Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG
Francisella tularensis is an intracellular bacterium that causes tularemia. Progress in creating a safe and effective vaccine for the prevention of tularemia is challenging due to a lack of knowledge about immunological parameters indicative of protective adaptive immunity. Objective of the research...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2020-10-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1365 |
| _version_ | 1797220807262339072 |
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| author | A. S. Kartseva O. V. Kalmantaeva M. V. Silkina T. I. Kombarova V. M. Pavlov A. N. Mokrievich V. V. Firstova |
| author_facet | A. S. Kartseva O. V. Kalmantaeva M. V. Silkina T. I. Kombarova V. M. Pavlov A. N. Mokrievich V. V. Firstova |
| author_sort | A. S. Kartseva |
| collection | DOAJ |
| description | Francisella tularensis is an intracellular bacterium that causes tularemia. Progress in creating a safe and effective vaccine for the prevention of tularemia is challenging due to a lack of knowledge about immunological parameters indicative of protective adaptive immunity. Objective of the research was to assess the effect of modifications of the F. tularensis 15 NIIEG genome on the immunogenic and protective properties of F. tularensis 15/23-1ΔrecA and F. tularensis 15/23-1/sodBΔrecA strains. Materials and methods. Multi-parameter flow cytometry and the measurement of secreted cytokines were used to characterize the responses of mouse spleen lymphocytes in response to re-stimulation of F. tularensis with acid-insoluble complex (AIC) in vitro. Also, the titers of specific antibodies to F. tularensis lipopolysaccharide in blood serum were analyzed by enzyme-linked immunosorbent assay. Results and discussion. It has been shown that immunization with the studied strains led to a significant increase in CD4+ and/or CD8+ T cells capable of expressing functional markers: CD69, CD25 and/or CD28; an increase in the subpopulation of T-helpers synthesizing IFN-γ. In the body of immune mice, a pool of B-lymphocytes was formed, capable of secreting IFN-γ in response to their stimulation with AIC. Immunization with the strain 15/23-1/sodBΔrecA provided 70% protection in mice from intranasal infection with a virulent strain of F. tularensis SchuS4. More pronounced protective properties were associated with the activation of not only B-lymphocytes and T-helpers, but also with the simultaneous activation of cytotoxic T-lymphocytes. |
| first_indexed | 2024-03-08T13:41:23Z |
| format | Article |
| id | doaj.art-79a4472219244325836fcac8859e2970 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:55:24Z |
| publishDate | 2020-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-79a4472219244325836fcac8859e29702024-04-05T16:47:15ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2020-10-0103626910.21055/0370-1069-2020-3-62-691223Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEGA. S. Kartseva0O. V. Kalmantaeva1M. V. Silkina2T. I. Kombarova3V. M. Pavlov4A. N. Mokrievich5V. V. Firstova6State Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyState Scientific Center for Applied Microbiology and BiotechnologyFrancisella tularensis is an intracellular bacterium that causes tularemia. Progress in creating a safe and effective vaccine for the prevention of tularemia is challenging due to a lack of knowledge about immunological parameters indicative of protective adaptive immunity. Objective of the research was to assess the effect of modifications of the F. tularensis 15 NIIEG genome on the immunogenic and protective properties of F. tularensis 15/23-1ΔrecA and F. tularensis 15/23-1/sodBΔrecA strains. Materials and methods. Multi-parameter flow cytometry and the measurement of secreted cytokines were used to characterize the responses of mouse spleen lymphocytes in response to re-stimulation of F. tularensis with acid-insoluble complex (AIC) in vitro. Also, the titers of specific antibodies to F. tularensis lipopolysaccharide in blood serum were analyzed by enzyme-linked immunosorbent assay. Results and discussion. It has been shown that immunization with the studied strains led to a significant increase in CD4+ and/or CD8+ T cells capable of expressing functional markers: CD69, CD25 and/or CD28; an increase in the subpopulation of T-helpers synthesizing IFN-γ. In the body of immune mice, a pool of B-lymphocytes was formed, capable of secreting IFN-γ in response to their stimulation with AIC. Immunization with the strain 15/23-1/sodBΔrecA provided 70% protection in mice from intranasal infection with a virulent strain of F. tularensis SchuS4. More pronounced protective properties were associated with the activation of not only B-lymphocytes and T-helpers, but also with the simultaneous activation of cytotoxic T-lymphocytes.https://journal.microbe.ru/jour/article/view/1365tularemiavaccinet-lymphocytescytokinesb-lymphocytesprotectivity |
| spellingShingle | A. S. Kartseva O. V. Kalmantaeva M. V. Silkina T. I. Kombarova V. M. Pavlov A. N. Mokrievich V. V. Firstova Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG Проблемы особо опасных инфекций tularemia vaccine t-lymphocytes cytokines b-lymphocytes protectivity |
| title | Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG |
| title_full | Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG |
| title_fullStr | Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG |
| title_full_unstemmed | Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG |
| title_short | Characterization of Immunogenic and Protective Properties of the Modified Variants of the Strain Francisella tularensis 15 NIIEG |
| title_sort | characterization of immunogenic and protective properties of the modified variants of the strain francisella tularensis 15 niieg |
| topic | tularemia vaccine t-lymphocytes cytokines b-lymphocytes protectivity |
| url | https://journal.microbe.ru/jour/article/view/1365 |
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