Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea
Brassica juncea is an allopolyploid originating from the interspecific hybridization between Brassica rapa and Brassica nigra, which is of multiple usage as a vegetable, oilseed and condiment worldwide. Both vernalization and non-vernalization under long-day photoperiod can promote floral transition...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2019-05-01
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| Series: | Horticultural Plant Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014119300974 |
| _version_ | 1797204054085992448 |
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| author | Ning Zhao Chang Liu Yiqing Meng Zhongyuan Hu Mingfang Zhang Jinghua Yang |
| author_facet | Ning Zhao Chang Liu Yiqing Meng Zhongyuan Hu Mingfang Zhang Jinghua Yang |
| author_sort | Ning Zhao |
| collection | DOAJ |
| description | Brassica juncea is an allopolyploid originating from the interspecific hybridization between Brassica rapa and Brassica nigra, which is of multiple usage as a vegetable, oilseed and condiment worldwide. Both vernalization and non-vernalization under long-day photoperiod can promote floral transition in B. juncea suggesting merged flowering pathways of its ancestors and better environmental adaptability. We identified genome-wide flowering regulatory genes in B. juncea, which include 84 and 79 genes from A and B sub-genomes, respectively. Ka/Ks analysis revealed a purification effect on both photoperiod and vernalization flowering regulation pathways during evolution. Expression profile of those genes during long-day and vernalization treatments suggested BjuACO4, BjuAFT1, BjuBFT4, BjuASOC1 and BjuASOC4 may be the major functional copies of B. juncea flowering regulation. Further functional studies about BjuCOs showed three days delayed flowering time in BjuACO4 or BjuBCO3 silenced plants. Increased transcription of all BjuFLCs in BjuACO4 or BjuBCO3 silenced plants suggested interactions between photoperiod and vernalization pathways governing flowering time. Our findings provided flowering regulating networks in allopolyploid B. juncea. Keywords: Brassica juncea, Allopolyploidy, Flower, Vernalization, Photoperiod |
| first_indexed | 2024-04-24T08:29:07Z |
| format | Article |
| id | doaj.art-79ab4db9effd412b9c65170c9754a2a2 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:29:07Z |
| publishDate | 2019-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-79ab4db9effd412b9c65170c9754a2a22024-04-16T20:42:19ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412019-05-0153109119Identification of Flowering Regulatory Genes in Allopolyploid Brassica junceaNing Zhao0Chang Liu1Yiqing Meng2Zhongyuan Hu3Mingfang Zhang4Jinghua Yang5Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, ChinaLaboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China; Key laboratory of Horticultural Plant Growth, Development & Quality Improvement, Ministry of Agriculture, Hangzhou 310058, ChinaLaboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China; Key laboratory of Horticultural Plant Growth, Development & Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China; Corresponding author. Tel.: +86 571 88982123Brassica juncea is an allopolyploid originating from the interspecific hybridization between Brassica rapa and Brassica nigra, which is of multiple usage as a vegetable, oilseed and condiment worldwide. Both vernalization and non-vernalization under long-day photoperiod can promote floral transition in B. juncea suggesting merged flowering pathways of its ancestors and better environmental adaptability. We identified genome-wide flowering regulatory genes in B. juncea, which include 84 and 79 genes from A and B sub-genomes, respectively. Ka/Ks analysis revealed a purification effect on both photoperiod and vernalization flowering regulation pathways during evolution. Expression profile of those genes during long-day and vernalization treatments suggested BjuACO4, BjuAFT1, BjuBFT4, BjuASOC1 and BjuASOC4 may be the major functional copies of B. juncea flowering regulation. Further functional studies about BjuCOs showed three days delayed flowering time in BjuACO4 or BjuBCO3 silenced plants. Increased transcription of all BjuFLCs in BjuACO4 or BjuBCO3 silenced plants suggested interactions between photoperiod and vernalization pathways governing flowering time. Our findings provided flowering regulating networks in allopolyploid B. juncea. Keywords: Brassica juncea, Allopolyploidy, Flower, Vernalization, Photoperiodhttp://www.sciencedirect.com/science/article/pii/S2468014119300974 |
| spellingShingle | Ning Zhao Chang Liu Yiqing Meng Zhongyuan Hu Mingfang Zhang Jinghua Yang Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea Horticultural Plant Journal |
| title | Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea |
| title_full | Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea |
| title_fullStr | Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea |
| title_full_unstemmed | Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea |
| title_short | Identification of Flowering Regulatory Genes in Allopolyploid Brassica juncea |
| title_sort | identification of flowering regulatory genes in allopolyploid brassica juncea |
| url | http://www.sciencedirect.com/science/article/pii/S2468014119300974 |
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