Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo
The extensive use of carbamate pesticides has led to a range of environmental and health problems, such as surface and groundwater contamination, and endocrine disorders in organisms. In this study, we focused on examining the effects of toxic exposure to the carbamate pesticide methomyl on the hatc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323011880 |
| _version_ | 1797435858644631552 |
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| author | Shun Long Meng Ming Xiao Li Yan Lu Xi Chen Wei Ping Wang Chao Song Li Min Fan Li Ping Qiu Dan Dan Li Hui Min Xu Pao Xu |
| author_facet | Shun Long Meng Ming Xiao Li Yan Lu Xi Chen Wei Ping Wang Chao Song Li Min Fan Li Ping Qiu Dan Dan Li Hui Min Xu Pao Xu |
| author_sort | Shun Long Meng |
| collection | DOAJ |
| description | The extensive use of carbamate pesticides has led to a range of environmental and health problems, such as surface and groundwater contamination, and endocrine disorders in organisms. In this study, we focused on examining the effects of toxic exposure to the carbamate pesticide methomyl on the hatching, morphology, immunity and developmental gene expression levels in zebrafish embryos. Four concentrations of methomyl (0, 2, 20, and 200 μg/L) were administered to zebrafish embryos for a period of 96 h. The study found that exposure to methomyl accelerated the hatching process of zebrafish embryos, with the strongest effect recorded at the concentration of 2 μg/L. Methomyl exposure also trigged significantly reductions in heart rate and caused abnormalities in larvae morphology, and it also stimulated the synthesis and release of several inflammatory factors such as IL-1β, IL-6, TNF-α and INF-α, lowered the IgM contents, ultimately enhancing inflammatory response and interfering with immune function. All of these showed the significant effects on exposure time, concentration and their interaction (Time × Concentration). Furthermore, the body length of zebrafish exposed to methomyl for 96 h was significantly shorter, particularly at higher concentrations (200 μg/L). Methomyl also affected the expression levels of genes associated with development (down-regulated igf1, bmp2b, vasa, dazl and piwi genes), demonstrating strong developmental toxicity and disruption of the endocrine system, with the most observed at the concentration of 200 μg/L and 96 h exposure to methomyl. The results of this study provide valuable reference information on the potential damage of methomyl concentrations in the environment on fish embryo development, while also supplementing present research on the immunotoxicity of methomyl. |
| first_indexed | 2024-03-09T10:53:26Z |
| format | Article |
| id | doaj.art-79b2dc998cd145cfa2f5809fc2d09b2b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-09T10:53:26Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-79b2dc998cd145cfa2f5809fc2d09b2b2023-12-01T05:00:25ZengElsevierEcotoxicology and Environmental Safety0147-65132023-12-01268115684Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryoShun Long Meng0Ming Xiao Li1Yan Lu2Xi Chen3Wei Ping Wang4Chao Song5Li Min Fan6Li Ping Qiu7Dan Dan Li8Hui Min Xu9Pao Xu10Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, China; Corresponding authors at: Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China.Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaWuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaJiangxi Provincial Aquatic Biology Protection and Rescue Center, Nangchang 330029, ChinaWuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaWuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, ChinaWuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Wuxi 214081, China; Corresponding authors at: Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China.The extensive use of carbamate pesticides has led to a range of environmental and health problems, such as surface and groundwater contamination, and endocrine disorders in organisms. In this study, we focused on examining the effects of toxic exposure to the carbamate pesticide methomyl on the hatching, morphology, immunity and developmental gene expression levels in zebrafish embryos. Four concentrations of methomyl (0, 2, 20, and 200 μg/L) were administered to zebrafish embryos for a period of 96 h. The study found that exposure to methomyl accelerated the hatching process of zebrafish embryos, with the strongest effect recorded at the concentration of 2 μg/L. Methomyl exposure also trigged significantly reductions in heart rate and caused abnormalities in larvae morphology, and it also stimulated the synthesis and release of several inflammatory factors such as IL-1β, IL-6, TNF-α and INF-α, lowered the IgM contents, ultimately enhancing inflammatory response and interfering with immune function. All of these showed the significant effects on exposure time, concentration and their interaction (Time × Concentration). Furthermore, the body length of zebrafish exposed to methomyl for 96 h was significantly shorter, particularly at higher concentrations (200 μg/L). Methomyl also affected the expression levels of genes associated with development (down-regulated igf1, bmp2b, vasa, dazl and piwi genes), demonstrating strong developmental toxicity and disruption of the endocrine system, with the most observed at the concentration of 200 μg/L and 96 h exposure to methomyl. The results of this study provide valuable reference information on the potential damage of methomyl concentrations in the environment on fish embryo development, while also supplementing present research on the immunotoxicity of methomyl.http://www.sciencedirect.com/science/article/pii/S0147651323011880MethomylEcotoxicologyZebrafish embryoEmbryo morphologyDevelop associated genes |
| spellingShingle | Shun Long Meng Ming Xiao Li Yan Lu Xi Chen Wei Ping Wang Chao Song Li Min Fan Li Ping Qiu Dan Dan Li Hui Min Xu Pao Xu Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo Ecotoxicology and Environmental Safety Methomyl Ecotoxicology Zebrafish embryo Embryo morphology Develop associated genes |
| title | Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo |
| title_full | Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo |
| title_fullStr | Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo |
| title_full_unstemmed | Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo |
| title_short | Effect of environmental level of methomyl on hatching, morphology, immunity and development related genes expression in zebrafish (Danio rerio) embryo |
| title_sort | effect of environmental level of methomyl on hatching morphology immunity and development related genes expression in zebrafish danio rerio embryo |
| topic | Methomyl Ecotoxicology Zebrafish embryo Embryo morphology Develop associated genes |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323011880 |
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