Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa
Human herpesvirus 8 (HHV-8) is the etiological agent of all forms of Kaposi’s sarcoma (KS). K1 gene studies have identified five major molecular genotypes with geographical clustering. This study described the epidemiology of HHV-8 and its molecular diversity in Gabon among Bantu and Pygmy adult rur...
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| Online Access: | https://www.mdpi.com/1999-4915/13/2/175 |
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| author | Antony Idam Mamimandjiami Augustin Mouinga-Ondémé Jill-Léa Ramassamy Délia Doreen Djuicy Philippe V. Afonso Antoine Mahé Jean-Bernard Lekana-Douki Olivier Cassar Antoine Gessain |
| author_facet | Antony Idam Mamimandjiami Augustin Mouinga-Ondémé Jill-Léa Ramassamy Délia Doreen Djuicy Philippe V. Afonso Antoine Mahé Jean-Bernard Lekana-Douki Olivier Cassar Antoine Gessain |
| author_sort | Antony Idam Mamimandjiami |
| collection | DOAJ |
| description | Human herpesvirus 8 (HHV-8) is the etiological agent of all forms of Kaposi’s sarcoma (KS). K1 gene studies have identified five major molecular genotypes with geographical clustering. This study described the epidemiology of HHV-8 and its molecular diversity in Gabon among Bantu and Pygmy adult rural populations and KS patients. Plasma antibodies against latency-associated nuclear antigens (LANA) were searched by indirect immunofluorescence. Buffy coat DNA samples were subjected to polymerase chain reaction (PCR) to obtain a K1 gene fragment. We studied 1020 persons; 91% were Bantus and 9% Pygmies. HHV-8 seroprevalence was 48.3% and 36.5% at the 1:40 and 1:160 dilution thresholds, respectively, although the seroprevalence of HHV-8 is probably higher in Gabon. These seroprevalences did not differ by sex, age, ethnicity or province. The detection rate of HHV-8 K1 sequence was 2.6% by PCR. Most of the 31 HHV-8 strains belonged to the B genotype (24), while the remaining clustered within the A5 subgroup (6) and one belonged to the F genotype. Additionally, we reviewed the K1 molecular diversity of published HHV-8 strains in Africa. This study demonstrated a high seroprevalence of HHV-8 in rural adult populations in Gabon and the presence of genetically diverse strains with B, A and also F genotypes. |
| first_indexed | 2024-03-09T03:46:31Z |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:46:31Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-79aa28a49d604bc6bd43ef45a06dfa2e2023-12-03T14:33:29ZengMDPI AGViruses1999-49152021-01-0113217510.3390/v13020175Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in AfricaAntony Idam Mamimandjiami0Augustin Mouinga-Ondémé1Jill-Léa Ramassamy2Délia Doreen Djuicy3Philippe V. Afonso4Antoine Mahé5Jean-Bernard Lekana-Douki6Olivier Cassar7Antoine Gessain8Unité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceUnité des Infections Rétrovirales et Pathologies Associées, Centre International de Recherches Médicales de Franceville, Franceville BP-769, GabonUnité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceUnité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceUnité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceCentre Hospitalier Louis Pasteur, Service de Dermatologie, F-68024 Colmar, FranceEcole Doctorale Régionale d’Afrique Centrale, Infectiologie Tropicale, Franceville BP-876, GabonUnité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceUnité d’Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, CNRS, UMR3569, F-75015 Paris, FranceHuman herpesvirus 8 (HHV-8) is the etiological agent of all forms of Kaposi’s sarcoma (KS). K1 gene studies have identified five major molecular genotypes with geographical clustering. This study described the epidemiology of HHV-8 and its molecular diversity in Gabon among Bantu and Pygmy adult rural populations and KS patients. Plasma antibodies against latency-associated nuclear antigens (LANA) were searched by indirect immunofluorescence. Buffy coat DNA samples were subjected to polymerase chain reaction (PCR) to obtain a K1 gene fragment. We studied 1020 persons; 91% were Bantus and 9% Pygmies. HHV-8 seroprevalence was 48.3% and 36.5% at the 1:40 and 1:160 dilution thresholds, respectively, although the seroprevalence of HHV-8 is probably higher in Gabon. These seroprevalences did not differ by sex, age, ethnicity or province. The detection rate of HHV-8 K1 sequence was 2.6% by PCR. Most of the 31 HHV-8 strains belonged to the B genotype (24), while the remaining clustered within the A5 subgroup (6) and one belonged to the F genotype. Additionally, we reviewed the K1 molecular diversity of published HHV-8 strains in Africa. This study demonstrated a high seroprevalence of HHV-8 in rural adult populations in Gabon and the presence of genetically diverse strains with B, A and also F genotypes.https://www.mdpi.com/1999-4915/13/2/175HHV-8KSHVepidemiologyserologygenetic variabilityrural populations Gabon |
| spellingShingle | Antony Idam Mamimandjiami Augustin Mouinga-Ondémé Jill-Léa Ramassamy Délia Doreen Djuicy Philippe V. Afonso Antoine Mahé Jean-Bernard Lekana-Douki Olivier Cassar Antoine Gessain Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa Viruses HHV-8 KSHV epidemiology serology genetic variability rural populations Gabon |
| title | Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa |
| title_full | Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa |
| title_fullStr | Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa |
| title_full_unstemmed | Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa |
| title_short | Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa |
| title_sort | epidemiology and genetic variability of hhv 8 kshv among rural populations and kaposi s sarcoma patients in gabon central africa review of the geographical distribution of hhv 8 k1 genotypes in africa |
| topic | HHV-8 KSHV epidemiology serology genetic variability rural populations Gabon |
| url | https://www.mdpi.com/1999-4915/13/2/175 |
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