MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015
Peste des petits ruminants (PPR) is one of highly contagious viral diseases of small ruminants with high economic losses due to the high morbidity and mortality. In Egypt, PPR in the last 10 years re-emerged again with high mortality in sheep flocks. There is no much data regarding the circulating...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2018-12-01
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| Series: | Slovenian Veterinary Research |
| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/237 |
| _version_ | 1797224948568162304 |
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| author | Wagdy R. ElAshmawy Abdelhamid I Bazid Mohamed Aboelkhair Mostafa A Sakr Aysam M Fayed Mohamed Fawzy |
| author_facet | Wagdy R. ElAshmawy Abdelhamid I Bazid Mohamed Aboelkhair Mostafa A Sakr Aysam M Fayed Mohamed Fawzy |
| author_sort | Wagdy R. ElAshmawy |
| collection | DOAJ |
| description |
Peste des petits ruminants (PPR) is one of highly contagious viral diseases of small ruminants with high economic losses due to the high morbidity and mortality. In Egypt, PPR in the last 10 years re-emerged again with high mortality in sheep flocks. There is no much data regarding the circulating Peste des petits ruminants’ virus and the epidemiological distribution in small ruminants. The study was carried out on a sheep flock of 50 rams aged of 9-12 months with clinical signs suggestive to PPR infection (fever, erosions of the buccal mucosa, pneumonia, diarrhea high morbidity and mortality). Buffy coat and lymph nodes from diseased and dead animals were sent for diagnosis and molecular diagnosis was confirmed using RT-PCR with specific primers targeting three genes; nucleo-capsid (N), fusion (F), and hemagglutinin (H). Sequencing and phylogenetic analysis was carried out on the PCR products and revealed that, the circulating virus is belonged to lineage IV along with Ethiopian strain.
Key words: peste des petits ruminants; molecular; epidemiological; diagnosis; Egypt
MOLEKULARNA EPIDEMIOLOŠKA ŠTUDIJA KUGE DROBNICE V EGIPTU LETA 2015
Povzetek: Kuga drobnice (PPR; iz angl. peste des petits) je ena izmed zelo nalezljivih virusnih bolezni malih prežvekovalcev, ki zaradi visoke obolevnosti in umrljivosti povzroča visoke gospodarske izgube. V Egiptu se je v zadnjih desetih letih PPR znova pojavila z visoko smrtnostjo pri ovcah. O kugi drobnice in epidemiologiji te bolezni pri malih prežvekovalcih v literaturi ne najdemo veliko podatkov. Opisana raziskava je bila opravljena v čredi 50 ovnov, starih od 9 do 12 mesecev, s kliničnimi znaki, ki so kazali na prisotnost bolezni PPR (zvišana telesna temperatura, erozije ustne sluznice, pljučnica, driska, visoka obolevnost in smrtnost). Za natančno diagnosticiranje bolezni je bil uporabljen del krvi, pridobljen s centrifugiranjem, ki vsebuje veliko levkocitov in trombocitov (angl. Buffy coat) in bezgavke obolelih in mrtvih ovnov. Z uporabo specifičnih oligonukleotidov za določanje prisotnosti nukleotidne (N), fuzijske (F) in hemaglutininske (H). beljakovine v reakciji RT PCR smo na molekularnem nivoju potrdili prisotnost okužbe s povzročiteljem PPR. Analiza zaporedja genov in filogenetske analize so bile izvedene na produktih analize PCR, pri tem pa smo ugotovili, da virus pripada liniji IV skupaj z etiopijskim sevom.
