Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light
Abstract Background Marine diatoms have a higher fucoxanthin content in comparison to macroalgae. Fucoxanthin features many potent bioactive properties, particularly anti-obesity properties. Despite the great potential for harvesting larger amounts of fucoxanthin, the impacts of light quality (light...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-018-0957-0 |
| _version_ | 1818996354001666048 |
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| author | Song Wang Sujit K. Verma Inamullah Hakeem Said Laurenz Thomsen Matthias S. Ullrich Nikolai Kuhnert |
| author_facet | Song Wang Sujit K. Verma Inamullah Hakeem Said Laurenz Thomsen Matthias S. Ullrich Nikolai Kuhnert |
| author_sort | Song Wang |
| collection | DOAJ |
| description | Abstract Background Marine diatoms have a higher fucoxanthin content in comparison to macroalgae. Fucoxanthin features many potent bioactive properties, particularly anti-obesity properties. Despite the great potential for harvesting larger amounts of fucoxanthin, the impacts of light quality (light source, intensity, and photoperiod) on fucoxanthin production and the essential proteins involved in fucoxanthin biosynthesis in marine diatoms remain unclear. Results In the present study, Cylindrotheca closterium was selected from four different species of diatoms based on its high fucoxanthin content and productivity. Optimal light conditions (light source, intensity, and regime) were determined by a “Design of Experiment” approach (software MODDE Pro 11 was used). The model indicated that an 18/6 light/darkness regime increased fucoxanthin productivity remarkably as opposed to a 12/12 or 24/0 regime. Eventually, blue light-emitting diode light, as an alternative to fluorescent light, at 100 μmol/m2/s and 18/6 light/darkness regime yielded maximum fucoxanthin productivity and minimal energy consumption. The fucoxanthin production of C. closterium under the predicted optimal light conditions was assessed both in bottle and bag photobioreactors (PBRs). The high fucoxanthin content (25.5 mg/g) obtained from bag PBRs demonstrated the feasibility of large-scale production. The proteomes of C. closterium under the most favorable and unfavorable fucoxanthin biosynthesis light/darkness regimes (18/6 and 24/0, respectively) were compared to identify the essential proteins associated with fucoxanthin accumulation by matrix-assisted laser desorption/ionization-time of flight–mass spectrometry. Six proteins that were up-regulated in the 18/6 regime but down-regulated in the 24/0 were identified as important chloroplastic proteins involved in photosynthesis, energy metabolism, and cellular processes. Conclusions Blue light-emitting diode light at 100 μmol/m2/s and 18/6 light/darkness regime induced maximum fucoxanthin productivity in C. closterium and minimized energy consumption. The high fucoxanthin production in the bag photobioreactor under optimal light conditions demonstrated the possibility of commercialization. Proteomics suggests that fucoxanthin biosynthesis is intimately associated with the photosynthetic efficiency of the diatom, providing another technical and bioengineering outlook on fucoxanthin enhancement. |
| first_indexed | 2024-12-20T21:28:24Z |
| format | Article |
| id | doaj.art-79a8b81e651e4981948586e1117b8340 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-20T21:28:24Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-79a8b81e651e4981948586e1117b83402022-12-21T19:26:05ZengBMCMicrobial Cell Factories1475-28592018-07-0117111310.1186/s12934-018-0957-0Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode lightSong Wang0Sujit K. Verma1Inamullah Hakeem Said2Laurenz Thomsen3Matthias S. Ullrich4Nikolai Kuhnert5Department of Physics and Earth Sciences, Jacobs University BremenDepartment of Life Science and Chemistry, Jacobs University BremenDepartment of Life Science and Chemistry, Jacobs University BremenDepartment of Physics and Earth Sciences, Jacobs University BremenDepartment of Life Science and Chemistry, Jacobs University BremenDepartment of Life Science and Chemistry, Jacobs University BremenAbstract Background Marine diatoms have a higher fucoxanthin content in comparison to macroalgae. Fucoxanthin features many potent bioactive properties, particularly anti-obesity properties. Despite the great potential for harvesting larger amounts of fucoxanthin, the impacts of light quality (light source, intensity, and photoperiod) on fucoxanthin production and the essential proteins involved in fucoxanthin biosynthesis in marine diatoms remain unclear. Results In the present study, Cylindrotheca closterium was selected from four different species of diatoms based on its high fucoxanthin content and productivity. Optimal light conditions (light source, intensity, and regime) were determined by a “Design of Experiment” approach (software MODDE Pro 11 was used). The model indicated that an 18/6 light/darkness regime increased fucoxanthin productivity remarkably as opposed to a 12/12 or 24/0 regime. Eventually, blue light-emitting diode light, as an alternative to fluorescent light, at 100 μmol/m2/s and 18/6 light/darkness regime yielded maximum fucoxanthin productivity and minimal energy consumption. The fucoxanthin production of C. closterium under the predicted optimal light conditions was assessed both in bottle and bag photobioreactors (PBRs). The high fucoxanthin content (25.5 mg/g) obtained from bag PBRs demonstrated the feasibility of large-scale production. The proteomes of C. closterium under the most favorable and unfavorable fucoxanthin biosynthesis light/darkness regimes (18/6 and 24/0, respectively) were compared to identify the essential proteins associated with fucoxanthin accumulation by matrix-assisted laser desorption/ionization-time of flight–mass spectrometry. Six proteins that were up-regulated in the 18/6 regime but down-regulated in the 24/0 were identified as important chloroplastic proteins involved in photosynthesis, energy metabolism, and cellular processes. Conclusions Blue light-emitting diode light at 100 μmol/m2/s and 18/6 light/darkness regime induced maximum fucoxanthin productivity in C. closterium and minimized energy consumption. The high fucoxanthin production in the bag photobioreactor under optimal light conditions demonstrated the possibility of commercialization. Proteomics suggests that fucoxanthin biosynthesis is intimately associated with the photosynthetic efficiency of the diatom, providing another technical and bioengineering outlook on fucoxanthin enhancement.http://link.springer.com/article/10.1186/s12934-018-0957-0DiatomFucoxanthinPhotobioreactorProteomicsPhotosynthesis |
| spellingShingle | Song Wang Sujit K. Verma Inamullah Hakeem Said Laurenz Thomsen Matthias S. Ullrich Nikolai Kuhnert Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light Microbial Cell Factories Diatom Fucoxanthin Photobioreactor Proteomics Photosynthesis |
| title | Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light |
| title_full | Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light |
| title_fullStr | Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light |
| title_full_unstemmed | Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light |
| title_short | Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light |
| title_sort | changes in the fucoxanthin production and protein profiles in cylindrotheca closterium in response to blue light emitting diode light |
| topic | Diatom Fucoxanthin Photobioreactor Proteomics Photosynthesis |
| url | http://link.springer.com/article/10.1186/s12934-018-0957-0 |
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