T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population
Complication of retinopathy in type 2 DM is caused of lower level of NO. Nitric oxide level is synthesized from L-arginin in reaction that catalyze Nitric oxide synthase (NOS) 3. The T-786C mutation in NOS 3 gene decreases the expression of nitric oxide synthase (NOS) 3 so decreases NO synthesis. To...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2011-12-01
|
| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/16372 |
| _version_ | 1818484365945995264 |
|---|---|
| author | Putri Widelia Welkriana S. Sunarti Pramudji Hastuti |
| author_facet | Putri Widelia Welkriana S. Sunarti Pramudji Hastuti |
| author_sort | Putri Widelia Welkriana |
| collection | DOAJ |
| description | Complication of retinopathy in type 2 DM is caused of lower level of NO. Nitric oxide level is synthesized
from L-arginin in reaction that catalyze Nitric oxide synthase (NOS) 3. The T-786C mutation in NOS 3 gene
decreases the expression of nitric oxide synthase (NOS) 3 so decreases NO synthesis. To investigate the
association between T-786C polymorphism in NOS 3 gene with NO level of diabetic retinopathy patients. This
study was a case control study, consist of 40 patient of type 2 diabetic with DR (case group) and 40 patient of
type 2 diabetic without DR (control group) of Javanese ethnic. The genotyping of T-786C polymorphism was
performed by PCR-RLFP. Level of NO was measured by spectrophotometry. Chi square test and odd ratio
were used to analyze the association of the T-786C polymorphism in NOS 3 gene with DR. Differences of
NO level between TT and TC genotypes were analyzed using independent t test. The distribution of T-786C
polymorphism in NOS 3 gene of DR subjects showed that frequency of TT genotype was 22.5% and TC genotype
was 77.5%. Non DR subjects showed the frequency of TT genotype was 50% and TC genotype was 50%, (p=
0.011). Frequency of T allele in DR group was 61.25% and C allele was 38.75%, and frequency of T allele in non
DR group was 75% and C allele was 25%, (p= 0.62). Odd ratio of TC genotype was 3.444(CI; 95% : 0.964-3.735)
and C allele was 1.898 (CI; 95% : 1.310-9.058). The NO level of TC genotype was 1.43+0.126 and TT genotype
was 11.27+5.87 (p=0.000). Level of NO between RD and non RD showed not different significantly (p=0.160)
for retinopathy. The T-786C polymorphism of NOS 3 gene is risk factor for retinopathy in type 2 Diabetes
Mellitus. Individual with TC genotype of NOS 3 gene has lower level of NO than TT genotype. |
| first_indexed | 2024-12-10T15:54:22Z |
| format | Article |
| id | doaj.art-79b138fc02db473c9581c2fa68414eb5 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-10T15:54:22Z |
| publishDate | 2011-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-79b138fc02db473c9581c2fa68414eb52022-12-22T01:42:41ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412011-12-0116212613110.22146/ijbiotech.1637211390T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese PopulationPutri Widelia Welkriana0S. Sunarti1Pramudji Hastuti2Postgraduated Program of Basic and Biomedical Science, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, IndonesiaComplication of retinopathy in type 2 DM is caused of lower level of NO. Nitric oxide level is synthesized from L-arginin in reaction that catalyze Nitric oxide synthase (NOS) 3. The T-786C mutation in NOS 3 gene decreases the expression of nitric oxide synthase (NOS) 3 so decreases NO synthesis. To investigate the association between T-786C polymorphism in NOS 3 gene with NO level of diabetic retinopathy patients. This study was a case control study, consist of 40 patient of type 2 diabetic with DR (case group) and 40 patient of type 2 diabetic without DR (control group) of Javanese ethnic. The genotyping of T-786C polymorphism was performed by PCR-RLFP. Level of NO was measured by spectrophotometry. Chi square test and odd ratio were used to analyze the association of the T-786C polymorphism in NOS 3 gene with DR. Differences of NO level between TT and TC genotypes were analyzed using independent t test. The distribution of T-786C polymorphism in NOS 3 gene of DR subjects showed that frequency of TT genotype was 22.5% and TC genotype was 77.5%. Non DR subjects showed the frequency of TT genotype was 50% and TC genotype was 50%, (p= 0.011). Frequency of T allele in DR group was 61.25% and C allele was 38.75%, and frequency of T allele in non DR group was 75% and C allele was 25%, (p= 0.62). Odd ratio of TC genotype was 3.444(CI; 95% : 0.964-3.735) and C allele was 1.898 (CI; 95% : 1.310-9.058). The NO level of TC genotype was 1.43+0.126 and TT genotype was 11.27+5.87 (p=0.000). Level of NO between RD and non RD showed not different significantly (p=0.160) for retinopathy. The T-786C polymorphism of NOS 3 gene is risk factor for retinopathy in type 2 Diabetes Mellitus. Individual with TC genotype of NOS 3 gene has lower level of NO than TT genotype.https://jurnal.ugm.ac.id/ijbiotech/article/view/16372Diabetic RetinopathyPolymorphismNitric OxideNitric Oxide Synthase |
| spellingShingle | Putri Widelia Welkriana S. Sunarti Pramudji Hastuti T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population Indonesian Journal of Biotechnology Diabetic Retinopathy Polymorphism Nitric Oxide Nitric Oxide Synthase |
| title | T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population |
| title_full | T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population |
| title_fullStr | T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population |
| title_full_unstemmed | T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population |
| title_short | T-786c Polymorphism in nitric oxide synthase 3 gene and Nitrit Oxide Level of Diabetic Retinopathy in Javanese Population |
| title_sort | t 786c polymorphism in nitric oxide synthase 3 gene and nitrit oxide level of diabetic retinopathy in javanese population |
| topic | Diabetic Retinopathy Polymorphism Nitric Oxide Nitric Oxide Synthase |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/16372 |
| work_keys_str_mv | AT putriwideliawelkriana t786cpolymorphisminnitricoxidesynthase3geneandnitritoxidelevelofdiabeticretinopathyinjavanesepopulation AT ssunarti t786cpolymorphisminnitricoxidesynthase3geneandnitritoxidelevelofdiabeticretinopathyinjavanesepopulation AT pramudjihastuti t786cpolymorphisminnitricoxidesynthase3geneandnitritoxidelevelofdiabeticretinopathyinjavanesepopulation |