Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model
Background and study aims: Epidermal cell sheet (ECS) transplantation immediately after aggressive endoscopic submucosal dissection (ESD) has been shown to be safe and effective in the prevention of esophageal strictures. This study evaluated the feasibility of ECS transplantation after endoscopic b...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Georg Thieme Verlag KG
2016-10-01
|
| Series: | Endoscopy International Open |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-116145 |
| _version_ | 1828899679887163392 |
|---|---|
| author | Shinichiro Kobayashi Nobuo Kanai Nobuyuki Tanaka Masanori Maeda Takahiro Hosoi Fumio Fukai Susumu Eguchi Masayuki Yamato |
| author_facet | Shinichiro Kobayashi Nobuo Kanai Nobuyuki Tanaka Masanori Maeda Takahiro Hosoi Fumio Fukai Susumu Eguchi Masayuki Yamato |
| author_sort | Shinichiro Kobayashi |
| collection | DOAJ |
| description | Background and study aims: Epidermal cell sheet (ECS) transplantation immediately after aggressive endoscopic submucosal dissection (ESD) has been shown to be safe and effective in the prevention of esophageal strictures. This study evaluated the feasibility of ECS transplantation after endoscopic balloon dilation (EBD) in a porcine model.
Methods: Six pigs underwent circumferential esophageal ESD under general anesthesia. Two weeks later, two pigs underwent EBD and transplantation of an autologous ECS, two underwent EBD alone, and two underwent endoscopic observation only (control).
Results: The two pigs in the transplantation group underwent six ECS transplants after EBD with five of the six (83 %) being successful, as shown by engraftment of transplanted ECSs after 7 days. No adverse events were observed. Stricture rates were lower in the two transplanted pigs (55 % and 60 %) than in the control (92.2 % and 87.7 %) and EBD-treated (71.7 % and 78.2 %) pigs. Infiltration of inflammatory cells was significantly lower in the transplanted pigs than in the control and EBD-treated pigs.
Conclusion: Preliminary results indicate the stability of the ECS transplantation procedure and the engraftment of transplanted ECS in the tears after EBD. This proof-of-concept study suggests that covering tears with ECSs after EBD may avoid re-strictures. |
| first_indexed | 2024-12-13T15:34:57Z |
| format | Article |
| id | doaj.art-799ce07214b04194b5bc7318db291a60 |
| institution | Directory Open Access Journal |
| issn | 2364-3722 2196-9736 |
| language | English |
| last_indexed | 2024-12-13T15:34:57Z |
| publishDate | 2016-10-01 |
| publisher | Georg Thieme Verlag KG |
| record_format | Article |
| series | Endoscopy International Open |
| spelling | doaj.art-799ce07214b04194b5bc7318db291a602022-12-21T23:40:03ZengGeorg Thieme Verlag KGEndoscopy International Open2364-37222196-97362016-10-010411E1116E112310.1055/s-0042-116145Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine modelShinichiro Kobayashi0Nobuo Kanai1Nobuyuki Tanaka2Masanori Maeda3Takahiro Hosoi4Fumio Fukai5Susumu Eguchi6Masayuki Yamato7Department of Surgery, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Pharmaceutical Sciences, Graduate School of Pharmaceutical Science, Tokyo University of Science, Noda, JapanDepartment of Surgery, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, JapanBackground and study aims: Epidermal cell sheet (ECS) transplantation immediately after aggressive endoscopic submucosal dissection (ESD) has been shown to be safe and effective in the prevention of esophageal strictures. This study evaluated the feasibility of ECS transplantation after endoscopic balloon dilation (EBD) in a porcine model. Methods: Six pigs underwent circumferential esophageal ESD under general anesthesia. Two weeks later, two pigs underwent EBD and transplantation of an autologous ECS, two underwent EBD alone, and two underwent endoscopic observation only (control). Results: The two pigs in the transplantation group underwent six ECS transplants after EBD with five of the six (83 %) being successful, as shown by engraftment of transplanted ECSs after 7 days. No adverse events were observed. Stricture rates were lower in the two transplanted pigs (55 % and 60 %) than in the control (92.2 % and 87.7 %) and EBD-treated (71.7 % and 78.2 %) pigs. Infiltration of inflammatory cells was significantly lower in the transplanted pigs than in the control and EBD-treated pigs. Conclusion: Preliminary results indicate the stability of the ECS transplantation procedure and the engraftment of transplanted ECS in the tears after EBD. This proof-of-concept study suggests that covering tears with ECSs after EBD may avoid re-strictures.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-116145 |
| spellingShingle | Shinichiro Kobayashi Nobuo Kanai Nobuyuki Tanaka Masanori Maeda Takahiro Hosoi Fumio Fukai Susumu Eguchi Masayuki Yamato Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model Endoscopy International Open |
| title | Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model |
| title_full | Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model |
| title_fullStr | Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model |
| title_full_unstemmed | Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model |
| title_short | Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model |
| title_sort | transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re strictures initial experience in a porcine model |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-116145 |
| work_keys_str_mv | AT shinichirokobayashi transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT nobuokanai transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT nobuyukitanaka transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT masanorimaeda transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT takahirohosoi transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT fumiofukai transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT susumueguchi transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel AT masayukiyamato transplantationofepidermalcellsheetsbyendoscopicballoondilatationtoavoidesophagealrestricturesinitialexperienceinaporcinemodel |