Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes
Shared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying pl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/8/1030 |
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| author | Qiyan Chen Zhiyu Zou Chang Cai Hui Li Yang Wang Lei Lei Bing Shao |
| author_facet | Qiyan Chen Zhiyu Zou Chang Cai Hui Li Yang Wang Lei Lei Bing Shao |
| author_sort | Qiyan Chen |
| collection | DOAJ |
| description | Shared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying plasmids collected from shared bikes is still lacking. Here, we used the HiSeq platform to sequence and analyze 24 <i>Escherichia coli</i> isolated from shared bikes around Metro Stations in Beijing. The isolates from shared bikes showed 14 STs and various genotypes. Two <i>bla</i><sub>NDM-5</sub> and <i>bla</i><sub>CTX-M-199</sub>-producing ST167 <i>E. coli</i> have 16 resistance genes, four plasmid types and show >95% of similarities in core genomes compared with the ST167 <i>E. coli</i> strains from different origins. The <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub>-carrying plasmids sequencing by Nanopore were compared to plasmids with <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub> originated from humans and animals. These two ST167 <i>E. coli</i> show high similarities in core genomes and the plasmid profiles with strains from hospital inpatients and farm animals. Our study indicated that ST167 <i>E. coli</i> is retained in diverse environments and carried with various plasmids. The analysis of strains such as ST167 can provide useful information for preventing or controlling the spread of AR bacteria between animals, humans and environments. |
| first_indexed | 2024-03-09T11:59:37Z |
| format | Article |
| id | doaj.art-79b632bc9bde41ff9961b77ad8633562 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:59:37Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-79b632bc9bde41ff9961b77ad86335622023-11-30T23:04:40ZengMDPI AGAntibiotics2079-63822022-07-01118103010.3390/antibiotics11081030Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared BikesQiyan Chen0Zhiyu Zou1Chang Cai2Hui Li3Yang Wang4Lei Lei5Bing Shao6Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaCollege of Arts, Business, Law and Social Sciences, Murdoch University, Perth, WA 6150, AustraliaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, ChinaBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaShared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying plasmids collected from shared bikes is still lacking. Here, we used the HiSeq platform to sequence and analyze 24 <i>Escherichia coli</i> isolated from shared bikes around Metro Stations in Beijing. The isolates from shared bikes showed 14 STs and various genotypes. Two <i>bla</i><sub>NDM-5</sub> and <i>bla</i><sub>CTX-M-199</sub>-producing ST167 <i>E. coli</i> have 16 resistance genes, four plasmid types and show >95% of similarities in core genomes compared with the ST167 <i>E. coli</i> strains from different origins. The <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub>-carrying plasmids sequencing by Nanopore were compared to plasmids with <i>bla</i><sub>NDM-5</sub>- or <i>bla</i><sub>CTX-M-199</sub> originated from humans and animals. These two ST167 <i>E. coli</i> show high similarities in core genomes and the plasmid profiles with strains from hospital inpatients and farm animals. Our study indicated that ST167 <i>E. coli</i> is retained in diverse environments and carried with various plasmids. The analysis of strains such as ST167 can provide useful information for preventing or controlling the spread of AR bacteria between animals, humans and environments.https://www.mdpi.com/2079-6382/11/8/1030shared bikesNDM-5CTX-M-199ST167whole genome analysis |
| spellingShingle | Qiyan Chen Zhiyu Zou Chang Cai Hui Li Yang Wang Lei Lei Bing Shao Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes Antibiotics shared bikes NDM-5 CTX-M-199 ST167 whole genome analysis |
| title | Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes |
| title_full | Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes |
| title_fullStr | Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes |
| title_full_unstemmed | Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes |
| title_short | Characterization of <i>bla</i><sub>NDM-5</sub>-and <i>bla</i><sub>CTX-M-199</sub>-Producing ST167 <i>Escherichia coli</i> Isolated from Shared Bikes |
| title_sort | characterization of i bla i sub ndm 5 sub and i bla i sub ctx m 199 sub producing st167 i escherichia coli i isolated from shared bikes |
| topic | shared bikes NDM-5 CTX-M-199 ST167 whole genome analysis |
| url | https://www.mdpi.com/2079-6382/11/8/1030 |
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