| Summary: | Shared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying plasmids collected from shared bikes is still lacking. Here, we used the HiSeq platform to sequence and analyze 24 <i>Escherichia coli</i> isolated from shared bikes around Metro Stations in Beijing. The isolates from shared bikes showed 14 STs and various genotypes. Two <i>bla</i><sub>NDM-5</sub> and <i>bla</i><sub>CTX-M-199</sub>-producing ST167 <i>E. coli</i> have 16 resistance genes, four plasmid types and show >95% of similarities in core genomes compared with the ST167 <i>E. coli</i> strains from different origins. The <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub>-carrying plasmids sequencing by Nanopore were compared to plasmids with <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub> originated from humans and animals. These two ST167 <i>E. coli</i> show high similarities in core genomes and the plasmid profiles with strains from hospital inpatients and farm animals. Our study indicated that ST167 <i>E. coli</i> is retained in diverse environments and carried with various plasmids. The analysis of strains such as ST167 can provide useful information for preventing or controlling the spread of AR bacteria between animals, humans and environments.
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