Ključne besede: kuga drobnice; molekularna diagnostika; epidemiologija; Egipt
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| first_indexed | 2024-04-24T14:01:14Z |
| format | Article |
| id | doaj.art-79acc952982f4cfdac6cbf526c1be700 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:01:14Z |
| publishDate | 2018-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-79acc952982f4cfdac6cbf526c1be7002024-04-03T12:39:51ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612018-12-0155410.26873/SVR-237-2018MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015Wagdy R. ElAshmawy0Abdelhamid I Bazid1Mohamed Aboelkhair2Mostafa A Sakr3Aysam M Fayed4Mohamed Fawzy5Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Corresponding Author, 00201144911754, ubiowagdy@staff.cu.edu.eg.Department of Virology, Faculty of Veterinary Medicine, University of Sadat City, Sadat city, MinoufiyaDepartment of Virology, Faculty of Veterinary Medicine, University of Sadat City, Sadat city, MinoufiyaMolecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat CityMolecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat CityDepartment of Virology, Faculty of Veterinary Medicine- Suez Canal University, Ismailia, Egypt Peste des petits ruminants (PPR) is one of highly contagious viral diseases of small ruminants with high economic losses due to the high morbidity and mortality. In Egypt, PPR in the last 10 years re-emerged again with high mortality in sheep flocks. There is no much data regarding the circulating Peste des petits ruminants’ virus and the epidemiological distribution in small ruminants. The study was carried out on a sheep flock of 50 rams aged of 9-12 months with clinical signs suggestive to PPR infection (fever, erosions of the buccal mucosa, pneumonia, diarrhea high morbidity and mortality). Buffy coat and lymph nodes from diseased and dead animals were sent for diagnosis and molecular diagnosis was confirmed using RT-PCR with specific primers targeting three genes; nucleo-capsid (N), fusion (F), and hemagglutinin (H). Sequencing and phylogenetic analysis was carried out on the PCR products and revealed that, the circulating virus is belonged to lineage IV along with Ethiopian strain. Key words: peste des petits ruminants; molecular; epidemiological; diagnosis; Egypt MOLEKULARNA EPIDEMIOLOŠKA ŠTUDIJA KUGE DROBNICE V EGIPTU LETA 2015 Povzetek: Kuga drobnice (PPR; iz angl. peste des petits) je ena izmed zelo nalezljivih virusnih bolezni malih prežvekovalcev, ki zaradi visoke obolevnosti in umrljivosti povzroča visoke gospodarske izgube. V Egiptu se je v zadnjih desetih letih PPR znova pojavila z visoko smrtnostjo pri ovcah. O kugi drobnice in epidemiologiji te bolezni pri malih prežvekovalcih v literaturi ne najdemo veliko podatkov. Opisana raziskava je bila opravljena v čredi 50 ovnov, starih od 9 do 12 mesecev, s kliničnimi znaki, ki so kazali na prisotnost bolezni PPR (zvišana telesna temperatura, erozije ustne sluznice, pljučnica, driska, visoka obolevnost in smrtnost). Za natančno diagnosticiranje bolezni je bil uporabljen del krvi, pridobljen s centrifugiranjem, ki vsebuje veliko levkocitov in trombocitov (angl. Buffy coat) in bezgavke obolelih in mrtvih ovnov. Z uporabo specifičnih oligonukleotidov za določanje prisotnosti nukleotidne (N), fuzijske (F) in hemaglutininske (H). beljakovine v reakciji RT PCR smo na molekularnem nivoju potrdili prisotnost okužbe s povzročiteljem PPR. Analiza zaporedja genov in filogenetske analize so bile izvedene na produktih analize PCR, pri tem pa smo ugotovili, da virus pripada liniji IV skupaj z etiopijskim sevom. Ključne besede: kuga drobnice; molekularna diagnostika; epidemiologija; Egipt https://www.slovetres.si/index.php/SVR/article/view/237 |
| spellingShingle | Wagdy R. ElAshmawy Abdelhamid I Bazid Mohamed Aboelkhair Mostafa A Sakr Aysam M Fayed Mohamed Fawzy MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 Slovenian Veterinary Research |
| title | MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 |
| title_full | MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 |
| title_fullStr | MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 |
| title_full_unstemmed | MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 |
| title_short | MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015 |
| title_sort | molecular epidemiological study on peste des petits ruminants in egypt 2015 |
| url | https://www.slovetres.si/index.php/SVR/article/view/237 |
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