Identification of endogenous reference genes for the analysis of microRNA expression in the hippocampus of the pilocarpine-induced model of mesial temporal lobe epilepsy.

Real-time quantitative RT-PCR (qPCR) is one of the most powerful techniques for analyzing miRNA expression because of its sensitivity and specificity. However, in this type of analysis, a suitable normalizer is required to ensure that gene expression is unaffected by the experimental condition. To t...

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Bibliographic Details
Main Authors: Mykaella Andrade de Araújo, Thalita Ewellyn Batista Sales Marques, Jamile Taniele-Silva, Fernanda Maria de Araújo Souza, Tiago Gomes de Andrade, Norberto Garcia-Cairasco, Maria Luisa Paçó-Larson, Daniel Leite Góes Gitaí
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4070922?pdf=render