Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration
Abstract Background Andrographis producta (C. B. Clarke) Gamble is a valuable medicinal plant that yields several therapeutic compounds. In addition, this species is endemic to the Western Ghats regions of South India. Natural populations of Andrographis producta have dwindled due to the overexploit...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-022-00438-w |
| _version_ | 1827280436497219584 |
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| author | Sathish Shekhappa Kadapatti Hosakatte Niranjana Murthy |
| author_facet | Sathish Shekhappa Kadapatti Hosakatte Niranjana Murthy |
| author_sort | Sathish Shekhappa Kadapatti |
| collection | DOAJ |
| description | Abstract Background Andrographis producta (C. B. Clarke) Gamble is a valuable medicinal plant that yields several therapeutic compounds. In addition, this species is endemic to the Western Ghats regions of South India. Natural populations of Andrographis producta have dwindled due to the overexploitation of this species. The objective of the present study was to develop a reliable In vitro propagation protocol for this plant species. Results In vitro plant regeneration protocol has been developed in Andrographis producta using nodal and shoot tip explants. The highest axillary shoots (14.60) were regenerated from nodal explants on MS medium amended with 10 μM 2-iP. Similarly, on MS media amended with 5 μM BAP, 17.50 shoots were regenerated from shoot tip explants. Optimal of 27.66 adventitious shoots were regenerated from the cut end of shoot tip explants on MS medium amended with 10 μM 2-iP. Medium amended with 10 μM 2-iP was optimum for regeneration of multiple axillary shoots from nodal explants and for the adventitious shoots regeneration from shoot tip explants. Shoot tips were ideal explants for the micropropagation of A. producta. Qarter sthrength MS media supplemented with 10 μM IBA has resulted in maximum rooting of the shoots. Conclusions A reliable In vitro micropropagation method was developed in Andrographis producta through direct organogenesis, and this method is helpful for the multiplication, conservation, and utilization of this plant. |
| first_indexed | 2024-04-11T23:03:51Z |
| format | Article |
| id | doaj.art-79b0bada50f143a0b6c3bd6e6a72ecb2 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:41:10Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-79b0bada50f143a0b6c3bd6e6a72ecb22024-04-16T16:01:28ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-11-012011810.1186/s43141-022-00438-wMicropropagation of Andrographis producta through axillary and adventitious shoot regenerationSathish Shekhappa Kadapatti0Hosakatte Niranjana Murthy1Department of Botany, Karnatak UniversityDepartment of Botany, Karnatak UniversityAbstract Background Andrographis producta (C. B. Clarke) Gamble is a valuable medicinal plant that yields several therapeutic compounds. In addition, this species is endemic to the Western Ghats regions of South India. Natural populations of Andrographis producta have dwindled due to the overexploitation of this species. The objective of the present study was to develop a reliable In vitro propagation protocol for this plant species. Results In vitro plant regeneration protocol has been developed in Andrographis producta using nodal and shoot tip explants. The highest axillary shoots (14.60) were regenerated from nodal explants on MS medium amended with 10 μM 2-iP. Similarly, on MS media amended with 5 μM BAP, 17.50 shoots were regenerated from shoot tip explants. Optimal of 27.66 adventitious shoots were regenerated from the cut end of shoot tip explants on MS medium amended with 10 μM 2-iP. Medium amended with 10 μM 2-iP was optimum for regeneration of multiple axillary shoots from nodal explants and for the adventitious shoots regeneration from shoot tip explants. Shoot tips were ideal explants for the micropropagation of A. producta. Qarter sthrength MS media supplemented with 10 μM IBA has resulted in maximum rooting of the shoots. Conclusions A reliable In vitro micropropagation method was developed in Andrographis producta through direct organogenesis, and this method is helpful for the multiplication, conservation, and utilization of this plant.https://doi.org/10.1186/s43141-022-00438-wAdventitious shootsAndrographis productaAxillary shootsIn vitro regeneration |
| spellingShingle | Sathish Shekhappa Kadapatti Hosakatte Niranjana Murthy Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration Journal of Genetic Engineering and Biotechnology Adventitious shoots Andrographis producta Axillary shoots In vitro regeneration |
| title | Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration |
| title_full | Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration |
| title_fullStr | Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration |
| title_full_unstemmed | Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration |
| title_short | Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration |
| title_sort | micropropagation of andrographis producta through axillary and adventitious shoot regeneration |
| topic | Adventitious shoots Andrographis producta Axillary shoots In vitro regeneration |
| url | https://doi.org/10.1186/s43141-022-00438-w |
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