labeling enzymes. The focus is on summarizing the recent applications of TurboID and miniTurbo in mammals, plants, and microorganisms. Video Abstracthttps://doi.org/10.1186/s12964-023-01310-1Proximity labelingAPEXBioIDTurboIDminiTurbo |
| spellingShingle | Jieyu Guo Shuang Guo Siao Lu Jun Gong Long Wang Liqiong Ding Qingjie Chen Wu Liu The development of proximity labeling technology and its applications in mammals, plants, and microorganisms Cell Communication and Signaling Proximity labeling APEX BioID TurboID miniTurbo |
| title | The development of proximity labeling technology and its applications in mammals, plants, and microorganisms |
| title_full | The development of proximity labeling technology and its applications in mammals, plants, and microorganisms |
| title_fullStr | The development of proximity labeling technology and its applications in mammals, plants, and microorganisms |
| title_full_unstemmed | The development of proximity labeling technology and its applications in mammals, plants, and microorganisms |
| title_short | The development of proximity labeling technology and its applications in mammals, plants, and microorganisms |
| title_sort | development of proximity labeling technology and its applications in mammals plants and microorganisms |
| topic | Proximity labeling APEX BioID TurboID miniTurbo |
| url | https://doi.org/10.1186/s12964-023-01310-1 |
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