Predictive role BLVRA mRNA expression in hepatocellular cancer
Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-11-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119311147 |
| _version_ | 1818899889332944896 |
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| author | Kristýna Kubícková Iva Subhanová Renáta Konícková Linda Matousová Petr Urbánek Hana Parobková Martin Kupec Jirí Pudil Libor Vítek |
| author_facet | Kristýna Kubícková Iva Subhanová Renáta Konícková Linda Matousová Petr Urbánek Hana Parobková Martin Kupec Jirí Pudil Libor Vítek |
| author_sort | Kristýna Kubícková |
| collection | DOAJ |
| description | Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of the genes involved in the homeostasis of oxidative stress in patients with HCC.Material and methods. The study was performed on 32 patients with primary HCC (verified by liver histology in 29 patients) and 27 control subjects (in 11 subjects, liver histology was available either with no or minimal changes in the liver tissue). Gene expressions of heme oxygenase 1 (HMOX1), biliverdin reductase A/B (BLVRA/B), NADPH oxidase 2 (NOX2)and p22phox were analyzed in the liver and peripheral blood leukocytes (PBL) in the subjects.Results. Compared to controls, almost a 3 times higher mRNA level of BLVRA was detected in livers of HCC patients (p = 0.002); while those of BLVRB as well as HMOX1 were unchanged (p > 0.05). In accord with these results in the liver tissue, BLVRA mRNA levels in PBL were also significantly increased in HCC patients (p = 0.012). mRNA levels of NOX2 and p22phox in the liver tissue, although higher in HCC patients, did not differ significantly compared to control subjects (p > 0.05). Nevertheless, NOX2 mRNA level in PBL was significantly higher in HCC patients (p = 0.003).Conclusions. BLVRA mRNA levels in the liver as well as in PBL are significantly higher in HCC patients most likely as a feedback mechanism to control increased oxidative stress associated with HCC progression. |
| first_indexed | 2024-12-19T19:55:08Z |
| format | Article |
| id | doaj.art-79a5426091274368813d79c78c405d18 |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-19T19:55:08Z |
| publishDate | 2016-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-79a5426091274368813d79c78c405d182022-12-21T20:07:50ZengElsevierAnnals of Hepatology1665-26812016-11-01156881887Predictive role BLVRA mRNA expression in hepatocellular cancerKristýna Kubícková0Iva Subhanová1Renáta Konícková2Linda Matousová3Petr Urbánek4Hana Parobková5Martin Kupec6Jirí Pudil7Libor Vítek8Department of Internal Medicine, 1st Faculty of Medicine, Charles University in Prague, and Military University Hospital, Prague, Czech RepublicInstitute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, and 1st Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicInstitute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, and 1st Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicInstitute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, and 1st Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Internal Medicine, 1st Faculty of Medicine, Charles University in Prague, and Military University Hospital, Prague, Czech RepublicDepartment of Radiology, Military University Hospital, Prague, Czech RepublicDepartment of Oncology, 1st Faculty of Medicine, Charles University in Prague, and Thomayer Hospital, Prague, Czech Republic, Prague, Czech RepublicDepartment of Surgery, 2nd Faculty of Medicine, Charles University in Prague, and Military University Hospital, Prague, Czech RepublicInstitute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, and 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; 4th Department of Internal Medicine, General University Hospital, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; Correspondence and reprint request:Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of the genes involved in the homeostasis of oxidative stress in patients with HCC.Material and methods. The study was performed on 32 patients with primary HCC (verified by liver histology in 29 patients) and 27 control subjects (in 11 subjects, liver histology was available either with no or minimal changes in the liver tissue). Gene expressions of heme oxygenase 1 (HMOX1), biliverdin reductase A/B (BLVRA/B), NADPH oxidase 2 (NOX2)and p22phox were analyzed in the liver and peripheral blood leukocytes (PBL) in the subjects.Results. Compared to controls, almost a 3 times higher mRNA level of BLVRA was detected in livers of HCC patients (p = 0.002); while those of BLVRB as well as HMOX1 were unchanged (p > 0.05). In accord with these results in the liver tissue, BLVRA mRNA levels in PBL were also significantly increased in HCC patients (p = 0.012). mRNA levels of NOX2 and p22phox in the liver tissue, although higher in HCC patients, did not differ significantly compared to control subjects (p > 0.05). Nevertheless, NOX2 mRNA level in PBL was significantly higher in HCC patients (p = 0.003).Conclusions. BLVRA mRNA levels in the liver as well as in PBL are significantly higher in HCC patients most likely as a feedback mechanism to control increased oxidative stress associated with HCC progression.http://www.sciencedirect.com/science/article/pii/S1665268119311147Biliverdin reductaseHeme catabolic pathwayHeme oxygenaseLiver cirrhosisOxidative stress |
| spellingShingle | Kristýna Kubícková Iva Subhanová Renáta Konícková Linda Matousová Petr Urbánek Hana Parobková Martin Kupec Jirí Pudil Libor Vítek Predictive role BLVRA mRNA expression in hepatocellular cancer Annals of Hepatology Biliverdin reductase Heme catabolic pathway Heme oxygenase Liver cirrhosis Oxidative stress |
| title | Predictive role BLVRA mRNA expression in hepatocellular cancer |
| title_full | Predictive role BLVRA mRNA expression in hepatocellular cancer |
| title_fullStr | Predictive role BLVRA mRNA expression in hepatocellular cancer |
| title_full_unstemmed | Predictive role BLVRA mRNA expression in hepatocellular cancer |
| title_short | Predictive role BLVRA mRNA expression in hepatocellular cancer |
| title_sort | predictive role blvra mrna expression in hepatocellular cancer |
| topic | Biliverdin reductase Heme catabolic pathway Heme oxygenase Liver cirrhosis Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119311147 |
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