Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro
Amyotrophic lateral sclerosis (ALS) is a common degenerative disease of the central nervous system concerning a progressive loss of upper and lower motor neurons. While 5%–10% of patients are diagnosed with the inherited form of the disease, the vast majority of patients suffer from the less charact...
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MDPI AG
2020-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/6/460 |
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| author | Mareike Zwilling Carsten Theiss Veronika Matschke |
| author_facet | Mareike Zwilling Carsten Theiss Veronika Matschke |
| author_sort | Mareike Zwilling |
| collection | DOAJ |
| description | Amyotrophic lateral sclerosis (ALS) is a common degenerative disease of the central nervous system concerning a progressive loss of upper and lower motor neurons. While 5%–10% of patients are diagnosed with the inherited form of the disease, the vast majority of patients suffer from the less characterized sporadic form of ALS (sALS). As the wobbler mouse and the ALS show striking similarities in view of phenotypical attributes, the mouse is rated as an animal model for the disease. Recent investigations show the importance of nicotinamide adenine dinucleotide (NAD<sup>+</sup>) and its producing enzyme nicotinic acid mononucleotide transferase 2 (Nmnat2) for neurodegeneration as well as for the preservation of health of the neuronal cells. Furthermore, it is newly determined that these molecules show significant downregulations in the spinal cord of wobbler mice in the stable phase of disease development. Here, we were able to prove a positive benefit on affected motor neurons from an additional NAD<sup>+</sup> supply as well as an increase in the Nmnat2 level through caffeine treatment in cells in vitro. In addition, first assumptions about the importance of endogenous and exogenous factors that have an influence on the wellbeing of motor nerve cells in the model of ALS can be considered. |
| first_indexed | 2024-03-10T19:32:44Z |
| format | Article |
| id | doaj.art-799e82e72cc84bbbbd47ccd0941ccc74 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T19:32:44Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-799e82e72cc84bbbbd47ccd0941ccc742023-11-20T01:57:56ZengMDPI AGAntioxidants2076-39212020-05-019646010.3390/antiox9060460Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In VitroMareike Zwilling0Carsten Theiss1Veronika Matschke2Department of Cytology, Institute of Anatomy, Medical Faculty, Ruhr University Bochum, D-44801 Bochum, GermanyDepartment of Cytology, Institute of Anatomy, Medical Faculty, Ruhr University Bochum, D-44801 Bochum, GermanyDepartment of Cytology, Institute of Anatomy, Medical Faculty, Ruhr University Bochum, D-44801 Bochum, GermanyAmyotrophic lateral sclerosis (ALS) is a common degenerative disease of the central nervous system concerning a progressive loss of upper and lower motor neurons. While 5%–10% of patients are diagnosed with the inherited form of the disease, the vast majority of patients suffer from the less characterized sporadic form of ALS (sALS). As the wobbler mouse and the ALS show striking similarities in view of phenotypical attributes, the mouse is rated as an animal model for the disease. Recent investigations show the importance of nicotinamide adenine dinucleotide (NAD<sup>+</sup>) and its producing enzyme nicotinic acid mononucleotide transferase 2 (Nmnat2) for neurodegeneration as well as for the preservation of health of the neuronal cells. Furthermore, it is newly determined that these molecules show significant downregulations in the spinal cord of wobbler mice in the stable phase of disease development. Here, we were able to prove a positive benefit on affected motor neurons from an additional NAD<sup>+</sup> supply as well as an increase in the Nmnat2 level through caffeine treatment in cells in vitro. In addition, first assumptions about the importance of endogenous and exogenous factors that have an influence on the wellbeing of motor nerve cells in the model of ALS can be considered.https://www.mdpi.com/2076-3921/9/6/460amyotrophic lateral sclerosisALSmotor neuron diseaseneuroprotectionROSdegeneration |
| spellingShingle | Mareike Zwilling Carsten Theiss Veronika Matschke Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro Antioxidants amyotrophic lateral sclerosis ALS motor neuron disease neuroprotection ROS degeneration |
| title | Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro |
| title_full | Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro |
| title_fullStr | Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro |
| title_full_unstemmed | Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro |
| title_short | Caffeine and NAD<sup>+</sup> Improve Motor Neural Integrity of Dissociated Wobbler Cells In Vitro |
| title_sort | caffeine and nad sup sup improve motor neural integrity of dissociated wobbler cells in vitro |
| topic | amyotrophic lateral sclerosis ALS motor neuron disease neuroprotection ROS degeneration |
| url | https://www.mdpi.com/2076-3921/9/6/460 |
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